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p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer
In colorectal cancer, no study has been carried out discovering the relationship among p53, mitochondrial transcription factor A (TFAM) expression and change of mitochondrial DNA (mtDNA) copy number. In our study, co-expression of p53 and TFAM was observed in colon adenocarcinoma tissues, paracancer...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342792/ https://www.ncbi.nlm.nih.gov/pubmed/27732955 http://dx.doi.org/10.18632/oncotarget.12514 |
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author | Wen, Shilei Gao, Jinhang Zhang, Linhao Zhou, Hongying Fang, Dingzhi Feng, Shi |
author_facet | Wen, Shilei Gao, Jinhang Zhang, Linhao Zhou, Hongying Fang, Dingzhi Feng, Shi |
author_sort | Wen, Shilei |
collection | PubMed |
description | In colorectal cancer, no study has been carried out discovering the relationship among p53, mitochondrial transcription factor A (TFAM) expression and change of mitochondrial DNA (mtDNA) copy number. In our study, co-expression of p53 and TFAM was observed in colon adenocarcinoma tissues, paracancerous tissues and 9 colorectal cancer cell lines. Then, a significant linear correlation was established between either p53 or TFAM expression and advanced TNM stage, positive lymph nodes and low 5-year survival rate in patients with colon adenocarcinoma. Additionally, advanced TNM stage, large tumor burden, presence of distant metastasis, and high TFAM expression were significantly related to poor overall 5-years survival. Moreover, alteration of p53 expression could change TFAM expression but TFAM could not influence p53 expression, and p53 could enhance TFAM expression via binding to TFAM promoter. While, both of p53 and TFAM expression could incrase mtDNA copy number in vitro. In conclusions, p53 might incrase mtDNA copy number through its regulation on TFAM expression via TFAMpromoter. |
format | Online Article Text |
id | pubmed-5342792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53427922017-03-28 p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer Wen, Shilei Gao, Jinhang Zhang, Linhao Zhou, Hongying Fang, Dingzhi Feng, Shi Oncotarget Research Paper In colorectal cancer, no study has been carried out discovering the relationship among p53, mitochondrial transcription factor A (TFAM) expression and change of mitochondrial DNA (mtDNA) copy number. In our study, co-expression of p53 and TFAM was observed in colon adenocarcinoma tissues, paracancerous tissues and 9 colorectal cancer cell lines. Then, a significant linear correlation was established between either p53 or TFAM expression and advanced TNM stage, positive lymph nodes and low 5-year survival rate in patients with colon adenocarcinoma. Additionally, advanced TNM stage, large tumor burden, presence of distant metastasis, and high TFAM expression were significantly related to poor overall 5-years survival. Moreover, alteration of p53 expression could change TFAM expression but TFAM could not influence p53 expression, and p53 could enhance TFAM expression via binding to TFAM promoter. While, both of p53 and TFAM expression could incrase mtDNA copy number in vitro. In conclusions, p53 might incrase mtDNA copy number through its regulation on TFAM expression via TFAMpromoter. Impact Journals LLC 2016-10-07 /pmc/articles/PMC5342792/ /pubmed/27732955 http://dx.doi.org/10.18632/oncotarget.12514 Text en Copyright: © 2016 Wen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wen, Shilei Gao, Jinhang Zhang, Linhao Zhou, Hongying Fang, Dingzhi Feng, Shi p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title_full | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title_fullStr | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title_full_unstemmed | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title_short | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor A in colorectal cancer |
title_sort | p53 increase mitochondrial copy number via up-regulation of mitochondrial transcription factor a in colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342792/ https://www.ncbi.nlm.nih.gov/pubmed/27732955 http://dx.doi.org/10.18632/oncotarget.12514 |
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