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Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue

The first Lactobacillus delbrueckii ssp. bulgaricus genome sequence revealed the presence of a very large inverted repeat (IR), a DNA sequence arrangement which thus far seemed inconceivable in a non-manipulated circular bacterial chromosome, at the replication terminus. This intriguing observation...

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Autores principales: El Kafsi, Hela, Loux, Valentin, Mariadassou, Mahendra, Blin, Camille, Chiapello, Hélène, Abraham, Anne-Laure, Maguin, Emmanuelle, van de Guchte, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345009/
https://www.ncbi.nlm.nih.gov/pubmed/28281695
http://dx.doi.org/10.1038/srep44331
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author El Kafsi, Hela
Loux, Valentin
Mariadassou, Mahendra
Blin, Camille
Chiapello, Hélène
Abraham, Anne-Laure
Maguin, Emmanuelle
van de Guchte, Maarten
author_facet El Kafsi, Hela
Loux, Valentin
Mariadassou, Mahendra
Blin, Camille
Chiapello, Hélène
Abraham, Anne-Laure
Maguin, Emmanuelle
van de Guchte, Maarten
author_sort El Kafsi, Hela
collection PubMed
description The first Lactobacillus delbrueckii ssp. bulgaricus genome sequence revealed the presence of a very large inverted repeat (IR), a DNA sequence arrangement which thus far seemed inconceivable in a non-manipulated circular bacterial chromosome, at the replication terminus. This intriguing observation prompted us to investigate if similar IRs could be found in other bacteria. IRs with sizes varying from 38 to 76 kbp were found at the replication terminus of all 5 L. delbrueckii ssp. bulgaricus chromosomes analysed, but in none of 1373 other chromosomes. They represent the first naturally occurring very large IRs detected in circular bacterial genomes. A comparison of the L. bulgaricus replication terminus regions and the corresponding regions without IR in 5 L. delbrueckii ssp. lactis genomes leads us to propose a model for the formation and evolution of the IRs. The DNA sequence data are consistent with a novel model of chromosome rescue after premature replication termination or irreversible chromosome damage near the replication terminus, involving mechanisms analogous to those proposed in the formation of very large IRs in human cancer cells. We postulate that the L. delbrueckii ssp. bulgaricus-specific IRs in different strains derive from a single ancestral IR of at least 93 kbp.
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spelling pubmed-53450092017-03-14 Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue El Kafsi, Hela Loux, Valentin Mariadassou, Mahendra Blin, Camille Chiapello, Hélène Abraham, Anne-Laure Maguin, Emmanuelle van de Guchte, Maarten Sci Rep Article The first Lactobacillus delbrueckii ssp. bulgaricus genome sequence revealed the presence of a very large inverted repeat (IR), a DNA sequence arrangement which thus far seemed inconceivable in a non-manipulated circular bacterial chromosome, at the replication terminus. This intriguing observation prompted us to investigate if similar IRs could be found in other bacteria. IRs with sizes varying from 38 to 76 kbp were found at the replication terminus of all 5 L. delbrueckii ssp. bulgaricus chromosomes analysed, but in none of 1373 other chromosomes. They represent the first naturally occurring very large IRs detected in circular bacterial genomes. A comparison of the L. bulgaricus replication terminus regions and the corresponding regions without IR in 5 L. delbrueckii ssp. lactis genomes leads us to propose a model for the formation and evolution of the IRs. The DNA sequence data are consistent with a novel model of chromosome rescue after premature replication termination or irreversible chromosome damage near the replication terminus, involving mechanisms analogous to those proposed in the formation of very large IRs in human cancer cells. We postulate that the L. delbrueckii ssp. bulgaricus-specific IRs in different strains derive from a single ancestral IR of at least 93 kbp. Nature Publishing Group 2017-03-10 /pmc/articles/PMC5345009/ /pubmed/28281695 http://dx.doi.org/10.1038/srep44331 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
El Kafsi, Hela
Loux, Valentin
Mariadassou, Mahendra
Blin, Camille
Chiapello, Hélène
Abraham, Anne-Laure
Maguin, Emmanuelle
van de Guchte, Maarten
Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title_full Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title_fullStr Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title_full_unstemmed Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title_short Unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
title_sort unprecedented large inverted repeats at the replication terminus of circular bacterial chromosomes suggest a novel mode of chromosome rescue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345009/
https://www.ncbi.nlm.nih.gov/pubmed/28281695
http://dx.doi.org/10.1038/srep44331
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