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Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation
Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340989/ https://www.ncbi.nlm.nih.gov/pubmed/28321408 http://dx.doi.org/10.1155/2017/3909657 |
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author | Vijayaraghavan, Ponnuswamy Rajendran, P. Prakash Vincent, Samuel Gnana Arun, Arumugaperumal Abdullah Al-Dhabi, Naif Valan Arasu, Mariadhas Young Kwon, Oh Kim, Young Ock |
author_facet | Vijayaraghavan, Ponnuswamy Rajendran, P. Prakash Vincent, Samuel Gnana Arun, Arumugaperumal Abdullah Al-Dhabi, Naif Valan Arasu, Mariadhas Young Kwon, Oh Kim, Young Ock |
author_sort | Vijayaraghavan, Ponnuswamy |
collection | PubMed |
description | Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO(4) were the vital parameters with statistical significance (p < 0.001). Three factors (moisture, sucrose, and MgSO(4)) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed 4143 ± 12.31 U/g material, which was more than fourfold the initial enzyme production (978 ± 36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p < 0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83 ± 3.6%), egg white (100%), and bovine serum albumin (29 ± 4.9%). |
format | Online Article Text |
id | pubmed-5340989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-53409892017-03-20 Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation Vijayaraghavan, Ponnuswamy Rajendran, P. Prakash Vincent, Samuel Gnana Arun, Arumugaperumal Abdullah Al-Dhabi, Naif Valan Arasu, Mariadhas Young Kwon, Oh Kim, Young Ock Biomed Res Int Research Article Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO(4) were the vital parameters with statistical significance (p < 0.001). Three factors (moisture, sucrose, and MgSO(4)) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed 4143 ± 12.31 U/g material, which was more than fourfold the initial enzyme production (978 ± 36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p < 0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83 ± 3.6%), egg white (100%), and bovine serum albumin (29 ± 4.9%). Hindawi Publishing Corporation 2017 2017-01-17 /pmc/articles/PMC5340989/ /pubmed/28321408 http://dx.doi.org/10.1155/2017/3909657 Text en Copyright © 2017 Ponnuswamy Vijayaraghavan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Vijayaraghavan, Ponnuswamy Rajendran, P. Prakash Vincent, Samuel Gnana Arun, Arumugaperumal Abdullah Al-Dhabi, Naif Valan Arasu, Mariadhas Young Kwon, Oh Kim, Young Ock Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title | Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title_full | Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title_fullStr | Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title_full_unstemmed | Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title_short | Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme by Bacillus sp. IND12 Using Response Surface Methodology in Solid-State Fermentation |
title_sort | novel sequential screening and enhanced production of fibrinolytic enzyme by bacillus sp. ind12 using response surface methodology in solid-state fermentation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5340989/ https://www.ncbi.nlm.nih.gov/pubmed/28321408 http://dx.doi.org/10.1155/2017/3909657 |
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