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Kinetic (15)N-isotope effects on algal growth

Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with curren...

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Autores principales: Andriukonis, Eivydas, Gorokhova, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345060/
https://www.ncbi.nlm.nih.gov/pubmed/28281640
http://dx.doi.org/10.1038/srep44181
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author Andriukonis, Eivydas
Gorokhova, Elena
author_facet Andriukonis, Eivydas
Gorokhova, Elena
author_sort Andriukonis, Eivydas
collection PubMed
description Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and empirical knowledge on kinetic isotope effects. Here, we demonstrate profound changes in growth dynamics of the green alga Raphidocelis subcapitata grown in (15)N-enriched media. With increasing (15)N concentration (0.37 to 50 at%), the lag phase increased, whereas maximal growth rate and total yield decreased; moreover, there was a negative relationship between the growth and the lag phase across the treatments. The latter suggests that a trade-off between growth rate and the ability to adapt to the high (15)N environment may exist. Remarkably, the lag-phase response at 3.5 at% (15)N was the shortest and deviated from the overall trend, thus providing partial support to the recently proposed Isotopic Resonance hypothesis, which predicts that certain isotopic composition is particularly favorable for living organisms. These findings confirm the occurrence of KIE in isotopically enriched algae and underline the importance of considering these effects when using stable isotope labeling in field and experimental studies.
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spelling pubmed-53450602017-03-14 Kinetic (15)N-isotope effects on algal growth Andriukonis, Eivydas Gorokhova, Elena Sci Rep Article Stable isotope labeling is a standard technique for tracing material transfer in molecular, ecological and biogeochemical studies. The main assumption in this approach is that the enrichment with a heavy isotope has no effect on the organism metabolism and growth, which is not consistent with current theoretical and empirical knowledge on kinetic isotope effects. Here, we demonstrate profound changes in growth dynamics of the green alga Raphidocelis subcapitata grown in (15)N-enriched media. With increasing (15)N concentration (0.37 to 50 at%), the lag phase increased, whereas maximal growth rate and total yield decreased; moreover, there was a negative relationship between the growth and the lag phase across the treatments. The latter suggests that a trade-off between growth rate and the ability to adapt to the high (15)N environment may exist. Remarkably, the lag-phase response at 3.5 at% (15)N was the shortest and deviated from the overall trend, thus providing partial support to the recently proposed Isotopic Resonance hypothesis, which predicts that certain isotopic composition is particularly favorable for living organisms. These findings confirm the occurrence of KIE in isotopically enriched algae and underline the importance of considering these effects when using stable isotope labeling in field and experimental studies. Nature Publishing Group 2017-03-10 /pmc/articles/PMC5345060/ /pubmed/28281640 http://dx.doi.org/10.1038/srep44181 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Andriukonis, Eivydas
Gorokhova, Elena
Kinetic (15)N-isotope effects on algal growth
title Kinetic (15)N-isotope effects on algal growth
title_full Kinetic (15)N-isotope effects on algal growth
title_fullStr Kinetic (15)N-isotope effects on algal growth
title_full_unstemmed Kinetic (15)N-isotope effects on algal growth
title_short Kinetic (15)N-isotope effects on algal growth
title_sort kinetic (15)n-isotope effects on algal growth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345060/
https://www.ncbi.nlm.nih.gov/pubmed/28281640
http://dx.doi.org/10.1038/srep44181
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