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Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association

Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell s...

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Autores principales: Kim, Younghwa, Jeon, Yoon-Jae, Ryu, Kang, Kim, Tae-Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342871/
https://www.ncbi.nlm.nih.gov/pubmed/27881214
http://dx.doi.org/10.5483/BMBRep.2017.50.2.150
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author Kim, Younghwa
Jeon, Yoon-Jae
Ryu, Kang
Kim, Tae-Yoon
author_facet Kim, Younghwa
Jeon, Yoon-Jae
Ryu, Kang
Kim, Tae-Yoon
author_sort Kim, Younghwa
collection PubMed
description Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that Zn(2+) promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of Zn(2+). This resulted in increased inhibitory effects of rhSOD3 on NF-kB and STAT3 signals in the presence of Zn(2+), which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine.
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spelling pubmed-53428712017-04-20 Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association Kim, Younghwa Jeon, Yoon-Jae Ryu, Kang Kim, Tae-Yoon BMB Rep Articles Recently, we demonstrated that superoxide dismutase 3 (SOD3) is a strong candidate for biomedicine. Anti-oxidant function of SOD3 was accomplished without cell penetration, and it inhibited the inflammatory responses via non-enzymatic functions. SOD3 has the heparin binding domain associating cell surface. Interestingly, we found that Zn(2+) promotes transduction effects of recombinant human SOD3 (rhSOD3) by increasing uptake via the heparin binding domain (HBD). We demonstrated an uptake of rhSOD3 from media to cell lysate via HBD, resulting in an accumulation of rhSOD3 in the nucleus, which was promoted by the presence of Zn(2+). This resulted in increased inhibitory effects of rhSOD3 on NF-kB and STAT3 signals in the presence of Zn(2+), which shows elevated association of rhSOD3 into the cells. These results suggest that an optimized procedure can help to enhance the inflammatory efficacy of rhSOD3, as a novel biomedicine. Korean Society for Biochemistry and Molecular Biology 2017 2017-02-28 /pmc/articles/PMC5342871/ /pubmed/27881214 http://dx.doi.org/10.5483/BMBRep.2017.50.2.150 Text en Copyright © 2017 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Kim, Younghwa
Jeon, Yoon-Jae
Ryu, Kang
Kim, Tae-Yoon
Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title_full Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title_fullStr Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title_full_unstemmed Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title_short Zinc(II) ion promotes anti-inflammatory effects of rhSOD3 by increasing cellular association
title_sort zinc(ii) ion promotes anti-inflammatory effects of rhsod3 by increasing cellular association
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342871/
https://www.ncbi.nlm.nih.gov/pubmed/27881214
http://dx.doi.org/10.5483/BMBRep.2017.50.2.150
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