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Lipid droplets are central organelles for meiosis II progression during yeast sporulation
Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly invo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341727/ https://www.ncbi.nlm.nih.gov/pubmed/27932491 http://dx.doi.org/10.1091/mbc.E16-06-0375 |
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author | Hsu, Tzu-Han Chen, Rey-Huei Cheng, Yun-Hsin Wang, Chao-Wen |
author_facet | Hsu, Tzu-Han Chen, Rey-Huei Cheng, Yun-Hsin Wang, Chao-Wen |
author_sort | Hsu, Tzu-Han |
collection | PubMed |
description | Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly involving LDs and their contents. In this study, we provide cytological evidence that LDs form direct contacts with the prospore membrane (PSM) that is synthesized de novo during meiosis II to sequester the dividing nuclei in sporulating yeast. Lipidomic analyses indicate that TAG lipolysis releases free fatty acids at a time that correlates well with meiosis II progression, concomitant with phospholipid remodeling. Mutants lacking TAG or impaired of TAG hydrolysis show spore wall assembly defects, supporting a role for TAG and/or its metabolites in spore wall morphogenesis. Not only does LD integrity influence spore wall assembly, LDs are also essential for other aspects of spore development. Yeast cells lacking LDs are severely defective in PSM growth and organization and display disrupted spindles, producing dead spores or even failing to form spores. Together these results link LD physiology directly to a unique membrane morphogenesis process critical for development. |
format | Online Article Text |
id | pubmed-5341727 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53417272017-04-16 Lipid droplets are central organelles for meiosis II progression during yeast sporulation Hsu, Tzu-Han Chen, Rey-Huei Cheng, Yun-Hsin Wang, Chao-Wen Mol Biol Cell Articles Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly involving LDs and their contents. In this study, we provide cytological evidence that LDs form direct contacts with the prospore membrane (PSM) that is synthesized de novo during meiosis II to sequester the dividing nuclei in sporulating yeast. Lipidomic analyses indicate that TAG lipolysis releases free fatty acids at a time that correlates well with meiosis II progression, concomitant with phospholipid remodeling. Mutants lacking TAG or impaired of TAG hydrolysis show spore wall assembly defects, supporting a role for TAG and/or its metabolites in spore wall morphogenesis. Not only does LD integrity influence spore wall assembly, LDs are also essential for other aspects of spore development. Yeast cells lacking LDs are severely defective in PSM growth and organization and display disrupted spindles, producing dead spores or even failing to form spores. Together these results link LD physiology directly to a unique membrane morphogenesis process critical for development. The American Society for Cell Biology 2017-02-01 /pmc/articles/PMC5341727/ /pubmed/27932491 http://dx.doi.org/10.1091/mbc.E16-06-0375 Text en © 2017 Hsu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Hsu, Tzu-Han Chen, Rey-Huei Cheng, Yun-Hsin Wang, Chao-Wen Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title | Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title_full | Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title_fullStr | Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title_full_unstemmed | Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title_short | Lipid droplets are central organelles for meiosis II progression during yeast sporulation |
title_sort | lipid droplets are central organelles for meiosis ii progression during yeast sporulation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341727/ https://www.ncbi.nlm.nih.gov/pubmed/27932491 http://dx.doi.org/10.1091/mbc.E16-06-0375 |
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