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A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on sever...

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Autores principales: Theodoro-Júnior, Osmar Aparecido, Righetti, Renato Fraga, Almeida-Reis, Rafael, Martins-Oliveira, Bruno Tadeu, Oliva, Leandro Vilela, Prado, Carla Máximo, Saraiva-Romanholo, Beatriz Mangueira, Leick, Edna Aparecida, Pinheiro, Nathalia Montouro, Lobo, Yara Aparecida, Martins, Mílton de Arruda, Oliva, Maria Luiza Vilela, Tibério, Iolanda de Fátima Lopes Calvo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343937/
https://www.ncbi.nlm.nih.gov/pubmed/28216579
http://dx.doi.org/10.3390/ijms18020403
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author Theodoro-Júnior, Osmar Aparecido
Righetti, Renato Fraga
Almeida-Reis, Rafael
Martins-Oliveira, Bruno Tadeu
Oliva, Leandro Vilela
Prado, Carla Máximo
Saraiva-Romanholo, Beatriz Mangueira
Leick, Edna Aparecida
Pinheiro, Nathalia Montouro
Lobo, Yara Aparecida
Martins, Mílton de Arruda
Oliva, Maria Luiza Vilela
Tibério, Iolanda de Fátima Lopes Calvo
author_facet Theodoro-Júnior, Osmar Aparecido
Righetti, Renato Fraga
Almeida-Reis, Rafael
Martins-Oliveira, Bruno Tadeu
Oliva, Leandro Vilela
Prado, Carla Máximo
Saraiva-Romanholo, Beatriz Mangueira
Leick, Edna Aparecida
Pinheiro, Nathalia Montouro
Lobo, Yara Aparecida
Martins, Mílton de Arruda
Oliva, Maria Luiza Vilela
Tibério, Iolanda de Fátima Lopes Calvo
author_sort Theodoro-Júnior, Osmar Aparecido
collection PubMed
description Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.
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spelling pubmed-53439372017-03-16 A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice Theodoro-Júnior, Osmar Aparecido Righetti, Renato Fraga Almeida-Reis, Rafael Martins-Oliveira, Bruno Tadeu Oliva, Leandro Vilela Prado, Carla Máximo Saraiva-Romanholo, Beatriz Mangueira Leick, Edna Aparecida Pinheiro, Nathalia Montouro Lobo, Yara Aparecida Martins, Mílton de Arruda Oliva, Maria Luiza Vilela Tibério, Iolanda de Fátima Lopes Calvo Int J Mol Sci Article Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management. MDPI 2017-02-14 /pmc/articles/PMC5343937/ /pubmed/28216579 http://dx.doi.org/10.3390/ijms18020403 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Theodoro-Júnior, Osmar Aparecido
Righetti, Renato Fraga
Almeida-Reis, Rafael
Martins-Oliveira, Bruno Tadeu
Oliva, Leandro Vilela
Prado, Carla Máximo
Saraiva-Romanholo, Beatriz Mangueira
Leick, Edna Aparecida
Pinheiro, Nathalia Montouro
Lobo, Yara Aparecida
Martins, Mílton de Arruda
Oliva, Maria Luiza Vilela
Tibério, Iolanda de Fátima Lopes Calvo
A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title_full A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title_fullStr A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title_full_unstemmed A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title_short A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
title_sort plant proteinase inhibitor from enterolobium contortisiliquum attenuates pulmonary mechanics, inflammation and remodeling induced by elastase in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343937/
https://www.ncbi.nlm.nih.gov/pubmed/28216579
http://dx.doi.org/10.3390/ijms18020403
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