Cargando…

Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) represent a potential unlimited cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, hPSC-CMs produced by current protocols are not representative of native adult human cardiomyocytes as they...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Xuetao, Nunes, Sara S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343210/
https://www.ncbi.nlm.nih.gov/pubmed/28337437
http://dx.doi.org/10.3389/fcell.2017.00019
_version_ 1782513320069169152
author Sun, Xuetao
Nunes, Sara S.
author_facet Sun, Xuetao
Nunes, Sara S.
author_sort Sun, Xuetao
collection PubMed
description Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) represent a potential unlimited cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, hPSC-CMs produced by current protocols are not representative of native adult human cardiomyocytes as they display immature gene expression profile, structure and function. In order to improve hPSC-CM maturity and function, various approaches have been developed, including genetic manipulations to induce gene expression, delivery of biochemical factors, such as triiodothyronine and alpha-adrenergic agonist phenylephrine, induction of cell alignment in 3D tissues, mechanical stress as a mimic of cardiac load and electrical stimulation/pacing or a combination of these. In this mini review, we discuss biomimetic strategies for the maturation for hPSC-CMs with a particular focus on electromechanical conditioning methods.
format Online
Article
Text
id pubmed-5343210
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-53432102017-03-23 Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes Sun, Xuetao Nunes, Sara S. Front Cell Dev Biol Cell and Developmental Biology Human pluripotent stem cell-derived cardiomyocytes (hPSC-CM) represent a potential unlimited cell supply for cardiac tissue engineering and possibly regenerative medicine applications. However, hPSC-CMs produced by current protocols are not representative of native adult human cardiomyocytes as they display immature gene expression profile, structure and function. In order to improve hPSC-CM maturity and function, various approaches have been developed, including genetic manipulations to induce gene expression, delivery of biochemical factors, such as triiodothyronine and alpha-adrenergic agonist phenylephrine, induction of cell alignment in 3D tissues, mechanical stress as a mimic of cardiac load and electrical stimulation/pacing or a combination of these. In this mini review, we discuss biomimetic strategies for the maturation for hPSC-CMs with a particular focus on electromechanical conditioning methods. Frontiers Media S.A. 2017-03-09 /pmc/articles/PMC5343210/ /pubmed/28337437 http://dx.doi.org/10.3389/fcell.2017.00019 Text en Copyright © 2017 Sun and Nunes. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Sun, Xuetao
Nunes, Sara S.
Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title_full Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title_fullStr Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title_full_unstemmed Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title_short Bioengineering Approaches to Mature Human Pluripotent Stem Cell-Derived Cardiomyocytes
title_sort bioengineering approaches to mature human pluripotent stem cell-derived cardiomyocytes
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343210/
https://www.ncbi.nlm.nih.gov/pubmed/28337437
http://dx.doi.org/10.3389/fcell.2017.00019
work_keys_str_mv AT sunxuetao bioengineeringapproachestomaturehumanpluripotentstemcellderivedcardiomyocytes
AT nunessaras bioengineeringapproachestomaturehumanpluripotentstemcellderivedcardiomyocytes