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Building a 5-HT3A Receptor Expression Map in the Mouse Brain
Of the many serotonin receptors, the type 3 receptors (5-HT3R) are the only ionotropic ones, playing a key role in fast synaptic transmission and cognitive and emotional brain function through controlled neuronal excitation. To better understand the various functions of 5-HT3Rs, it is very important...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343592/ https://www.ncbi.nlm.nih.gov/pubmed/28276429 http://dx.doi.org/10.1038/srep42884 |
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author | Koyama, Yoshihisa Kondo, Makoto Shimada, Shoichi |
author_facet | Koyama, Yoshihisa Kondo, Makoto Shimada, Shoichi |
author_sort | Koyama, Yoshihisa |
collection | PubMed |
description | Of the many serotonin receptors, the type 3 receptors (5-HT3R) are the only ionotropic ones, playing a key role in fast synaptic transmission and cognitive and emotional brain function through controlled neuronal excitation. To better understand the various functions of 5-HT3Rs, it is very important to know their expression pattern in the central nervous system (CNS). To date, many distributional studies have shown localized 5-HT3R expression in the brain and spinal cord. However, an accurate pattern of 5-HT3R expression in the CNS remains to be elucidated. To investigate the distribution of 5-HT3R in the mouse brain in detail, we performed immunofluorescent staining using 5-HT3AR-GFP transgenic mice. We found strong 5-HT3AR expression in the olfactory bulb, cerebral cortex, hippocampus, and amygdala; and partial expression in the pons, medulla, and spinal cord. Meanwhile, the thalamus, hypothalamus, and midbrain exhibited a few 5-HT3AR-expressing cells, and no expression was detected in the cerebellum. Further, double-immunostaining using neural markers confirmed that 5-HT3AR is expressed in GABAergic interneurons containing somatostatin or calretinin. In the present study, we built a 5-HT3AR expression map in the mouse brain. Our findings make significant contributions in elucidating the novel functions of 5-HT3R in the CNS. |
format | Online Article Text |
id | pubmed-5343592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53435922017-03-14 Building a 5-HT3A Receptor Expression Map in the Mouse Brain Koyama, Yoshihisa Kondo, Makoto Shimada, Shoichi Sci Rep Article Of the many serotonin receptors, the type 3 receptors (5-HT3R) are the only ionotropic ones, playing a key role in fast synaptic transmission and cognitive and emotional brain function through controlled neuronal excitation. To better understand the various functions of 5-HT3Rs, it is very important to know their expression pattern in the central nervous system (CNS). To date, many distributional studies have shown localized 5-HT3R expression in the brain and spinal cord. However, an accurate pattern of 5-HT3R expression in the CNS remains to be elucidated. To investigate the distribution of 5-HT3R in the mouse brain in detail, we performed immunofluorescent staining using 5-HT3AR-GFP transgenic mice. We found strong 5-HT3AR expression in the olfactory bulb, cerebral cortex, hippocampus, and amygdala; and partial expression in the pons, medulla, and spinal cord. Meanwhile, the thalamus, hypothalamus, and midbrain exhibited a few 5-HT3AR-expressing cells, and no expression was detected in the cerebellum. Further, double-immunostaining using neural markers confirmed that 5-HT3AR is expressed in GABAergic interneurons containing somatostatin or calretinin. In the present study, we built a 5-HT3AR expression map in the mouse brain. Our findings make significant contributions in elucidating the novel functions of 5-HT3R in the CNS. Nature Publishing Group 2017-03-09 /pmc/articles/PMC5343592/ /pubmed/28276429 http://dx.doi.org/10.1038/srep42884 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Koyama, Yoshihisa Kondo, Makoto Shimada, Shoichi Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title | Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title_full | Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title_fullStr | Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title_full_unstemmed | Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title_short | Building a 5-HT3A Receptor Expression Map in the Mouse Brain |
title_sort | building a 5-ht3a receptor expression map in the mouse brain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343592/ https://www.ncbi.nlm.nih.gov/pubmed/28276429 http://dx.doi.org/10.1038/srep42884 |
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