Cargando…

Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study

Alteration of miRNAs and dietary polyunsaturated fatty acids (PUFAs) underlies vascular inflammation. PUFAs are known to be incorporated into the cell membrane of monocytes/macrophages or endothelial cells, the major cellular players of vascular diseases, thereby affecting cellular signal transducti...

Descripción completa

Detalles Bibliográficos
Autores principales: Roessler, Claudia, Kuhlmann, Kevin, Hellwing, Christine, Leimert, Anja, Schumann, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343820/
https://www.ncbi.nlm.nih.gov/pubmed/28134837
http://dx.doi.org/10.3390/ijms18020284
_version_ 1782513431257022464
author Roessler, Claudia
Kuhlmann, Kevin
Hellwing, Christine
Leimert, Anja
Schumann, Julia
author_facet Roessler, Claudia
Kuhlmann, Kevin
Hellwing, Christine
Leimert, Anja
Schumann, Julia
author_sort Roessler, Claudia
collection PubMed
description Alteration of miRNAs and dietary polyunsaturated fatty acids (PUFAs) underlies vascular inflammation. PUFAs are known to be incorporated into the cell membrane of monocytes/macrophages or endothelial cells, the major cellular players of vascular diseases, thereby affecting cellular signal transduction. Nevertheless, there are no investigations concerning the PUFA impact on miRNA expression by these cells. With regard to the key role miRNAs play for overall cellular functionality, this study aims to elucidate whether PUFAs affect miRNA expression profiles. To this end, the monocyte/macrophage cell line RAW264.7 and the endothelial cell line TIME were enriched with either docosahexaenoic acid (DHA; n3-PUFA) or arachidonic acid (AA; n6-PUFA) until reaching a stable incorporation into the plasma membrane and, at least in part, exposed to an inflammatory milieu. Expressed miRNAs were determined by deep sequencing, and compared to unsupplemented/unstimulated controls. Data gained clearly show that PUFAs in fact modulate miRNA expression of both cell types analyzed regardless the presence/absence of an inflammatory stimulator. Moreover, certain miRNAs already linked to vascular inflammation were found to be affected by cellular PUFA enrichment. Hence, vascular inflammation appears to be influenced by dietary fatty acids, inter alia, via PUFA-mediated modulation of the type and amount of miRNAs synthesized by cells involved in the inflammatory process.
format Online
Article
Text
id pubmed-5343820
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-53438202017-03-16 Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study Roessler, Claudia Kuhlmann, Kevin Hellwing, Christine Leimert, Anja Schumann, Julia Int J Mol Sci Article Alteration of miRNAs and dietary polyunsaturated fatty acids (PUFAs) underlies vascular inflammation. PUFAs are known to be incorporated into the cell membrane of monocytes/macrophages or endothelial cells, the major cellular players of vascular diseases, thereby affecting cellular signal transduction. Nevertheless, there are no investigations concerning the PUFA impact on miRNA expression by these cells. With regard to the key role miRNAs play for overall cellular functionality, this study aims to elucidate whether PUFAs affect miRNA expression profiles. To this end, the monocyte/macrophage cell line RAW264.7 and the endothelial cell line TIME were enriched with either docosahexaenoic acid (DHA; n3-PUFA) or arachidonic acid (AA; n6-PUFA) until reaching a stable incorporation into the plasma membrane and, at least in part, exposed to an inflammatory milieu. Expressed miRNAs were determined by deep sequencing, and compared to unsupplemented/unstimulated controls. Data gained clearly show that PUFAs in fact modulate miRNA expression of both cell types analyzed regardless the presence/absence of an inflammatory stimulator. Moreover, certain miRNAs already linked to vascular inflammation were found to be affected by cellular PUFA enrichment. Hence, vascular inflammation appears to be influenced by dietary fatty acids, inter alia, via PUFA-mediated modulation of the type and amount of miRNAs synthesized by cells involved in the inflammatory process. MDPI 2017-01-28 /pmc/articles/PMC5343820/ /pubmed/28134837 http://dx.doi.org/10.3390/ijms18020284 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roessler, Claudia
Kuhlmann, Kevin
Hellwing, Christine
Leimert, Anja
Schumann, Julia
Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title_full Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title_fullStr Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title_full_unstemmed Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title_short Impact of Polyunsaturated Fatty Acids on miRNA Profiles of Monocytes/Macrophages and Endothelial Cells—A Pilot Study
title_sort impact of polyunsaturated fatty acids on mirna profiles of monocytes/macrophages and endothelial cells—a pilot study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343820/
https://www.ncbi.nlm.nih.gov/pubmed/28134837
http://dx.doi.org/10.3390/ijms18020284
work_keys_str_mv AT roesslerclaudia impactofpolyunsaturatedfattyacidsonmirnaprofilesofmonocytesmacrophagesandendothelialcellsapilotstudy
AT kuhlmannkevin impactofpolyunsaturatedfattyacidsonmirnaprofilesofmonocytesmacrophagesandendothelialcellsapilotstudy
AT hellwingchristine impactofpolyunsaturatedfattyacidsonmirnaprofilesofmonocytesmacrophagesandendothelialcellsapilotstudy
AT leimertanja impactofpolyunsaturatedfattyacidsonmirnaprofilesofmonocytesmacrophagesandendothelialcellsapilotstudy
AT schumannjulia impactofpolyunsaturatedfattyacidsonmirnaprofilesofmonocytesmacrophagesandendothelialcellsapilotstudy