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Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa

Mammalian spermatozoa deliver various classes of RNAs to the oocyte during fertilization, and many of them may regulate fertility. The objective of the present study was to determine the composition and abundance of spermatozoal transcripts in fresh bull semen. The entire transcriptome of the sperma...

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Autores principales: Selvaraju, Sellappan, Parthipan, Sivashanmugam, Somashekar, Lakshminarayana, Kolte, Atul P, Krishnan Binsila, B., Arangasamy, Arunachalam, Ravindra, Janivara Parameshwaraiah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343582/
https://www.ncbi.nlm.nih.gov/pubmed/28276431
http://dx.doi.org/10.1038/srep42392
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author Selvaraju, Sellappan
Parthipan, Sivashanmugam
Somashekar, Lakshminarayana
Kolte, Atul P
Krishnan Binsila, B.
Arangasamy, Arunachalam
Ravindra, Janivara Parameshwaraiah
author_facet Selvaraju, Sellappan
Parthipan, Sivashanmugam
Somashekar, Lakshminarayana
Kolte, Atul P
Krishnan Binsila, B.
Arangasamy, Arunachalam
Ravindra, Janivara Parameshwaraiah
author_sort Selvaraju, Sellappan
collection PubMed
description Mammalian spermatozoa deliver various classes of RNAs to the oocyte during fertilization, and many of them may regulate fertility. The objective of the present study was to determine the composition and abundance of spermatozoal transcripts in fresh bull semen. The entire transcriptome of the spermatozoa from bulls (n = 3) was sequenced using two different platforms (Ion Proton and Illumina) to identify the maximum number of genes present in the spermatozoa. The bovine spermatozoa contained transcripts for 13,833 genes (transcripts per million, TPM > 10). Both intact and fragmented transcripts were found. These spermatozoal transcripts were associated with various stages of spermatogenesis, spermatozoal function, fertilization, and embryo development. The presence of intact transcripts of pregnancy-associated glycoproteins (PAGs) in the spermatozoa suggest a possible influence of sperm transcripts beyond early embryonic development. The specific regions (exon, intron, and exon-intron) of the particular spermatozoal transcripts might help regulate fertilization. This study demonstrates that the use of two different RNA-seq platforms provides a comprehensive profile of bovine spermatozoal RNA. Spermatozoal RNA profiling may be useful as a non-invasive method to delineate possible causes of male infertility and to predict fertility in a manner that is more effective than the conventional methods.
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spelling pubmed-53435822017-03-14 Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa Selvaraju, Sellappan Parthipan, Sivashanmugam Somashekar, Lakshminarayana Kolte, Atul P Krishnan Binsila, B. Arangasamy, Arunachalam Ravindra, Janivara Parameshwaraiah Sci Rep Article Mammalian spermatozoa deliver various classes of RNAs to the oocyte during fertilization, and many of them may regulate fertility. The objective of the present study was to determine the composition and abundance of spermatozoal transcripts in fresh bull semen. The entire transcriptome of the spermatozoa from bulls (n = 3) was sequenced using two different platforms (Ion Proton and Illumina) to identify the maximum number of genes present in the spermatozoa. The bovine spermatozoa contained transcripts for 13,833 genes (transcripts per million, TPM > 10). Both intact and fragmented transcripts were found. These spermatozoal transcripts were associated with various stages of spermatogenesis, spermatozoal function, fertilization, and embryo development. The presence of intact transcripts of pregnancy-associated glycoproteins (PAGs) in the spermatozoa suggest a possible influence of sperm transcripts beyond early embryonic development. The specific regions (exon, intron, and exon-intron) of the particular spermatozoal transcripts might help regulate fertilization. This study demonstrates that the use of two different RNA-seq platforms provides a comprehensive profile of bovine spermatozoal RNA. Spermatozoal RNA profiling may be useful as a non-invasive method to delineate possible causes of male infertility and to predict fertility in a manner that is more effective than the conventional methods. Nature Publishing Group 2017-03-09 /pmc/articles/PMC5343582/ /pubmed/28276431 http://dx.doi.org/10.1038/srep42392 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Selvaraju, Sellappan
Parthipan, Sivashanmugam
Somashekar, Lakshminarayana
Kolte, Atul P
Krishnan Binsila, B.
Arangasamy, Arunachalam
Ravindra, Janivara Parameshwaraiah
Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title_full Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title_fullStr Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title_full_unstemmed Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title_short Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa
title_sort occurrence and functional significance of the transcriptome in bovine (bos taurus) spermatozoa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343582/
https://www.ncbi.nlm.nih.gov/pubmed/28276431
http://dx.doi.org/10.1038/srep42392
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