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Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases

This study aimed to evaluate the diagnostic utilities of the automated connective tissues disease screening assay, CTD screen, in patients with systemic rheumatic diseases. A total of 1093 serum samples were assayed using CTD screen and indirect immunofluorescent (IIF) methods. Among them, 162 were...

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Autores principales: Jeong, Seri, Yang, Heeyoung, Hwang, Hyunyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342238/
https://www.ncbi.nlm.nih.gov/pubmed/28273146
http://dx.doi.org/10.1371/journal.pone.0173597
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author Jeong, Seri
Yang, Heeyoung
Hwang, Hyunyong
author_facet Jeong, Seri
Yang, Heeyoung
Hwang, Hyunyong
author_sort Jeong, Seri
collection PubMed
description This study aimed to evaluate the diagnostic utilities of the automated connective tissues disease screening assay, CTD screen, in patients with systemic rheumatic diseases. A total of 1093 serum samples were assayed using CTD screen and indirect immunofluorescent (IIF) methods. Among them, 162 were diagnosed with systemic rheumatic disease, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and mixed connective tissue disease (MCT). The remaining 931 with non-systemic rheumatic disease were assigned to the control group. The median ratios of CTD screen tests were significantly higher in the systemic rheumatic disease group than in the control group. The positive likelihood ratios of the CTD screen were higher than those of IIF in patients with total rheumatic diseases (4.1 vs. 1.6), including SLE (24.3 vs. 10.7). The areas under the receiver operating characteristic curves (ROC-AUCs) of the CTD screen for discriminating total rheumatic diseases, RA, SLE, and MCT from controls were 0.68, 0.56, 0.92 and 0.80, respectively. The ROC-AUCs of the combinations with IIF were significantly higher in patients with total rheumatic diseases (0.72) and MCT (0.85) than in those of the CTD screen alone. Multivariate analysis indicated that both the CTD screen and IIF were independent variables for predicting systemic rheumatic disease. CTD screen alone and in combination with IIF were a valuable diagnostic tool for predicting systemic rheumatic diseases, particularly for SLE.
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spelling pubmed-53422382017-03-29 Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases Jeong, Seri Yang, Heeyoung Hwang, Hyunyong PLoS One Research Article This study aimed to evaluate the diagnostic utilities of the automated connective tissues disease screening assay, CTD screen, in patients with systemic rheumatic diseases. A total of 1093 serum samples were assayed using CTD screen and indirect immunofluorescent (IIF) methods. Among them, 162 were diagnosed with systemic rheumatic disease, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and mixed connective tissue disease (MCT). The remaining 931 with non-systemic rheumatic disease were assigned to the control group. The median ratios of CTD screen tests were significantly higher in the systemic rheumatic disease group than in the control group. The positive likelihood ratios of the CTD screen were higher than those of IIF in patients with total rheumatic diseases (4.1 vs. 1.6), including SLE (24.3 vs. 10.7). The areas under the receiver operating characteristic curves (ROC-AUCs) of the CTD screen for discriminating total rheumatic diseases, RA, SLE, and MCT from controls were 0.68, 0.56, 0.92 and 0.80, respectively. The ROC-AUCs of the combinations with IIF were significantly higher in patients with total rheumatic diseases (0.72) and MCT (0.85) than in those of the CTD screen alone. Multivariate analysis indicated that both the CTD screen and IIF were independent variables for predicting systemic rheumatic disease. CTD screen alone and in combination with IIF were a valuable diagnostic tool for predicting systemic rheumatic diseases, particularly for SLE. Public Library of Science 2017-03-08 /pmc/articles/PMC5342238/ /pubmed/28273146 http://dx.doi.org/10.1371/journal.pone.0173597 Text en © 2017 Jeong et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jeong, Seri
Yang, Heeyoung
Hwang, Hyunyong
Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title_full Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title_fullStr Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title_full_unstemmed Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title_short Evaluation of an automated connective tissue disease screening assay in Korean patients with systemic rheumatic diseases
title_sort evaluation of an automated connective tissue disease screening assay in korean patients with systemic rheumatic diseases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342238/
https://www.ncbi.nlm.nih.gov/pubmed/28273146
http://dx.doi.org/10.1371/journal.pone.0173597
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