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A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice
Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on sever...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343937/ https://www.ncbi.nlm.nih.gov/pubmed/28216579 http://dx.doi.org/10.3390/ijms18020403 |
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author | Theodoro-Júnior, Osmar Aparecido Righetti, Renato Fraga Almeida-Reis, Rafael Martins-Oliveira, Bruno Tadeu Oliva, Leandro Vilela Prado, Carla Máximo Saraiva-Romanholo, Beatriz Mangueira Leick, Edna Aparecida Pinheiro, Nathalia Montouro Lobo, Yara Aparecida Martins, Mílton de Arruda Oliva, Maria Luiza Vilela Tibério, Iolanda de Fátima Lopes Calvo |
author_facet | Theodoro-Júnior, Osmar Aparecido Righetti, Renato Fraga Almeida-Reis, Rafael Martins-Oliveira, Bruno Tadeu Oliva, Leandro Vilela Prado, Carla Máximo Saraiva-Romanholo, Beatriz Mangueira Leick, Edna Aparecida Pinheiro, Nathalia Montouro Lobo, Yara Aparecida Martins, Mílton de Arruda Oliva, Maria Luiza Vilela Tibério, Iolanda de Fátima Lopes Calvo |
author_sort | Theodoro-Júnior, Osmar Aparecido |
collection | PubMed |
description | Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management. |
format | Online Article Text |
id | pubmed-5343937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-53439372017-03-16 A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice Theodoro-Júnior, Osmar Aparecido Righetti, Renato Fraga Almeida-Reis, Rafael Martins-Oliveira, Bruno Tadeu Oliva, Leandro Vilela Prado, Carla Máximo Saraiva-Romanholo, Beatriz Mangueira Leick, Edna Aparecida Pinheiro, Nathalia Montouro Lobo, Yara Aparecida Martins, Mílton de Arruda Oliva, Maria Luiza Vilela Tibério, Iolanda de Fátima Lopes Calvo Int J Mol Sci Article Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management. MDPI 2017-02-14 /pmc/articles/PMC5343937/ /pubmed/28216579 http://dx.doi.org/10.3390/ijms18020403 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Theodoro-Júnior, Osmar Aparecido Righetti, Renato Fraga Almeida-Reis, Rafael Martins-Oliveira, Bruno Tadeu Oliva, Leandro Vilela Prado, Carla Máximo Saraiva-Romanholo, Beatriz Mangueira Leick, Edna Aparecida Pinheiro, Nathalia Montouro Lobo, Yara Aparecida Martins, Mílton de Arruda Oliva, Maria Luiza Vilela Tibério, Iolanda de Fátima Lopes Calvo A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title | A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title_full | A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title_fullStr | A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title_full_unstemmed | A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title_short | A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice |
title_sort | plant proteinase inhibitor from enterolobium contortisiliquum attenuates pulmonary mechanics, inflammation and remodeling induced by elastase in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343937/ https://www.ncbi.nlm.nih.gov/pubmed/28216579 http://dx.doi.org/10.3390/ijms18020403 |
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