Cargando…

Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification

[Image: see text] To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the b...

Descripción completa

Detalles Bibliográficos
Autores principales: Bidulock, Allison C. E., Dubský, Pavel, van den Berg, Albert, Eijkel, Jan C. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343550/
https://www.ncbi.nlm.nih.gov/pubmed/28192985
http://dx.doi.org/10.1021/acs.analchem.6b04172
_version_ 1782513385526525952
author Bidulock, Allison C. E.
Dubský, Pavel
van den Berg, Albert
Eijkel, Jan C. T.
author_facet Bidulock, Allison C. E.
Dubský, Pavel
van den Berg, Albert
Eijkel, Jan C. T.
author_sort Bidulock, Allison C. E.
collection PubMed
description [Image: see text] To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample—thus eliminating the need for additional sample manipulation, microchip redesigns, and/or system expansions required for traditional ISTD usage. Cs and Li ions were added as integrated ISTDs to the BGE, and their effects on the reproducibility of Na quantification were explored. Results were then compared to the conclusions of our previous publication which used Cs and Li as traditional ISTDs. The in-house fabricated microchips, electrophoretic protocols, and solution matrixes were kept constant, allowing the proposed method to be reliably compared to the traditional method. Using the integrated ISTDs, both Cs and Li improved the Na peak area reproducibility approximately 2-fold, to final RSD values of 2.2–4.7% (n = 900). In contrast (to previous work), Cs as a traditional ISTD resulted in final RSDs of 2.5–8.8%, while the traditional Li ISTD performed poorly with RSDs of 6.3–14.2%. These findings suggest integrated ISTDs are a viable method to improve the precision of disposable MCE devices—giving matched or superior results to the traditional method in this study while neither increasing system cost nor complexity.
format Online
Article
Text
id pubmed-5343550
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-53435502017-03-10 Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification Bidulock, Allison C. E. Dubský, Pavel van den Berg, Albert Eijkel, Jan C. T. Anal Chem [Image: see text] To improve point-of-care quantification using microchip capillary electrophoresis (MCE), the chip-to-chip variabilities inherent in disposable, single-use devices must be addressed. This work proposes to integrate an internal standard (ISTD) into the microchip by adding it to the background electrolyte (BGE) instead of the sample—thus eliminating the need for additional sample manipulation, microchip redesigns, and/or system expansions required for traditional ISTD usage. Cs and Li ions were added as integrated ISTDs to the BGE, and their effects on the reproducibility of Na quantification were explored. Results were then compared to the conclusions of our previous publication which used Cs and Li as traditional ISTDs. The in-house fabricated microchips, electrophoretic protocols, and solution matrixes were kept constant, allowing the proposed method to be reliably compared to the traditional method. Using the integrated ISTDs, both Cs and Li improved the Na peak area reproducibility approximately 2-fold, to final RSD values of 2.2–4.7% (n = 900). In contrast (to previous work), Cs as a traditional ISTD resulted in final RSDs of 2.5–8.8%, while the traditional Li ISTD performed poorly with RSDs of 6.3–14.2%. These findings suggest integrated ISTDs are a viable method to improve the precision of disposable MCE devices—giving matched or superior results to the traditional method in this study while neither increasing system cost nor complexity. American Chemical Society 2017-02-02 2017-03-07 /pmc/articles/PMC5343550/ /pubmed/28192985 http://dx.doi.org/10.1021/acs.analchem.6b04172 Text en Copyright © 2017 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Bidulock, Allison C. E.
Dubský, Pavel
van den Berg, Albert
Eijkel, Jan C. T.
Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title_full Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title_fullStr Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title_full_unstemmed Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title_short Integrating Internal Standards into Disposable Capillary Electrophoresis Devices To Improve Quantification
title_sort integrating internal standards into disposable capillary electrophoresis devices to improve quantification
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343550/
https://www.ncbi.nlm.nih.gov/pubmed/28192985
http://dx.doi.org/10.1021/acs.analchem.6b04172
work_keys_str_mv AT bidulockallisonce integratinginternalstandardsintodisposablecapillaryelectrophoresisdevicestoimprovequantification
AT dubskypavel integratinginternalstandardsintodisposablecapillaryelectrophoresisdevicestoimprovequantification
AT vandenbergalbert integratinginternalstandardsintodisposablecapillaryelectrophoresisdevicestoimprovequantification
AT eijkeljanct integratinginternalstandardsintodisposablecapillaryelectrophoresisdevicestoimprovequantification