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Efficient and reliable establishment of lymphoblastoid cell lines by Epstein-Barr virus transformation from a limited amount of peripheral blood

Lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV) serve as an unlimited resource of human genomic DNA. The protocol that is widely used to establish LCLs involves peripheral blood mononuclear cell isolation by density gradient centrifugation, however, that method requires as m...

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Detalles Bibliográficos
Autores principales:	Omi, Natsue, Tokuda, Yuichi, Ikeda, Yoko, Ueno, Morio, Mori, Kazuhiko, Sotozono, Chie, Kinoshita, Shigeru, Nakano, Masakazu, Tashiro, Kei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341036/ https://www.ncbi.nlm.nih.gov/pubmed/28272413 http://dx.doi.org/10.1038/srep43833

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