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Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc

Background: Degenerative diseases are a medical, social, and economic problem worldwide. The most significant factors predisposing the development of degenerative changes in intervertebral discs are a low density and poor biosynthetic potential of the cells. Therefore, stem cell therapy in this case...

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Autores principales: Volkova, Nataliia, Yukhta, Mariia, Goltsev, Anatoliy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344241/
https://www.ncbi.nlm.nih.gov/pubmed/28536410
http://dx.doi.org/10.3390/biomedicines3030237
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author Volkova, Nataliia
Yukhta, Mariia
Goltsev, Anatoliy
author_facet Volkova, Nataliia
Yukhta, Mariia
Goltsev, Anatoliy
author_sort Volkova, Nataliia
collection PubMed
description Background: Degenerative diseases are a medical, social, and economic problem worldwide. The most significant factors predisposing the development of degenerative changes in intervertebral discs are a low density and poor biosynthetic potential of the cells. Therefore, stem cell therapy in this case should show high clinical efficiency. Methods: The research aim was to evaluate the regenerative potential of cryopreserved mesenchymal stem cells (MSCs) upon degenerative changes in intervertebral discs. Rats with simulated degenerative damage of the intervertebral disc Co6–Co7 were administrated with 0.5 × 10(6) of either native or cryopreserved cells on a collagen sponge to the defect area. The results of experiments were histomorphometrically evaluated on the 30th, 60th, and 90th days after treatment. Results: The restoration of tears, clefts, and collagen fiber fragmentations was noted on the 60th and 90th day after administration of native and cryopreserved MSCs respectively. An increase in fibrochondrocyte density got ahead of the annulus fibrosus height recovery. In the control group without treatment the regeneration was hardly observed. Conclusion: The use of MSCs promotes the restoration of the degenerated intervertebral disc. Cryopreserved MSCs have a “lag” therapeutic effect at the early stages, but show similar results to the native analogue on the 90th day after administration.
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spelling pubmed-53442412017-05-23 Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc Volkova, Nataliia Yukhta, Mariia Goltsev, Anatoliy Biomedicines Article Background: Degenerative diseases are a medical, social, and economic problem worldwide. The most significant factors predisposing the development of degenerative changes in intervertebral discs are a low density and poor biosynthetic potential of the cells. Therefore, stem cell therapy in this case should show high clinical efficiency. Methods: The research aim was to evaluate the regenerative potential of cryopreserved mesenchymal stem cells (MSCs) upon degenerative changes in intervertebral discs. Rats with simulated degenerative damage of the intervertebral disc Co6–Co7 were administrated with 0.5 × 10(6) of either native or cryopreserved cells on a collagen sponge to the defect area. The results of experiments were histomorphometrically evaluated on the 30th, 60th, and 90th days after treatment. Results: The restoration of tears, clefts, and collagen fiber fragmentations was noted on the 60th and 90th day after administration of native and cryopreserved MSCs respectively. An increase in fibrochondrocyte density got ahead of the annulus fibrosus height recovery. In the control group without treatment the regeneration was hardly observed. Conclusion: The use of MSCs promotes the restoration of the degenerated intervertebral disc. Cryopreserved MSCs have a “lag” therapeutic effect at the early stages, but show similar results to the native analogue on the 90th day after administration. MDPI 2015-08-07 /pmc/articles/PMC5344241/ /pubmed/28536410 http://dx.doi.org/10.3390/biomedicines3030237 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Volkova, Nataliia
Yukhta, Mariia
Goltsev, Anatoliy
Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title_full Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title_fullStr Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title_full_unstemmed Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title_short Cryopreserved Mesenchymal Stem Cells Stimulate Regeneration in an Intervertebral Disc
title_sort cryopreserved mesenchymal stem cells stimulate regeneration in an intervertebral disc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344241/
https://www.ncbi.nlm.nih.gov/pubmed/28536410
http://dx.doi.org/10.3390/biomedicines3030237
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