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Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma

Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia–reperfusion (IR) injury, this study performed a proteomi...

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Autores principales: Chen, Hsin-Yi, Chou, Hsiu-Chuan, Chang, Shing-Jyh, Liao, En-Chi, Tsai, Yi-Ting, Wei, Yu-Shan, Li, Ji-Min, Lin, Li-Hsun, Lin, Meng-Wei, Chen, Ying-Jen, Chen, Yu-Sheng, Lin, Chih-Chun, Wang, Yi-Shiuan, Ko, Mei-Lan, Chan, Hong-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343869/
https://www.ncbi.nlm.nih.gov/pubmed/28165428
http://dx.doi.org/10.3390/ijms18020334
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author Chen, Hsin-Yi
Chou, Hsiu-Chuan
Chang, Shing-Jyh
Liao, En-Chi
Tsai, Yi-Ting
Wei, Yu-Shan
Li, Ji-Min
Lin, Li-Hsun
Lin, Meng-Wei
Chen, Ying-Jen
Chen, Yu-Sheng
Lin, Chih-Chun
Wang, Yi-Shiuan
Ko, Mei-Lan
Chan, Hong-Lin
author_facet Chen, Hsin-Yi
Chou, Hsiu-Chuan
Chang, Shing-Jyh
Liao, En-Chi
Tsai, Yi-Ting
Wei, Yu-Shan
Li, Ji-Min
Lin, Li-Hsun
Lin, Meng-Wei
Chen, Ying-Jen
Chen, Yu-Sheng
Lin, Chih-Chun
Wang, Yi-Shiuan
Ko, Mei-Lan
Chan, Hong-Lin
author_sort Chen, Hsin-Yi
collection PubMed
description Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia–reperfusion (IR) injury, this study performed a proteomic analysis to qualitatively investigate such alterations resulting from acute glaucoma. The IR injury model combined with the proteomic analysis approach of two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to monitor the protein expression alterations in two groups of specimens (an IR injury group and a control group). The analysis results revealed 221 unique differentially expressed proteins of a total of 1481 proteins in the cornea between the two groups. In addition, 97 of 1206 conjunctival proteins, 90 of 1354 uveal proteins, 61 of 1180 scleral proteins, and 37 of 1204 retinal proteins were differentially expressed. These findings imply that different ocular tissues have different tolerances against IR injury. To sum up, this study utilized the acute glaucoma model combined with 2D-DIGE and MALDI-TOF MS to investigate the IR injury affected protein expression on various ocular tissues, and based on the ratio of protein expression alterations, the alterations in the ocular tissues were in the following order: the cornea, conjunctiva, uvea, sclera, and retina.
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spelling pubmed-53438692017-03-16 Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma Chen, Hsin-Yi Chou, Hsiu-Chuan Chang, Shing-Jyh Liao, En-Chi Tsai, Yi-Ting Wei, Yu-Shan Li, Ji-Min Lin, Li-Hsun Lin, Meng-Wei Chen, Ying-Jen Chen, Yu-Sheng Lin, Chih-Chun Wang, Yi-Shiuan Ko, Mei-Lan Chan, Hong-Lin Int J Mol Sci Article Glaucoma is a group of eye diseases that can cause vision loss and optical nerve damage. To investigate the protein expression alterations in various intraocular tissues (i.e., the cornea, conjunctiva, uvea, retina, and sclera) during ischemia–reperfusion (IR) injury, this study performed a proteomic analysis to qualitatively investigate such alterations resulting from acute glaucoma. The IR injury model combined with the proteomic analysis approach of two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to monitor the protein expression alterations in two groups of specimens (an IR injury group and a control group). The analysis results revealed 221 unique differentially expressed proteins of a total of 1481 proteins in the cornea between the two groups. In addition, 97 of 1206 conjunctival proteins, 90 of 1354 uveal proteins, 61 of 1180 scleral proteins, and 37 of 1204 retinal proteins were differentially expressed. These findings imply that different ocular tissues have different tolerances against IR injury. To sum up, this study utilized the acute glaucoma model combined with 2D-DIGE and MALDI-TOF MS to investigate the IR injury affected protein expression on various ocular tissues, and based on the ratio of protein expression alterations, the alterations in the ocular tissues were in the following order: the cornea, conjunctiva, uvea, sclera, and retina. MDPI 2017-02-05 /pmc/articles/PMC5343869/ /pubmed/28165428 http://dx.doi.org/10.3390/ijms18020334 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Hsin-Yi
Chou, Hsiu-Chuan
Chang, Shing-Jyh
Liao, En-Chi
Tsai, Yi-Ting
Wei, Yu-Shan
Li, Ji-Min
Lin, Li-Hsun
Lin, Meng-Wei
Chen, Ying-Jen
Chen, Yu-Sheng
Lin, Chih-Chun
Wang, Yi-Shiuan
Ko, Mei-Lan
Chan, Hong-Lin
Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title_full Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title_fullStr Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title_full_unstemmed Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title_short Proteomic Analysis of Various Rat Ocular Tissues after Ischemia–Reperfusion Injury and Possible Relevance to Acute Glaucoma
title_sort proteomic analysis of various rat ocular tissues after ischemia–reperfusion injury and possible relevance to acute glaucoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343869/
https://www.ncbi.nlm.nih.gov/pubmed/28165428
http://dx.doi.org/10.3390/ijms18020334
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