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Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells

Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is...

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Autores principales: Hara, Takato, Kojima, Takayuki, Matsuzaki, Hiroka, Nakamura, Takehiro, Yoshida, Eiko, Fujiwara, Yasuyuki, Yamamoto, Chika, Saito, Shinichi, Kaji, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343887/
https://www.ncbi.nlm.nih.gov/pubmed/28208699
http://dx.doi.org/10.3390/ijms18020352
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author Hara, Takato
Kojima, Takayuki
Matsuzaki, Hiroka
Nakamura, Takehiro
Yoshida, Eiko
Fujiwara, Yasuyuki
Yamamoto, Chika
Saito, Shinichi
Kaji, Toshiyuki
author_facet Hara, Takato
Kojima, Takayuki
Matsuzaki, Hiroka
Nakamura, Takehiro
Yoshida, Eiko
Fujiwara, Yasuyuki
Yamamoto, Chika
Saito, Shinichi
Kaji, Toshiyuki
author_sort Hara, Takato
collection PubMed
description Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is regulated by several cytokines/growth factors, there may be alternative pathways for proteoglycan synthesis aside from downstream pathways activated by these cytokines/growth factors. Here, we investigated organic–inorganic hybrid molecules to determine a variant capable of analyzing the expression of syndecan-4, a transmembrane heparan-sulfate proteoglycan, and identified 1,10-phenanthroline (o-Phen) with or without zinc (Zn-Phen) or rhodium (Rh-Phen). Bovine aortic endothelial cells in culture were treated with these compounds, and the expression of syndecan-4 mRNA and core proteins was determined by real-time reverse transcription polymerase chain reaction and Western blot analysis, respectively. Our findings indicated that o-Phen and Zn-Phen specifically and strongly induced syndecan-4 expression in cultured vascular endothelial cells through activation of the hypoxia-inducible factor-1α/β pathway via inhibition of prolyl hydroxylase-domain-containing protein 2. These results demonstrated an alternative pathway involved in mediating induction of endothelial syndecan-4 expression and revealed organic–inorganic hybrid molecules as effective tools for analyzing biological systems.
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spelling pubmed-53438872017-03-16 Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells Hara, Takato Kojima, Takayuki Matsuzaki, Hiroka Nakamura, Takehiro Yoshida, Eiko Fujiwara, Yasuyuki Yamamoto, Chika Saito, Shinichi Kaji, Toshiyuki Int J Mol Sci Article Organic–inorganic hybrid molecules constitute analytical tools used in biological systems. Vascular endothelial cells synthesize and secrete proteoglycans, which are macromolecules consisting of a core protein and glycosaminoglycan side chains. Although the expression of endothelial proteoglycans is regulated by several cytokines/growth factors, there may be alternative pathways for proteoglycan synthesis aside from downstream pathways activated by these cytokines/growth factors. Here, we investigated organic–inorganic hybrid molecules to determine a variant capable of analyzing the expression of syndecan-4, a transmembrane heparan-sulfate proteoglycan, and identified 1,10-phenanthroline (o-Phen) with or without zinc (Zn-Phen) or rhodium (Rh-Phen). Bovine aortic endothelial cells in culture were treated with these compounds, and the expression of syndecan-4 mRNA and core proteins was determined by real-time reverse transcription polymerase chain reaction and Western blot analysis, respectively. Our findings indicated that o-Phen and Zn-Phen specifically and strongly induced syndecan-4 expression in cultured vascular endothelial cells through activation of the hypoxia-inducible factor-1α/β pathway via inhibition of prolyl hydroxylase-domain-containing protein 2. These results demonstrated an alternative pathway involved in mediating induction of endothelial syndecan-4 expression and revealed organic–inorganic hybrid molecules as effective tools for analyzing biological systems. MDPI 2017-02-08 /pmc/articles/PMC5343887/ /pubmed/28208699 http://dx.doi.org/10.3390/ijms18020352 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hara, Takato
Kojima, Takayuki
Matsuzaki, Hiroka
Nakamura, Takehiro
Yoshida, Eiko
Fujiwara, Yasuyuki
Yamamoto, Chika
Saito, Shinichi
Kaji, Toshiyuki
Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title_full Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title_fullStr Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title_full_unstemmed Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title_short Induction of Syndecan-4 by Organic–Inorganic Hybrid Molecules with a 1,10-Phenanthroline Structure in Cultured Vascular Endothelial Cells
title_sort induction of syndecan-4 by organic–inorganic hybrid molecules with a 1,10-phenanthroline structure in cultured vascular endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343887/
https://www.ncbi.nlm.nih.gov/pubmed/28208699
http://dx.doi.org/10.3390/ijms18020352
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