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Ultrastructural Characterization of Zika Virus Replication Factories

A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques t...

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Autores principales: Cortese, Mirko, Goellner, Sarah, Acosta, Eliana Gisela, Neufeldt, Christopher John, Oleksiuk, Olga, Lampe, Marko, Haselmann, Uta, Funaya, Charlotta, Schieber, Nicole, Ronchi, Paolo, Schorb, Martin, Pruunsild, Priit, Schwab, Yannick, Chatel-Chaix, Laurent, Ruggieri, Alessia, Bartenschlager, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340982/
https://www.ncbi.nlm.nih.gov/pubmed/28249158
http://dx.doi.org/10.1016/j.celrep.2017.02.014
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author Cortese, Mirko
Goellner, Sarah
Acosta, Eliana Gisela
Neufeldt, Christopher John
Oleksiuk, Olga
Lampe, Marko
Haselmann, Uta
Funaya, Charlotta
Schieber, Nicole
Ronchi, Paolo
Schorb, Martin
Pruunsild, Priit
Schwab, Yannick
Chatel-Chaix, Laurent
Ruggieri, Alessia
Bartenschlager, Ralf
author_facet Cortese, Mirko
Goellner, Sarah
Acosta, Eliana Gisela
Neufeldt, Christopher John
Oleksiuk, Olga
Lampe, Marko
Haselmann, Uta
Funaya, Charlotta
Schieber, Nicole
Ronchi, Paolo
Schorb, Martin
Pruunsild, Priit
Schwab, Yannick
Chatel-Chaix, Laurent
Ruggieri, Alessia
Bartenschlager, Ralf
author_sort Cortese, Mirko
collection PubMed
description A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.
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spelling pubmed-53409822017-03-13 Ultrastructural Characterization of Zika Virus Replication Factories Cortese, Mirko Goellner, Sarah Acosta, Eliana Gisela Neufeldt, Christopher John Oleksiuk, Olga Lampe, Marko Haselmann, Uta Funaya, Charlotta Schieber, Nicole Ronchi, Paolo Schorb, Martin Pruunsild, Priit Schwab, Yannick Chatel-Chaix, Laurent Ruggieri, Alessia Bartenschlager, Ralf Cell Rep Article A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton. The Author(s). 2017-02-28 2017-02-28 /pmc/articles/PMC5340982/ /pubmed/28249158 http://dx.doi.org/10.1016/j.celrep.2017.02.014 Text en © 2017 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Cortese, Mirko
Goellner, Sarah
Acosta, Eliana Gisela
Neufeldt, Christopher John
Oleksiuk, Olga
Lampe, Marko
Haselmann, Uta
Funaya, Charlotta
Schieber, Nicole
Ronchi, Paolo
Schorb, Martin
Pruunsild, Priit
Schwab, Yannick
Chatel-Chaix, Laurent
Ruggieri, Alessia
Bartenschlager, Ralf
Ultrastructural Characterization of Zika Virus Replication Factories
title Ultrastructural Characterization of Zika Virus Replication Factories
title_full Ultrastructural Characterization of Zika Virus Replication Factories
title_fullStr Ultrastructural Characterization of Zika Virus Replication Factories
title_full_unstemmed Ultrastructural Characterization of Zika Virus Replication Factories
title_short Ultrastructural Characterization of Zika Virus Replication Factories
title_sort ultrastructural characterization of zika virus replication factories
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340982/
https://www.ncbi.nlm.nih.gov/pubmed/28249158
http://dx.doi.org/10.1016/j.celrep.2017.02.014
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