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Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect...

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Autores principales: Mok, Pooi Ling, Leow, Sue Ngein, Koh, Avin Ee-Hwan, Mohd Nizam, Hairul Harun, Ding, Suet Lee Shirley, Luu, Chi, Ruhaslizan, Raduan, Wong, Hon Seng, Halim, Wan Haslina Wan Abdul, Ng, Min Hwei, Idrus, Ruszymah Binti Hj., Chowdhury, Shiplu Roy, Bastion, Catherine Mae-Lynn, Subbiah, Suresh Kumar, Higuchi, Akon, Alarfaj, Abdullah A., Then, Kong Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343880/
https://www.ncbi.nlm.nih.gov/pubmed/28208719
http://dx.doi.org/10.3390/ijms18020345
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author Mok, Pooi Ling
Leow, Sue Ngein
Koh, Avin Ee-Hwan
Mohd Nizam, Hairul Harun
Ding, Suet Lee Shirley
Luu, Chi
Ruhaslizan, Raduan
Wong, Hon Seng
Halim, Wan Haslina Wan Abdul
Ng, Min Hwei
Idrus, Ruszymah Binti Hj.
Chowdhury, Shiplu Roy
Bastion, Catherine Mae-Lynn
Subbiah, Suresh Kumar
Higuchi, Akon
Alarfaj, Abdullah A.
Then, Kong Yong
author_facet Mok, Pooi Ling
Leow, Sue Ngein
Koh, Avin Ee-Hwan
Mohd Nizam, Hairul Harun
Ding, Suet Lee Shirley
Luu, Chi
Ruhaslizan, Raduan
Wong, Hon Seng
Halim, Wan Haslina Wan Abdul
Ng, Min Hwei
Idrus, Ruszymah Binti Hj.
Chowdhury, Shiplu Roy
Bastion, Catherine Mae-Lynn
Subbiah, Suresh Kumar
Higuchi, Akon
Alarfaj, Abdullah A.
Then, Kong Yong
author_sort Mok, Pooi Ling
collection PubMed
description Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.
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spelling pubmed-53438802017-03-16 Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer Mok, Pooi Ling Leow, Sue Ngein Koh, Avin Ee-Hwan Mohd Nizam, Hairul Harun Ding, Suet Lee Shirley Luu, Chi Ruhaslizan, Raduan Wong, Hon Seng Halim, Wan Haslina Wan Abdul Ng, Min Hwei Idrus, Ruszymah Binti Hj. Chowdhury, Shiplu Roy Bastion, Catherine Mae-Lynn Subbiah, Suresh Kumar Higuchi, Akon Alarfaj, Abdullah A. Then, Kong Yong Int J Mol Sci Article Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases. MDPI 2017-02-08 /pmc/articles/PMC5343880/ /pubmed/28208719 http://dx.doi.org/10.3390/ijms18020345 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mok, Pooi Ling
Leow, Sue Ngein
Koh, Avin Ee-Hwan
Mohd Nizam, Hairul Harun
Ding, Suet Lee Shirley
Luu, Chi
Ruhaslizan, Raduan
Wong, Hon Seng
Halim, Wan Haslina Wan Abdul
Ng, Min Hwei
Idrus, Ruszymah Binti Hj.
Chowdhury, Shiplu Roy
Bastion, Catherine Mae-Lynn
Subbiah, Suresh Kumar
Higuchi, Akon
Alarfaj, Abdullah A.
Then, Kong Yong
Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title_full Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title_fullStr Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title_full_unstemmed Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title_short Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer
title_sort micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343880/
https://www.ncbi.nlm.nih.gov/pubmed/28208719
http://dx.doi.org/10.3390/ijms18020345
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