Cargando…
Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343887/ https://www.ncbi.nlm.nih.gov/pubmed/28208699 http://dx.doi.org/10.3390/ijms18020352 |
_version_ | 1782513446799015936 |
---|---|
author | Hara, Takato Kojima, Takayuki Matsuzaki, Hiroka Nakamura, Takehiro Yoshida, Eiko Fujiwara, Yasuyuki Yamamoto, Chika Saito, Shinichi Kaji, Toshiyuki |
author_facet | Hara, Takato Kojima, Takayuki Matsuzaki, Hiroka Nakamura, Takehiro Yoshida, Eiko Fujiwara, Yasuyuki Yamamoto, Chika Saito, Shinichi Kaji, Toshiyuki |
author_sort | Hara, Takato |
collection | PubMed |
description | Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is regulated by several cytokines/growth factors, there may be alternative pathways for proteoglycan synthesis aside from downstream pathways activated by these cytokines/growth factors. Here, we investigated organic–inorganic hybrid molecules to determine a variant capable of analyzing the expression of syndecan-4, a transmembrane heparan-sulfate proteoglycan, and identified 1,10-phenanthroline (o-Phen) with or without zinc (Zn-Phen) or rhodium (Rh-Phen). Bovine aortic endothelial cells in culture were treated with these compounds, and the expression of syndecan-4 mRNA and core proteins was determined by real-time reverse transcription polymerase chain reaction and Western blot analysis, respectively. Our findings indicated that o-Phen and Zn-Phen specifically and strongly induced syndecan-4 expression in cultured vascular endothelial cells through activation of the hypoxia-inducible factor-1α/β pathway via inhibition of prolyl hydroxylase-domain-containing protein 2. These results demonstrated an alternative pathway involved in mediating induction of endothelial syndecan-4 expression and revealed organic–inorganic hybrid molecules as effective tools for analyzing biological systems. |
format | Online Article Text |
id | pubmed-5343887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53438872017-03-16 Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells Hara, Takato Kojima, Takayuki Matsuzaki, Hiroka Nakamura, Takehiro Yoshida, Eiko Fujiwara, Yasuyuki Yamamoto, Chika Saito, Shinichi Kaji, Toshiyuki Int J Mol Sci Article Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is regulated by several cytokines/growth factors, there may be alternative pathways for proteoglycan synthesis aside from downstream pathways activated by these cytokines/growth factors. Here, we investigated organic–inorganic hybrid molecules to determine a variant capable of analyzing the expression of syndecan-4, a transmembrane heparan-sulfate proteoglycan, and identified 1,10-phenanthroline (o-Phen) with or without zinc (Zn-Phen) or rhodium (Rh-Phen). Bovine aortic endothelial cells in culture were treated with these compounds, and the expression of syndecan-4 mRNA and core proteins was determined by real-time reverse transcription polymerase chain reaction and Western blot analysis, respectively. Our findings indicated that o-Phen and Zn-Phen specifically and strongly induced syndecan-4 expression in cultured vascular endothelial cells through activation of the hypoxia-inducible factor-1α/β pathway via inhibition of prolyl hydroxylase-domain-containing protein 2. These results demonstrated an alternative pathway involved in mediating induction of endothelial syndecan-4 expression and revealed organic–inorganic hybrid molecules as effective tools for analyzing biological systems. MDPI 2017-02-08 /pmc/articles/PMC5343887/ /pubmed/28208699 http://dx.doi.org/10.3390/ijms18020352 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hara, Takato Kojima, Takayuki Matsuzaki, Hiroka Nakamura, Takehiro Yoshida, Eiko Fujiwara, Yasuyuki Yamamoto, Chika Saito, Shinichi Kaji, Toshiyuki Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title | Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title_full | Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title_fullStr | Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title_full_unstemmed | Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title_short | Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells |
title_sort | induction of syndecan-4 by organic–inorganic hybrid molecules with a 1,10-phenanthroline structure in cultured vascular endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343887/ https://www.ncbi.nlm.nih.gov/pubmed/28208699 http://dx.doi.org/10.3390/ijms18020352 |
work_keys_str_mv | AT haratakato inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT kojimatakayuki inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT matsuzakihiroka inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT nakamuratakehiro inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT yoshidaeiko inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT fujiwarayasuyuki inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT yamamotochika inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT saitoshinichi inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells AT kajitoshiyuki inductionofsyndecan4byorganicinorganichybridmoleculeswitha110phenanthrolinestructureinculturedvascularendothelialcells |