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Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato
BACKGROUND: Late blight, caused by oomycetes pathogen Phytophthora infestans (Mont.) de Bary, is the most devastating potato disease in the world. RB gene from Solanum bulbocastanum has been shown to impart broad spectrum resistance against P. infestans races. In this study Katahdin transgenic event...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343411/ https://www.ncbi.nlm.nih.gov/pubmed/28274218 http://dx.doi.org/10.1186/s12863-017-0490-x |
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author | Shandil, Rajesh K. Chakrabarti, Swarup K. Singh, Bir Pal Sharma, Sanjeev Sundaresha, S. Kaushik, Surinder K. Bhatt, Arvind K. Sharma, Nitya Nand |
author_facet | Shandil, Rajesh K. Chakrabarti, Swarup K. Singh, Bir Pal Sharma, Sanjeev Sundaresha, S. Kaushik, Surinder K. Bhatt, Arvind K. Sharma, Nitya Nand |
author_sort | Shandil, Rajesh K. |
collection | PubMed |
description | BACKGROUND: Late blight, caused by oomycetes pathogen Phytophthora infestans (Mont.) de Bary, is the most devastating potato disease in the world. RB gene from Solanum bulbocastanum has been shown to impart broad spectrum resistance against P. infestans races. In this study Katahdin transgenic event SP951 was used as male parent to cross with the popular Indian potato cultivars viz., Kufri Bahar (KB) and Kufri Jyoti (KJ) to enhance the late blight resistance. RESULTS: Populations of 271 F1seedlings from the crosses KB × SP951 (87) and KJ × SP951 (184) were screened for inheritance of RB transgene through PCR and bioassay. Disease response based on AUDPC of different hybrid lines varied from immunity to complete susceptibility. High degree of resistance (<25% infection) was observed in KJ × SP951 derived seedlings (85.2%), whereas level of resistance in KB × SP951 (36.4% infection) derived seedlings was of low order. CONCLUSION: This study provides valuable genetic materials for development of potentially durable late blight resistant potato varieties. Besides, it also corroborates the fact that efficacy of R gene is not solely dependent on its presence in the variety but largely depends on the genetic background of the recipient genotype. |
format | Online Article Text |
id | pubmed-5343411 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53434112017-03-10 Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato Shandil, Rajesh K. Chakrabarti, Swarup K. Singh, Bir Pal Sharma, Sanjeev Sundaresha, S. Kaushik, Surinder K. Bhatt, Arvind K. Sharma, Nitya Nand BMC Genet Research Article BACKGROUND: Late blight, caused by oomycetes pathogen Phytophthora infestans (Mont.) de Bary, is the most devastating potato disease in the world. RB gene from Solanum bulbocastanum has been shown to impart broad spectrum resistance against P. infestans races. In this study Katahdin transgenic event SP951 was used as male parent to cross with the popular Indian potato cultivars viz., Kufri Bahar (KB) and Kufri Jyoti (KJ) to enhance the late blight resistance. RESULTS: Populations of 271 F1seedlings from the crosses KB × SP951 (87) and KJ × SP951 (184) were screened for inheritance of RB transgene through PCR and bioassay. Disease response based on AUDPC of different hybrid lines varied from immunity to complete susceptibility. High degree of resistance (<25% infection) was observed in KJ × SP951 derived seedlings (85.2%), whereas level of resistance in KB × SP951 (36.4% infection) derived seedlings was of low order. CONCLUSION: This study provides valuable genetic materials for development of potentially durable late blight resistant potato varieties. Besides, it also corroborates the fact that efficacy of R gene is not solely dependent on its presence in the variety but largely depends on the genetic background of the recipient genotype. BioMed Central 2017-03-09 /pmc/articles/PMC5343411/ /pubmed/28274218 http://dx.doi.org/10.1186/s12863-017-0490-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Shandil, Rajesh K. Chakrabarti, Swarup K. Singh, Bir Pal Sharma, Sanjeev Sundaresha, S. Kaushik, Surinder K. Bhatt, Arvind K. Sharma, Nitya Nand Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title | Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title_full | Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title_fullStr | Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title_full_unstemmed | Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title_short | Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato |
title_sort | genotypic background of the recipient plant is crucial for conferring rb gene mediated late blight resistance in potato |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343411/ https://www.ncbi.nlm.nih.gov/pubmed/28274218 http://dx.doi.org/10.1186/s12863-017-0490-x |
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