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Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship

This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.D...

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Autores principales: Linc, Gabriella, Gaál, Eszter, Molnár, István, Icsó, Diana, Badaeva, Ekaterina, Molnár-Láng, Márta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344461/
https://www.ncbi.nlm.nih.gov/pubmed/28278169
http://dx.doi.org/10.1371/journal.pone.0173623
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author Linc, Gabriella
Gaál, Eszter
Molnár, István
Icsó, Diana
Badaeva, Ekaterina
Molnár-Láng, Márta
author_facet Linc, Gabriella
Gaál, Eszter
Molnár, István
Icsó, Diana
Badaeva, Ekaterina
Molnár-Láng, Márta
author_sort Linc, Gabriella
collection PubMed
description This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes. Of the seven microsatellite markers tested only the (GAA)(n) trinucleotide sequence was appropriate for use as a single chromosome marker for the P. spicata A(S) chromosome. Based on COS marker analysis, the phylogenetic relationship between diploid wheatgrasses and the hexaploid bread wheat genomes was established. These findings confirmed that the J and E genomes are in neighbouring clusters.
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spelling pubmed-53444612017-03-29 Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship Linc, Gabriella Gaál, Eszter Molnár, István Icsó, Diana Badaeva, Ekaterina Molnár-Láng, Márta PLoS One Research Article This paper reports detailed FISH-based karyotypes for three diploid wheatgrass species Agropyron cristatum (L.) Beauv., Thinopyrum bessarabicum (Savul.&Rayss) A. Löve, Pseudoroegneria spicata (Pursh) A. Löve, the supposed ancestors of hexaploid Thinopyrum intermedium (Host) Barkworth & D.R.Dewey, compiled using DNA repeats and comparative genome analysis based on COS markers. Fluorescence in situ hybridization (FISH) with repetitive DNA probes proved suitable for the identification of individual chromosomes in the diploid JJ, StSt and PP genomes. Of the seven microsatellite markers tested only the (GAA)(n) trinucleotide sequence was appropriate for use as a single chromosome marker for the P. spicata A(S) chromosome. Based on COS marker analysis, the phylogenetic relationship between diploid wheatgrasses and the hexaploid bread wheat genomes was established. These findings confirmed that the J and E genomes are in neighbouring clusters. Public Library of Science 2017-03-09 /pmc/articles/PMC5344461/ /pubmed/28278169 http://dx.doi.org/10.1371/journal.pone.0173623 Text en © 2017 Linc et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Linc, Gabriella
Gaál, Eszter
Molnár, István
Icsó, Diana
Badaeva, Ekaterina
Molnár-Láng, Márta
Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title_full Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title_fullStr Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title_full_unstemmed Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title_short Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
title_sort molecular cytogenetic (fish) and genome analysis of diploid wheatgrasses and their phylogenetic relationship
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344461/
https://www.ncbi.nlm.nih.gov/pubmed/28278169
http://dx.doi.org/10.1371/journal.pone.0173623
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