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Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics
Soluble αβ-tubulin heterodimers are maintained at high concentration inside eukaryotic cells, forming pools that fundamentally drive microtubule dynamics. Five conserved tubulin cofactors and ADP ribosylation factor–like 2 regulate the biogenesis and degradation of αβ-tubulins to maintain concentrat...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341719/ https://www.ncbi.nlm.nih.gov/pubmed/28137948 http://dx.doi.org/10.1091/mbc.E15-10-0694 |
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author | Al-Bassam, Jawdat |
author_facet | Al-Bassam, Jawdat |
author_sort | Al-Bassam, Jawdat |
collection | PubMed |
description | Soluble αβ-tubulin heterodimers are maintained at high concentration inside eukaryotic cells, forming pools that fundamentally drive microtubule dynamics. Five conserved tubulin cofactors and ADP ribosylation factor–like 2 regulate the biogenesis and degradation of αβ-tubulins to maintain concentrated soluble pools. Here I describe a revised model for the function of three tubulin cofactors and Arl2 as a multisubunit GTP-hydrolyzing catalytic chaperone that cycles to promote αβ-tubulin biogenesis and degradation. This model helps explain old and new data indicating these activities enhance microtubule dynamics in vivo via repair or removal of αβ-tubulins from the soluble pools |
format | Online Article Text |
id | pubmed-5341719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53417192017-04-16 Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics Al-Bassam, Jawdat Mol Biol Cell Perspectives Soluble αβ-tubulin heterodimers are maintained at high concentration inside eukaryotic cells, forming pools that fundamentally drive microtubule dynamics. Five conserved tubulin cofactors and ADP ribosylation factor–like 2 regulate the biogenesis and degradation of αβ-tubulins to maintain concentrated soluble pools. Here I describe a revised model for the function of three tubulin cofactors and Arl2 as a multisubunit GTP-hydrolyzing catalytic chaperone that cycles to promote αβ-tubulin biogenesis and degradation. This model helps explain old and new data indicating these activities enhance microtubule dynamics in vivo via repair or removal of αβ-tubulins from the soluble pools The American Society for Cell Biology 2017-02-01 /pmc/articles/PMC5341719/ /pubmed/28137948 http://dx.doi.org/10.1091/mbc.E15-10-0694 Text en © 2017 Al-Bassam. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Perspectives Al-Bassam, Jawdat Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title | Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title_full | Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title_fullStr | Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title_full_unstemmed | Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title_short | Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
title_sort | revisiting the tubulin cofactors and arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics |
topic | Perspectives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341719/ https://www.ncbi.nlm.nih.gov/pubmed/28137948 http://dx.doi.org/10.1091/mbc.E15-10-0694 |
work_keys_str_mv | AT albassamjawdat revisitingthetubulincofactorsandarl2intheregulationofsolubleabtubulinpoolsandtheireffectonmicrotubuledynamics |