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Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials

Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Rec...

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Autores principales: Szczepankowska, Agnieszka K., Szatraj, Katarzyna, Sałański, Przemysław, Rózga, Agnieszka, Górecki, Roman K., Bardowski, Jacek K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340954/
https://www.ncbi.nlm.nih.gov/pubmed/28321412
http://dx.doi.org/10.1155/2017/6747482
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author Szczepankowska, Agnieszka K.
Szatraj, Katarzyna
Sałański, Przemysław
Rózga, Agnieszka
Górecki, Roman K.
Bardowski, Jacek K.
author_facet Szczepankowska, Agnieszka K.
Szatraj, Katarzyna
Sałański, Przemysław
Rózga, Agnieszka
Górecki, Roman K.
Bardowski, Jacek K.
author_sort Szczepankowska, Agnieszka K.
collection PubMed
description Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface.
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spelling pubmed-53409542017-03-20 Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials Szczepankowska, Agnieszka K. Szatraj, Katarzyna Sałański, Przemysław Rózga, Agnieszka Górecki, Roman K. Bardowski, Jacek K. Biomed Res Int Research Article Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis. Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface. Hindawi Publishing Corporation 2017 2017-02-22 /pmc/articles/PMC5340954/ /pubmed/28321412 http://dx.doi.org/10.1155/2017/6747482 Text en Copyright © 2017 Agnieszka K. Szczepankowska et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Szczepankowska, Agnieszka K.
Szatraj, Katarzyna
Sałański, Przemysław
Rózga, Agnieszka
Górecki, Roman K.
Bardowski, Jacek K.
Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title_full Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title_fullStr Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title_full_unstemmed Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title_short Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials
title_sort recombinant lactococcus lactis expressing haemagglutinin from a polish avian h5n1 isolate and its immunological effect in preliminary animal trials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340954/
https://www.ncbi.nlm.nih.gov/pubmed/28321412
http://dx.doi.org/10.1155/2017/6747482
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