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Kinetic (15)N-isotope effects on algal growth
Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with curren...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345060/ https://www.ncbi.nlm.nih.gov/pubmed/28281640 http://dx.doi.org/10.1038/srep44181 |
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author | Andriukonis, Eivydas Gorokhova, Elena |
author_facet | Andriukonis, Eivydas Gorokhova, Elena |
author_sort | Andriukonis, Eivydas |
collection | PubMed |
description | Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and empirical knowledge on kinetic isotope effects. Here, we demonstrate profound changes in growth dynamics of the green alga Raphidocelis subcapitata grown in (15)N-enriched media. With increasing (15)N concentration (0.37 to 50 at%), the lag phase increased, whereas maximal growth rate and total yield decreased; moreover, there was a negative relationship between the growth and the lag phase across the treatments. The latter suggests that a trade-off between growth rate and the ability to adapt to the high (15)N environment may exist. Remarkably, the lag-phase response at 3.5 at% (15)N was the shortest and deviated from the overall trend, thus providing partial support to the recently proposed Isotopic Resonance hypothesis, which predicts that certain isotopic composition is particularly favorable for living organisms. These findings confirm the occurrence of KIE in isotopically enriched algae and underline the importance of considering these effects when using stable isotope labeling in field and experimental studies. |
format | Online Article Text |
id | pubmed-5345060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53450602017-03-14 Kinetic (15)N-isotope effects on algal growth Andriukonis, Eivydas Gorokhova, Elena Sci Rep Article Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and empirical knowledge on kinetic isotope effects. Here, we demonstrate profound changes in growth dynamics of the green alga Raphidocelis subcapitata grown in (15)N-enriched media. With increasing (15)N concentration (0.37 to 50 at%), the lag phase increased, whereas maximal growth rate and total yield decreased; moreover, there was a negative relationship between the growth and the lag phase across the treatments. The latter suggests that a trade-off between growth rate and the ability to adapt to the high (15)N environment may exist. Remarkably, the lag-phase response at 3.5 at% (15)N was the shortest and deviated from the overall trend, thus providing partial support to the recently proposed Isotopic Resonance hypothesis, which predicts that certain isotopic composition is particularly favorable for living organisms. These findings confirm the occurrence of KIE in isotopically enriched algae and underline the importance of considering these effects when using stable isotope labeling in field and experimental studies. Nature Publishing Group 2017-03-10 /pmc/articles/PMC5345060/ /pubmed/28281640 http://dx.doi.org/10.1038/srep44181 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Andriukonis, Eivydas Gorokhova, Elena Kinetic (15)N-isotope effects on algal growth |
title | Kinetic (15)N-isotope effects on algal growth |
title_full | Kinetic (15)N-isotope effects on algal growth |
title_fullStr | Kinetic (15)N-isotope effects on algal growth |
title_full_unstemmed | Kinetic (15)N-isotope effects on algal growth |
title_short | Kinetic (15)N-isotope effects on algal growth |
title_sort | kinetic (15)n-isotope effects on algal growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345060/ https://www.ncbi.nlm.nih.gov/pubmed/28281640 http://dx.doi.org/10.1038/srep44181 |
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