Cargando…

An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach

Sexually transmitted infections (STIs) are caused by a wide variety of bacteria, viruses, and parasites that are transmitted from one person to another primarily by vaginal, anal, or oral sexual contact. Syphilis is a serious disease caused by a sexually transmitted infection. Syphilis is caused by...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar Jaiswal, Arun, Tiwari, Sandeep, Jamal, Syed Babar, Barh, Debmalya, Azevedo, Vasco, Soares, Siomar C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343936/
https://www.ncbi.nlm.nih.gov/pubmed/28216574
http://dx.doi.org/10.3390/ijms18020402
_version_ 1782513458089033728
author Kumar Jaiswal, Arun
Tiwari, Sandeep
Jamal, Syed Babar
Barh, Debmalya
Azevedo, Vasco
Soares, Siomar C.
author_facet Kumar Jaiswal, Arun
Tiwari, Sandeep
Jamal, Syed Babar
Barh, Debmalya
Azevedo, Vasco
Soares, Siomar C.
author_sort Kumar Jaiswal, Arun
collection PubMed
description Sexually transmitted infections (STIs) are caused by a wide variety of bacteria, viruses, and parasites that are transmitted from one person to another primarily by vaginal, anal, or oral sexual contact. Syphilis is a serious disease caused by a sexually transmitted infection. Syphilis is caused by the bacterium Treponema pallidum subspecies pallidum. Treponema pallidum (T. pallidum) is a motile, gram-negative spirochete, which can be transmitted both sexually and from mother to child, and can invade virtually any organ or structure in the human body. The current worldwide prevalence of syphilis emphasizes the need for continued preventive measures and strategies. Unfortunately, effective measures are limited. In this study, we focus on the identification of vaccine targets and putative drugs against syphilis disease using reverse vaccinology and subtractive genomics. We compared 13 strains of T. pallidum using T. pallidum Nichols as the reference genome. Using an in silicoapproach, four pathogenic islands were detected in the genome of T. pallidum Nichols. We identified 15 putative antigenic proteins and sixdrug targets through reverse vaccinology and subtractive genomics, respectively, which can be used as candidate therapeutic targets in the future.
format Online
Article
Text
id pubmed-5343936
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-53439362017-03-16 An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach Kumar Jaiswal, Arun Tiwari, Sandeep Jamal, Syed Babar Barh, Debmalya Azevedo, Vasco Soares, Siomar C. Int J Mol Sci Article Sexually transmitted infections (STIs) are caused by a wide variety of bacteria, viruses, and parasites that are transmitted from one person to another primarily by vaginal, anal, or oral sexual contact. Syphilis is a serious disease caused by a sexually transmitted infection. Syphilis is caused by the bacterium Treponema pallidum subspecies pallidum. Treponema pallidum (T. pallidum) is a motile, gram-negative spirochete, which can be transmitted both sexually and from mother to child, and can invade virtually any organ or structure in the human body. The current worldwide prevalence of syphilis emphasizes the need for continued preventive measures and strategies. Unfortunately, effective measures are limited. In this study, we focus on the identification of vaccine targets and putative drugs against syphilis disease using reverse vaccinology and subtractive genomics. We compared 13 strains of T. pallidum using T. pallidum Nichols as the reference genome. Using an in silicoapproach, four pathogenic islands were detected in the genome of T. pallidum Nichols. We identified 15 putative antigenic proteins and sixdrug targets through reverse vaccinology and subtractive genomics, respectively, which can be used as candidate therapeutic targets in the future. MDPI 2017-02-14 /pmc/articles/PMC5343936/ /pubmed/28216574 http://dx.doi.org/10.3390/ijms18020402 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumar Jaiswal, Arun
Tiwari, Sandeep
Jamal, Syed Babar
Barh, Debmalya
Azevedo, Vasco
Soares, Siomar C.
An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title_full An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title_fullStr An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title_full_unstemmed An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title_short An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach
title_sort in silico identification of common putative vaccine candidates against treponema pallidum: a reverse vaccinology and subtractive genomics based approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343936/
https://www.ncbi.nlm.nih.gov/pubmed/28216574
http://dx.doi.org/10.3390/ijms18020402
work_keys_str_mv AT kumarjaiswalarun aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT tiwarisandeep aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT jamalsyedbabar aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT barhdebmalya aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT azevedovasco aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT soaressiomarc aninsilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT kumarjaiswalarun insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT tiwarisandeep insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT jamalsyedbabar insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT barhdebmalya insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT azevedovasco insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach
AT soaressiomarc insilicoidentificationofcommonputativevaccinecandidatesagainsttreponemapallidumareversevaccinologyandsubtractivegenomicsbasedapproach