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An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques

BACKGROUND: New interventions for tuberculosis are urgently needed. Non-human primate (NHP) models provide the most relevant pre-clinical models of human disease and play a critical role in vaccine development. Models utilising Asian cynomolgus macaque populations are well established but the restri...

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Autores principales: Sharpe, S. A., White, A. D., Sibley, L., Gleeson, F., Hall, G. A., Basaraba, R. J., McIntyre, A., Clark, S. O., Gooch, K., Marsh, P. D., Williams, A., Dennis, M. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342172/
https://www.ncbi.nlm.nih.gov/pubmed/28273087
http://dx.doi.org/10.1371/journal.pone.0171906
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author Sharpe, S. A.
White, A. D.
Sibley, L.
Gleeson, F.
Hall, G. A.
Basaraba, R. J.
McIntyre, A.
Clark, S. O.
Gooch, K.
Marsh, P. D.
Williams, A.
Dennis, M. J.
author_facet Sharpe, S. A.
White, A. D.
Sibley, L.
Gleeson, F.
Hall, G. A.
Basaraba, R. J.
McIntyre, A.
Clark, S. O.
Gooch, K.
Marsh, P. D.
Williams, A.
Dennis, M. J.
author_sort Sharpe, S. A.
collection PubMed
description BACKGROUND: New interventions for tuberculosis are urgently needed. Non-human primate (NHP) models provide the most relevant pre-clinical models of human disease and play a critical role in vaccine development. Models utilising Asian cynomolgus macaque populations are well established but the restricted genetic diversity of the Mauritian cynomolgus macaques may be of added value. METHODS: Mauritian cynomolgus macaques were exposed to a range of doses of M. tuberculosis delivered by aerosol, and the outcome was assessed using clinical, imaging and pathology-based measures. RESULTS: All macaques developed characteristic clinical signs and disease features of tuberculosis (TB). Disease burden and the ability to control disease were dependent on exposure dose. Mauritian cynomolgus macaques showed less variation in pulmonary disease burden and total gross pathology scores within exposure dose groups than either Indian rhesus macaques or Chinese cynomolgus macaques CONCLUSIONS: The genetic homogeneity of Mauritian cynomolgus macaques makes them a potentially useful model of human tuberculosis.
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spelling pubmed-53421722017-03-29 An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques Sharpe, S. A. White, A. D. Sibley, L. Gleeson, F. Hall, G. A. Basaraba, R. J. McIntyre, A. Clark, S. O. Gooch, K. Marsh, P. D. Williams, A. Dennis, M. J. PLoS One Research Article BACKGROUND: New interventions for tuberculosis are urgently needed. Non-human primate (NHP) models provide the most relevant pre-clinical models of human disease and play a critical role in vaccine development. Models utilising Asian cynomolgus macaque populations are well established but the restricted genetic diversity of the Mauritian cynomolgus macaques may be of added value. METHODS: Mauritian cynomolgus macaques were exposed to a range of doses of M. tuberculosis delivered by aerosol, and the outcome was assessed using clinical, imaging and pathology-based measures. RESULTS: All macaques developed characteristic clinical signs and disease features of tuberculosis (TB). Disease burden and the ability to control disease were dependent on exposure dose. Mauritian cynomolgus macaques showed less variation in pulmonary disease burden and total gross pathology scores within exposure dose groups than either Indian rhesus macaques or Chinese cynomolgus macaques CONCLUSIONS: The genetic homogeneity of Mauritian cynomolgus macaques makes them a potentially useful model of human tuberculosis. Public Library of Science 2017-03-08 /pmc/articles/PMC5342172/ /pubmed/28273087 http://dx.doi.org/10.1371/journal.pone.0171906 Text en © 2017 Sharpe et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sharpe, S. A.
White, A. D.
Sibley, L.
Gleeson, F.
Hall, G. A.
Basaraba, R. J.
McIntyre, A.
Clark, S. O.
Gooch, K.
Marsh, P. D.
Williams, A.
Dennis, M. J.
An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title_full An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title_fullStr An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title_full_unstemmed An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title_short An aerosol challenge model of tuberculosis in Mauritian cynomolgus macaques
title_sort aerosol challenge model of tuberculosis in mauritian cynomolgus macaques
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342172/
https://www.ncbi.nlm.nih.gov/pubmed/28273087
http://dx.doi.org/10.1371/journal.pone.0171906
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