Cargando…

Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children

The gut microbiota represents a complex and diverse ecosystem with a profound impact on human health, promoting immune maturation, and host metabolism as well as colonization resistance. Important members that have often been disregarded are the methanogenic archaea. Methanogenic archaea reduce hydr...

Descripción completa

Detalles Bibliográficos
Autores principales: van de Pol, Jeroen A. A., van Best, Niels, Mbakwa, Catherine A., Thijs, Carel, Savelkoul, Paul H., Arts, Ilja C. W., Hornef, Mathias W., Mommers, Monique, Penders, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344914/
https://www.ncbi.nlm.nih.gov/pubmed/28344572
http://dx.doi.org/10.3389/fmicb.2017.00355
_version_ 1782513611706466304
author van de Pol, Jeroen A. A.
van Best, Niels
Mbakwa, Catherine A.
Thijs, Carel
Savelkoul, Paul H.
Arts, Ilja C. W.
Hornef, Mathias W.
Mommers, Monique
Penders, John
author_facet van de Pol, Jeroen A. A.
van Best, Niels
Mbakwa, Catherine A.
Thijs, Carel
Savelkoul, Paul H.
Arts, Ilja C. W.
Hornef, Mathias W.
Mommers, Monique
Penders, John
author_sort van de Pol, Jeroen A. A.
collection PubMed
description The gut microbiota represents a complex and diverse ecosystem with a profound impact on human health, promoting immune maturation, and host metabolism as well as colonization resistance. Important members that have often been disregarded are the methanogenic archaea. Methanogenic archaea reduce hydrogen levels via the production of methane, thereby stimulating food fermentation by saccharolytic bacteria. On the other hand, colonization by archaea has been suggested to promote a number of gastrointestinal and metabolic diseases such as colorectal cancer, inflammatory bowel disease, and obesity. Archaea have been shown to be absent during infancy while omnipresent in school-aged children, suggesting that colonization may result from environmental exposure during childhood. The factors that determine the acquisition of methanogenic archaea, however, have remained undefined. Therefore, we aimed to explore determinants associated with the acquisition of the two main gastrointestinal archaeal species, Methanobrevibacter smithii and Methanosphaera stadtmanae, in children. Within the context of the KOALA Birth Cohort Study, fecal samples from 472 children aged 6–10 years were analyzed for the abundance of M. smithii and M. stadtmanae using qPCR. Environmental factors such as diet, lifestyle, hygiene, child rearing, and medication were recorded by repeated questionnaires. The relationship between these determinants and the presence and abundance of archaea was analyzed by logistic and linear regression respectively. Three hundred and sixty-nine out of the 472 children (78.2%) were colonized by M. smithii, and 39 out of the 472 children (8.3%) by M. stadtmanae. The consumption of organic yogurt (odds ratio: 4.25, CI95: 1.51; 11.95) and the consumption of organic milk (odds ratio: 5.58, CI95: 1.83; 17.01) were positively associated with the presence of M. smithii. We subsequently screened raw milk, processed milk, and yogurt samples for methanogens. We identified milk products as possible source for M. smithii, but not M. stadtmanae. In conclusion, M. smithii seems present in milk products and their consumption may determine archaeal gut colonization in children. For the first time, a large variety of determinants have been explored in association with gut colonization by methanogenic archaea. Although more information is needed to confirm and unravel the mechanisms in detail, it provides new insights on microbial colonization processes in early life.
format Online
Article
Text
id pubmed-5344914
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-53449142017-03-24 Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children van de Pol, Jeroen A. A. van Best, Niels Mbakwa, Catherine A. Thijs, Carel Savelkoul, Paul H. Arts, Ilja C. W. Hornef, Mathias W. Mommers, Monique Penders, John Front Microbiol Microbiology The gut microbiota represents a complex and diverse ecosystem with a profound impact on human health, promoting immune maturation, and host metabolism as well as colonization resistance. Important members that have often been disregarded are the methanogenic archaea. Methanogenic archaea reduce hydrogen levels via the production of methane, thereby stimulating food fermentation by saccharolytic bacteria. On the other hand, colonization by archaea has been suggested to promote a number of gastrointestinal and metabolic diseases such as colorectal cancer, inflammatory bowel disease, and obesity. Archaea have been shown to be absent during infancy while omnipresent in school-aged children, suggesting that colonization may result from environmental exposure during childhood. The factors that determine the acquisition of methanogenic archaea, however, have remained undefined. Therefore, we aimed to explore determinants associated with the acquisition of the two main gastrointestinal archaeal species, Methanobrevibacter smithii and Methanosphaera stadtmanae, in children. Within the context of the KOALA Birth Cohort Study, fecal samples from 472 children aged 6–10 years were analyzed for the abundance of M. smithii and M. stadtmanae using qPCR. Environmental factors such as diet, lifestyle, hygiene, child rearing, and medication were recorded by repeated questionnaires. The relationship between these determinants and the presence and abundance of archaea was analyzed by logistic and linear regression respectively. Three hundred and sixty-nine out of the 472 children (78.2%) were colonized by M. smithii, and 39 out of the 472 children (8.3%) by M. stadtmanae. The consumption of organic yogurt (odds ratio: 4.25, CI95: 1.51; 11.95) and the consumption of organic milk (odds ratio: 5.58, CI95: 1.83; 17.01) were positively associated with the presence of M. smithii. We subsequently screened raw milk, processed milk, and yogurt samples for methanogens. We identified milk products as possible source for M. smithii, but not M. stadtmanae. In conclusion, M. smithii seems present in milk products and their consumption may determine archaeal gut colonization in children. For the first time, a large variety of determinants have been explored in association with gut colonization by methanogenic archaea. Although more information is needed to confirm and unravel the mechanisms in detail, it provides new insights on microbial colonization processes in early life. Frontiers Media S.A. 2017-03-10 /pmc/articles/PMC5344914/ /pubmed/28344572 http://dx.doi.org/10.3389/fmicb.2017.00355 Text en Copyright © 2017 van de Pol, van Best, Mbakwa, Thijs, Savelkoul, Arts, Hornef, Mommers and Penders. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
van de Pol, Jeroen A. A.
van Best, Niels
Mbakwa, Catherine A.
Thijs, Carel
Savelkoul, Paul H.
Arts, Ilja C. W.
Hornef, Mathias W.
Mommers, Monique
Penders, John
Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title_full Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title_fullStr Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title_full_unstemmed Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title_short Gut Colonization by Methanogenic Archaea Is Associated with Organic Dairy Consumption in Children
title_sort gut colonization by methanogenic archaea is associated with organic dairy consumption in children
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5344914/
https://www.ncbi.nlm.nih.gov/pubmed/28344572
http://dx.doi.org/10.3389/fmicb.2017.00355
work_keys_str_mv AT vandepoljeroenaa gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT vanbestniels gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT mbakwacatherinea gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT thijscarel gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT savelkoulpaulh gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT artsiljacw gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT hornefmathiasw gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT mommersmonique gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren
AT pendersjohn gutcolonizationbymethanogenicarchaeaisassociatedwithorganicdairyconsumptioninchildren