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Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering

As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an...

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Autores principales: Spagnolo, Alessandro, Mondello, Vincenzo, Larignon, Philippe, Villaume, Sandra, Rabenoelina, Fanja, Clément, Christophe, Fontaine, Florence
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343928/
https://www.ncbi.nlm.nih.gov/pubmed/28208805
http://dx.doi.org/10.3390/ijms18020393
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author Spagnolo, Alessandro
Mondello, Vincenzo
Larignon, Philippe
Villaume, Sandra
Rabenoelina, Fanja
Clément, Christophe
Fontaine, Florence
author_facet Spagnolo, Alessandro
Mondello, Vincenzo
Larignon, Philippe
Villaume, Sandra
Rabenoelina, Fanja
Clément, Christophe
Fontaine, Florence
author_sort Spagnolo, Alessandro
collection PubMed
description As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents, Neofusicoccum parvum and Diplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase.
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spelling pubmed-53439282017-03-16 Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering Spagnolo, Alessandro Mondello, Vincenzo Larignon, Philippe Villaume, Sandra Rabenoelina, Fanja Clément, Christophe Fontaine, Florence Int J Mol Sci Article As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents, Neofusicoccum parvum and Diplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase. MDPI 2017-02-13 /pmc/articles/PMC5343928/ /pubmed/28208805 http://dx.doi.org/10.3390/ijms18020393 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spagnolo, Alessandro
Mondello, Vincenzo
Larignon, Philippe
Villaume, Sandra
Rabenoelina, Fanja
Clément, Christophe
Fontaine, Florence
Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title_full Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title_fullStr Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title_full_unstemmed Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title_short Defense Responses in Grapevine (cv. Mourvèdre) after Inoculation with the Botryosphaeria Dieback Pathogens Neofusicoccum parvum and Diplodia seriata and Their Relationship with Flowering
title_sort defense responses in grapevine (cv. mourvèdre) after inoculation with the botryosphaeria dieback pathogens neofusicoccum parvum and diplodia seriata and their relationship with flowering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343928/
https://www.ncbi.nlm.nih.gov/pubmed/28208805
http://dx.doi.org/10.3390/ijms18020393
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