Cargando…

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

Rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents Toxoplasma, Echinococcus and Toxocara. Monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. Rodents were trapped a...

Descripción completa

Detalles Bibliográficos
Autores principales: Krücken, Jürgen, Blümke, Julia, Maaz, Denny, Demeler, Janina, Ramünke, Sabrina, Antolová, Daniela, Schaper, Roland, von Samson-Himmelstjerna, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344343/
https://www.ncbi.nlm.nih.gov/pubmed/28278269
http://dx.doi.org/10.1371/journal.pone.0172829
_version_ 1782513521166123008
author Krücken, Jürgen
Blümke, Julia
Maaz, Denny
Demeler, Janina
Ramünke, Sabrina
Antolová, Daniela
Schaper, Roland
von Samson-Himmelstjerna, Georg
author_facet Krücken, Jürgen
Blümke, Julia
Maaz, Denny
Demeler, Janina
Ramünke, Sabrina
Antolová, Daniela
Schaper, Roland
von Samson-Himmelstjerna, Georg
author_sort Krücken, Jürgen
collection PubMed
description Rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents Toxoplasma, Echinococcus and Toxocara. Monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. Rodents were trapped at four sites in Berlin, two near the city center, two at the periphery. PCRs were conducted to detect Coccidia (target ITS-1) and specifically Toxoplasma gondii (repetitive element) in brain and ascarids (ITS-2) in muscle or brain tissue. During necropsies, metacestodes were collected and identified using ITS-2 and 12S rRNA PCRs. An ELISA to detect antibodies against Toxocara canis ES antigens was performed. Within the 257 examined rodents, the most frequently observed parasite was Frenkelia glareoli predominantly found in Myodes glareolus. T. gondii was only detected in 12 rodents and Microtus spp. (although strongly underrepresented) had a significantly increased chance of being positive. Neither Echinococcus nor typical Taenia parasites of dogs and cats were found but Mesocestoides litteratus and Taenia martis metacestodes were identified which can cause severe peritoneal or ocular cysticercosis in dogs, primates and humans. Using PCR, the ascarids T. canis (n = 8), Toxocara cati (4) and Parascaris sp. (1) were detected predominantly in muscles. Seroprevalence of T. canis was 14.2% and ELISA was thus more sensitive than PCR to detect infection with this parasite. Non-parametric multidimensional scaling and cluster analysis revealed that parasite communities could be grouped into an urban and a peri-urban cluster with high frequency of ascarid-positive rodents in urban and high frequency of F. glareoli in peri-urban sites. Prevalence rates of parasites in rodents with potential impact for human or veterinary public health are considerable and the monitoring of transmission cycles of carnivore parasites in intermediate rodent hosts is recommended to estimate the health risks arising from wild and domesticated carnivores.
format Online
Article
Text
id pubmed-5344343
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53443432017-03-29 Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany Krücken, Jürgen Blümke, Julia Maaz, Denny Demeler, Janina Ramünke, Sabrina Antolová, Daniela Schaper, Roland von Samson-Himmelstjerna, Georg PLoS One Research Article Rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents Toxoplasma, Echinococcus and Toxocara. Monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. Rodents were trapped at four sites in Berlin, two near the city center, two at the periphery. PCRs were conducted to detect Coccidia (target ITS-1) and specifically Toxoplasma gondii (repetitive element) in brain and ascarids (ITS-2) in muscle or brain tissue. During necropsies, metacestodes were collected and identified using ITS-2 and 12S rRNA PCRs. An ELISA to detect antibodies against Toxocara canis ES antigens was performed. Within the 257 examined rodents, the most frequently observed parasite was Frenkelia glareoli predominantly found in Myodes glareolus. T. gondii was only detected in 12 rodents and Microtus spp. (although strongly underrepresented) had a significantly increased chance of being positive. Neither Echinococcus nor typical Taenia parasites of dogs and cats were found but Mesocestoides litteratus and Taenia martis metacestodes were identified which can cause severe peritoneal or ocular cysticercosis in dogs, primates and humans. Using PCR, the ascarids T. canis (n = 8), Toxocara cati (4) and Parascaris sp. (1) were detected predominantly in muscles. Seroprevalence of T. canis was 14.2% and ELISA was thus more sensitive than PCR to detect infection with this parasite. Non-parametric multidimensional scaling and cluster analysis revealed that parasite communities could be grouped into an urban and a peri-urban cluster with high frequency of ascarid-positive rodents in urban and high frequency of F. glareoli in peri-urban sites. Prevalence rates of parasites in rodents with potential impact for human or veterinary public health are considerable and the monitoring of transmission cycles of carnivore parasites in intermediate rodent hosts is recommended to estimate the health risks arising from wild and domesticated carnivores. Public Library of Science 2017-03-09 /pmc/articles/PMC5344343/ /pubmed/28278269 http://dx.doi.org/10.1371/journal.pone.0172829 Text en © 2017 Krücken et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Krücken, Jürgen
Blümke, Julia
Maaz, Denny
Demeler, Janina
Ramünke, Sabrina
Antolová, Daniela
Schaper, Roland
von Samson-Himmelstjerna, Georg
Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title_full Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title_fullStr Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title_full_unstemmed Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title_short Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany
title_sort small rodents as paratenic or intermediate hosts of carnivore parasites in berlin, germany
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344343/
https://www.ncbi.nlm.nih.gov/pubmed/28278269
http://dx.doi.org/10.1371/journal.pone.0172829
work_keys_str_mv AT kruckenjurgen smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT blumkejulia smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT maazdenny smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT demelerjanina smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT ramunkesabrina smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT antolovadaniela smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT schaperroland smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany
AT vonsamsonhimmelstjernageorg smallrodentsasparatenicorintermediatehostsofcarnivoreparasitesinberlingermany