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Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains

Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of c...

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Autores principales: Fusco, Alessandra, Coretti, Lorena, Savio, Vittoria, Buommino, Elisabetta, Lembo, Francesca, Donnarumma, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343948/
https://www.ncbi.nlm.nih.gov/pubmed/28212280
http://dx.doi.org/10.3390/ijms18020414
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author Fusco, Alessandra
Coretti, Lorena
Savio, Vittoria
Buommino, Elisabetta
Lembo, Francesca
Donnarumma, Giovanna
author_facet Fusco, Alessandra
Coretti, Lorena
Savio, Vittoria
Buommino, Elisabetta
Lembo, Francesca
Donnarumma, Giovanna
author_sort Fusco, Alessandra
collection PubMed
description Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms on the abiotic surface of the urinary catheters. Since, to date, the role of P. mirabilis in the etiopathogenesis of different types of urinary tract infections is not well established, in this study we sought to characterize two different clinically isolated strains of P. mirabilis (PM1 and PM2) with distinctive phenotypes and analyzed various virulence factors possibly implicated in the ability to induce UTIs and CAUTIs. In particular, we analyzed motility, biofilm formation both on abiotic and biotic surfaces of PM1 and PM2 and paralleled these parameters with the ability to induce an inflammatory response in an epithelial cell model. Results showed that PM1 displayed major motility and a capacity to form biofilm and was associated with an anti-inflammatory response of host cells. Conversely, PM2 exhibited lack motility and a had slower organization in biofilm but promoted an increase of proinflammatory cytokine expression in infected epithelial cells. Our study provides data useful to start uncovering the pathologic basis of P. mirabilis-associated urinary infections. The evidence of different virulence factors expressed by PM1 and PM2 highlights the possibility to use precise and personalized therapies targeting specific virulence pathways.
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spelling pubmed-53439482017-03-16 Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains Fusco, Alessandra Coretti, Lorena Savio, Vittoria Buommino, Elisabetta Lembo, Francesca Donnarumma, Giovanna Int J Mol Sci Article Urinary tract infections (UTIs) and catheter-associated UTIs (CAUTIs) are the principal hospital-acquired infections. Proteus mirabilis is characterized by several virulence factors able to promote adhesion and biofilm formation and ameliorate the colonization of urinary tract and the formation of crystalline biofilms on the abiotic surface of the urinary catheters. Since, to date, the role of P. mirabilis in the etiopathogenesis of different types of urinary tract infections is not well established, in this study we sought to characterize two different clinically isolated strains of P. mirabilis (PM1 and PM2) with distinctive phenotypes and analyzed various virulence factors possibly implicated in the ability to induce UTIs and CAUTIs. In particular, we analyzed motility, biofilm formation both on abiotic and biotic surfaces of PM1 and PM2 and paralleled these parameters with the ability to induce an inflammatory response in an epithelial cell model. Results showed that PM1 displayed major motility and a capacity to form biofilm and was associated with an anti-inflammatory response of host cells. Conversely, PM2 exhibited lack motility and a had slower organization in biofilm but promoted an increase of proinflammatory cytokine expression in infected epithelial cells. Our study provides data useful to start uncovering the pathologic basis of P. mirabilis-associated urinary infections. The evidence of different virulence factors expressed by PM1 and PM2 highlights the possibility to use precise and personalized therapies targeting specific virulence pathways. MDPI 2017-02-15 /pmc/articles/PMC5343948/ /pubmed/28212280 http://dx.doi.org/10.3390/ijms18020414 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fusco, Alessandra
Coretti, Lorena
Savio, Vittoria
Buommino, Elisabetta
Lembo, Francesca
Donnarumma, Giovanna
Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title_full Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title_fullStr Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title_full_unstemmed Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title_short Biofilm Formation and Immunomodulatory Activity of Proteus mirabilis Clinically Isolated Strains
title_sort biofilm formation and immunomodulatory activity of proteus mirabilis clinically isolated strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343948/
https://www.ncbi.nlm.nih.gov/pubmed/28212280
http://dx.doi.org/10.3390/ijms18020414
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