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Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity
Quercetin, a plant-derived flavonoid found in fruits, vegetables and tea, has been known to possess bioactive properties such as anti-oxidant, anti-inflammatory and anti-cancer. In this study, anti-cancer effect of quercetin and its underlying mechanisms in triple-negative breast cancer cells was in...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Physiological Society and The Korean Society of Pharmacology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343054/ https://www.ncbi.nlm.nih.gov/pubmed/28280414 http://dx.doi.org/10.4196/kjpp.2017.21.2.205 |
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author | Nguyen, Lich Thi Lee, Yeon-Hee Sharma, Ashish Ranjan Park, Jong-Bong Jagga, Supriya Sharma, Garima Lee, Sang-Soo Nam, Ju-Suk |
author_facet | Nguyen, Lich Thi Lee, Yeon-Hee Sharma, Ashish Ranjan Park, Jong-Bong Jagga, Supriya Sharma, Garima Lee, Sang-Soo Nam, Ju-Suk |
author_sort | Nguyen, Lich Thi |
collection | PubMed |
description | Quercetin, a plant-derived flavonoid found in fruits, vegetables and tea, has been known to possess bioactive properties such as anti-oxidant, anti-inflammatory and anti-cancer. In this study, anti-cancer effect of quercetin and its underlying mechanisms in triple-negative breast cancer cells was investigated. MTT assay showed that quercetin reduced breast cancer cell viability in a time and dose dependent manner. For this, quercetin not only increased cell apoptosis but also inhibited cell cycle progression. Moreover, quercetin increased FasL mRNA expression and p51, p21 and GADD45 signaling activities. We also observed that quercetin induced protein level, transcriptional activity and nuclear translocation of Foxo3a. Knockdown of Foxo3a caused significant reduction in the effect of quercetin on cell apoptosis and cell cycle arrest. In addition, treatment of JNK inhibitor (SP 600125) abolished quercetin-stimulated Foxo3a activity, suggesting JNK as a possible upstream signaling in regulation of Foxo3a activity. Knockdown of Foxo3a and inhibition of JNK activity reduced the signaling activities of p53, p21 and GADD45, triggered by quercetin. Taken together, our study suggests that quercetin induces apoptosis and cell cycle arrest via modification of Foxo3a signaling in triple-negative breast cancer cells. |
format | Online Article Text |
id | pubmed-5343054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53430542017-03-09 Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity Nguyen, Lich Thi Lee, Yeon-Hee Sharma, Ashish Ranjan Park, Jong-Bong Jagga, Supriya Sharma, Garima Lee, Sang-Soo Nam, Ju-Suk Korean J Physiol Pharmacol Original Article Quercetin, a plant-derived flavonoid found in fruits, vegetables and tea, has been known to possess bioactive properties such as anti-oxidant, anti-inflammatory and anti-cancer. In this study, anti-cancer effect of quercetin and its underlying mechanisms in triple-negative breast cancer cells was investigated. MTT assay showed that quercetin reduced breast cancer cell viability in a time and dose dependent manner. For this, quercetin not only increased cell apoptosis but also inhibited cell cycle progression. Moreover, quercetin increased FasL mRNA expression and p51, p21 and GADD45 signaling activities. We also observed that quercetin induced protein level, transcriptional activity and nuclear translocation of Foxo3a. Knockdown of Foxo3a caused significant reduction in the effect of quercetin on cell apoptosis and cell cycle arrest. In addition, treatment of JNK inhibitor (SP 600125) abolished quercetin-stimulated Foxo3a activity, suggesting JNK as a possible upstream signaling in regulation of Foxo3a activity. Knockdown of Foxo3a and inhibition of JNK activity reduced the signaling activities of p53, p21 and GADD45, triggered by quercetin. Taken together, our study suggests that quercetin induces apoptosis and cell cycle arrest via modification of Foxo3a signaling in triple-negative breast cancer cells. The Korean Physiological Society and The Korean Society of Pharmacology 2017-03 2017-02-21 /pmc/articles/PMC5343054/ /pubmed/28280414 http://dx.doi.org/10.4196/kjpp.2017.21.2.205 Text en Copyright © Korean J Physiol Pharmacol http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nguyen, Lich Thi Lee, Yeon-Hee Sharma, Ashish Ranjan Park, Jong-Bong Jagga, Supriya Sharma, Garima Lee, Sang-Soo Nam, Ju-Suk Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title | Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title_full | Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title_fullStr | Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title_full_unstemmed | Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title_short | Quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of Foxo3a activity |
title_sort | quercetin induces apoptosis and cell cycle arrest in triple-negative breast cancer cells through modulation of foxo3a activity |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343054/ https://www.ncbi.nlm.nih.gov/pubmed/28280414 http://dx.doi.org/10.4196/kjpp.2017.21.2.205 |
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