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Lipid droplets are central organelles for meiosis II progression during yeast sporulation

Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly invo...

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Autores principales: Hsu, Tzu-Han, Chen, Rey-Huei, Cheng, Yun-Hsin, Wang, Chao-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341727/
https://www.ncbi.nlm.nih.gov/pubmed/27932491
http://dx.doi.org/10.1091/mbc.E16-06-0375
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author Hsu, Tzu-Han
Chen, Rey-Huei
Cheng, Yun-Hsin
Wang, Chao-Wen
author_facet Hsu, Tzu-Han
Chen, Rey-Huei
Cheng, Yun-Hsin
Wang, Chao-Wen
author_sort Hsu, Tzu-Han
collection PubMed
description Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly involving LDs and their contents. In this study, we provide cytological evidence that LDs form direct contacts with the prospore membrane (PSM) that is synthesized de novo during meiosis II to sequester the dividing nuclei in sporulating yeast. Lipidomic analyses indicate that TAG lipolysis releases free fatty acids at a time that correlates well with meiosis II progression, concomitant with phospholipid remodeling. Mutants lacking TAG or impaired of TAG hydrolysis show spore wall assembly defects, supporting a role for TAG and/or its metabolites in spore wall morphogenesis. Not only does LD integrity influence spore wall assembly, LDs are also essential for other aspects of spore development. Yeast cells lacking LDs are severely defective in PSM growth and organization and display disrupted spindles, producing dead spores or even failing to form spores. Together these results link LD physiology directly to a unique membrane morphogenesis process critical for development.
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spelling pubmed-53417272017-04-16 Lipid droplets are central organelles for meiosis II progression during yeast sporulation Hsu, Tzu-Han Chen, Rey-Huei Cheng, Yun-Hsin Wang, Chao-Wen Mol Biol Cell Articles Neutral lipids, predominantly triacylglycerol (TAG) and sterol ester, are stored within the cellular organelles termed lipid droplets (LDs). Although it is believed that the major function of LDs is to supply the cell with energy and membranes, little is known about the cellular events directly involving LDs and their contents. In this study, we provide cytological evidence that LDs form direct contacts with the prospore membrane (PSM) that is synthesized de novo during meiosis II to sequester the dividing nuclei in sporulating yeast. Lipidomic analyses indicate that TAG lipolysis releases free fatty acids at a time that correlates well with meiosis II progression, concomitant with phospholipid remodeling. Mutants lacking TAG or impaired of TAG hydrolysis show spore wall assembly defects, supporting a role for TAG and/or its metabolites in spore wall morphogenesis. Not only does LD integrity influence spore wall assembly, LDs are also essential for other aspects of spore development. Yeast cells lacking LDs are severely defective in PSM growth and organization and display disrupted spindles, producing dead spores or even failing to form spores. Together these results link LD physiology directly to a unique membrane morphogenesis process critical for development. The American Society for Cell Biology 2017-02-01 /pmc/articles/PMC5341727/ /pubmed/27932491 http://dx.doi.org/10.1091/mbc.E16-06-0375 Text en © 2017 Hsu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Hsu, Tzu-Han
Chen, Rey-Huei
Cheng, Yun-Hsin
Wang, Chao-Wen
Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title_full Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title_fullStr Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title_full_unstemmed Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title_short Lipid droplets are central organelles for meiosis II progression during yeast sporulation
title_sort lipid droplets are central organelles for meiosis ii progression during yeast sporulation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341727/
https://www.ncbi.nlm.nih.gov/pubmed/27932491
http://dx.doi.org/10.1091/mbc.E16-06-0375
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