Cargando…

Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves

BACKGROUND: Bacterial endocarditis is a recognised disease in humans and animals. In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. Endocarditis in cattle is a common post-mortem finding in abattoirs and studies have identif...

Descripción completa

Detalles Bibliográficos
Autores principales: Agerholm, Jørgen S., Jensen, Tim K., Agger, Jens F., Engelsma, Marc Y., Roest, Hendrik I. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343293/
https://www.ncbi.nlm.nih.gov/pubmed/28274243
http://dx.doi.org/10.1186/s12917-017-0988-5
_version_ 1782513338472726528
author Agerholm, Jørgen S.
Jensen, Tim K.
Agger, Jens F.
Engelsma, Marc Y.
Roest, Hendrik I. J.
author_facet Agerholm, Jørgen S.
Jensen, Tim K.
Agger, Jens F.
Engelsma, Marc Y.
Roest, Hendrik I. J.
author_sort Agerholm, Jørgen S.
collection PubMed
description BACKGROUND: Bacterial endocarditis is a recognised disease in humans and animals. In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. Endocarditis in cattle is a common post-mortem finding in abattoirs and studies have identified Trueperella pyogenes as a major cause. Despite exposure of cattle to C. burnetii, the significance of this particular bacterium for development and progression of endocarditis has not been studied in detail. Cardiac valves of cattle affected with endocarditis (n = 100) were examined by histology, fluorescence in situ hybridization (FISH) and real time quantitative polymerase chain reaction (PCR). Serum was examined for anti-C. burnetii antibodies by enzyme-linked immunosorbent assay (ELISA). RESULTS: Serology revealed that 70% of the cattle were positive for antibodies to C. burnetii, while PCR analysis identified 25% of endocarditis valve samples as being positive. C. burnetii was not detected by FISH, probably due to the low infection levels. Most cattle had chronic valvular vegetative endocarditis with lesions being characterised by a core of fibrous tissue covered by significant amounts of fibrin, sometimes with areas of liquefaction, and with a coagulum covering the surface. In a few cases, including the case with the highest infection level, lesions were characterized by extensive fibrosis and calcification. Histologically, bacteria other than C. burnetii were observed in most cases. CONCLUSIONS: The presence of C. burnetii DNA is relatively common in cattle affected with valvular endocarditis. The role of C. burnetii remains however unknown as lesions did not differ between C. burnetii infected and non-infected cattle and because T. pyogenes–like bacteria were present in the inflamed valves; a bacterium able to induce the observed lesions. Heart valves of normal cattle should be investigated to assess if C. burnetii may be present without preexisting lesions.
format Online
Article
Text
id pubmed-5343293
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-53432932017-03-10 Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves Agerholm, Jørgen S. Jensen, Tim K. Agger, Jens F. Engelsma, Marc Y. Roest, Hendrik I. J. BMC Vet Res Research Article BACKGROUND: Bacterial endocarditis is a recognised disease in humans and animals. In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. Endocarditis in cattle is a common post-mortem finding in abattoirs and studies have identified Trueperella pyogenes as a major cause. Despite exposure of cattle to C. burnetii, the significance of this particular bacterium for development and progression of endocarditis has not been studied in detail. Cardiac valves of cattle affected with endocarditis (n = 100) were examined by histology, fluorescence in situ hybridization (FISH) and real time quantitative polymerase chain reaction (PCR). Serum was examined for anti-C. burnetii antibodies by enzyme-linked immunosorbent assay (ELISA). RESULTS: Serology revealed that 70% of the cattle were positive for antibodies to C. burnetii, while PCR analysis identified 25% of endocarditis valve samples as being positive. C. burnetii was not detected by FISH, probably due to the low infection levels. Most cattle had chronic valvular vegetative endocarditis with lesions being characterised by a core of fibrous tissue covered by significant amounts of fibrin, sometimes with areas of liquefaction, and with a coagulum covering the surface. In a few cases, including the case with the highest infection level, lesions were characterized by extensive fibrosis and calcification. Histologically, bacteria other than C. burnetii were observed in most cases. CONCLUSIONS: The presence of C. burnetii DNA is relatively common in cattle affected with valvular endocarditis. The role of C. burnetii remains however unknown as lesions did not differ between C. burnetii infected and non-infected cattle and because T. pyogenes–like bacteria were present in the inflamed valves; a bacterium able to induce the observed lesions. Heart valves of normal cattle should be investigated to assess if C. burnetii may be present without preexisting lesions. BioMed Central 2017-03-09 /pmc/articles/PMC5343293/ /pubmed/28274243 http://dx.doi.org/10.1186/s12917-017-0988-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Agerholm, Jørgen S.
Jensen, Tim K.
Agger, Jens F.
Engelsma, Marc Y.
Roest, Hendrik I. J.
Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title_full Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title_fullStr Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title_full_unstemmed Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title_short Presence of Coxiella burnetii DNA in inflamed bovine cardiac valves
title_sort presence of coxiella burnetii dna in inflamed bovine cardiac valves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343293/
https://www.ncbi.nlm.nih.gov/pubmed/28274243
http://dx.doi.org/10.1186/s12917-017-0988-5
work_keys_str_mv AT agerholmjørgens presenceofcoxiellaburnetiidnaininflamedbovinecardiacvalves
AT jensentimk presenceofcoxiellaburnetiidnaininflamedbovinecardiacvalves
AT aggerjensf presenceofcoxiellaburnetiidnaininflamedbovinecardiacvalves
AT engelsmamarcy presenceofcoxiellaburnetiidnaininflamedbovinecardiacvalves
AT roesthendrikij presenceofcoxiellaburnetiidnaininflamedbovinecardiacvalves