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Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface

Organismal function is, to a great extent, determined by interactions among their fundamental building blocks, the cells. In this work, we studied the cell-cell interactome of fetal placental trophoblast cells and maternal endometrial stromal cells, using single-cell transcriptomics. The placental i...

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Autores principales: Pavličev, Mihaela, Wagner, Günter P., Chavan, Arun Rajendra, Owens, Kathryn, Maziarz, Jamie, Dunn-Fletcher, Caitlin, Kallapur, Suhas G., Muglia, Louis, Jones, Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340963/
https://www.ncbi.nlm.nih.gov/pubmed/28174237
http://dx.doi.org/10.1101/gr.207597.116
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author Pavličev, Mihaela
Wagner, Günter P.
Chavan, Arun Rajendra
Owens, Kathryn
Maziarz, Jamie
Dunn-Fletcher, Caitlin
Kallapur, Suhas G.
Muglia, Louis
Jones, Helen
author_facet Pavličev, Mihaela
Wagner, Günter P.
Chavan, Arun Rajendra
Owens, Kathryn
Maziarz, Jamie
Dunn-Fletcher, Caitlin
Kallapur, Suhas G.
Muglia, Louis
Jones, Helen
author_sort Pavličev, Mihaela
collection PubMed
description Organismal function is, to a great extent, determined by interactions among their fundamental building blocks, the cells. In this work, we studied the cell-cell interactome of fetal placental trophoblast cells and maternal endometrial stromal cells, using single-cell transcriptomics. The placental interface mediates the interaction between two semiallogenic individuals, the mother and the fetus, and is thus the epitome of cell interactions. To study these, we inferred the cell-cell interactome by assessing the gene expression of receptor-ligand pairs across cell types. We find a highly cell-type-specific expression of G-protein-coupled receptors, implying that ligand-receptor profiles could be a reliable tool for cell type identification. Furthermore, we find that uterine decidual cells represent a cell-cell interaction hub with a large number of potential incoming and outgoing signals. Decidual cells differentiate from their precursors, the endometrial stromal fibroblasts, during uterine preparation for pregnancy. We show that decidualization (even in vitro) enhances the ability to communicate with the fetus, as most of the receptors and ligands up-regulated during decidualization have their counterpart expressed in trophoblast cells. Among the signals transmitted, growth factors and immune signals dominate, and suggest a delicate balance of enhancing and suppressive signals. Finally, this study provides a rich resource of gene expression profiles of term intravillous and extravillous trophoblasts, including the transcriptome of the multinucleated syncytiotrophoblast.
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spelling pubmed-53409632017-09-01 Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface Pavličev, Mihaela Wagner, Günter P. Chavan, Arun Rajendra Owens, Kathryn Maziarz, Jamie Dunn-Fletcher, Caitlin Kallapur, Suhas G. Muglia, Louis Jones, Helen Genome Res Research Organismal function is, to a great extent, determined by interactions among their fundamental building blocks, the cells. In this work, we studied the cell-cell interactome of fetal placental trophoblast cells and maternal endometrial stromal cells, using single-cell transcriptomics. The placental interface mediates the interaction between two semiallogenic individuals, the mother and the fetus, and is thus the epitome of cell interactions. To study these, we inferred the cell-cell interactome by assessing the gene expression of receptor-ligand pairs across cell types. We find a highly cell-type-specific expression of G-protein-coupled receptors, implying that ligand-receptor profiles could be a reliable tool for cell type identification. Furthermore, we find that uterine decidual cells represent a cell-cell interaction hub with a large number of potential incoming and outgoing signals. Decidual cells differentiate from their precursors, the endometrial stromal fibroblasts, during uterine preparation for pregnancy. We show that decidualization (even in vitro) enhances the ability to communicate with the fetus, as most of the receptors and ligands up-regulated during decidualization have their counterpart expressed in trophoblast cells. Among the signals transmitted, growth factors and immune signals dominate, and suggest a delicate balance of enhancing and suppressive signals. Finally, this study provides a rich resource of gene expression profiles of term intravillous and extravillous trophoblasts, including the transcriptome of the multinucleated syncytiotrophoblast. Cold Spring Harbor Laboratory Press 2017-03 /pmc/articles/PMC5340963/ /pubmed/28174237 http://dx.doi.org/10.1101/gr.207597.116 Text en © 2017 Pavličev et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Pavličev, Mihaela
Wagner, Günter P.
Chavan, Arun Rajendra
Owens, Kathryn
Maziarz, Jamie
Dunn-Fletcher, Caitlin
Kallapur, Suhas G.
Muglia, Louis
Jones, Helen
Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title_full Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title_fullStr Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title_full_unstemmed Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title_short Single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
title_sort single-cell transcriptomics of the human placenta: inferring the cell communication network of the maternal-fetal interface
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340963/
https://www.ncbi.nlm.nih.gov/pubmed/28174237
http://dx.doi.org/10.1101/gr.207597.116
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