Cargando…
Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
BACKGROUND: Cardiotoxicity remains an important concern in drug discovery. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have become an attractive platform to evaluate cardiotoxicity. However, the consistency between human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and hum...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345165/ https://www.ncbi.nlm.nih.gov/pubmed/28279214 http://dx.doi.org/10.1186/s13287-017-0473-x |
_version_ | 1782513664115343360 |
---|---|
author | Zhao, Qi Wang, Xijie Wang, Shuyan Song, Zheng Wang, Jiaxian Ma, Jing |
author_facet | Zhao, Qi Wang, Xijie Wang, Shuyan Song, Zheng Wang, Jiaxian Ma, Jing |
author_sort | Zhao, Qi |
collection | PubMed |
description | BACKGROUND: Cardiotoxicity remains an important concern in drug discovery. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have become an attractive platform to evaluate cardiotoxicity. However, the consistency between human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in prediction of cardiotoxicity has yet to be elucidated. METHODS: Here we screened the toxicities of four representative drugs (E-4031, isoprenaline, quinidine, and haloperidol) using both hESC-CMs and hiPSC-CMs, combined with an impedance-based bioanalytical method. RESULTS: It showed that both hESC-CMs and hiPSC-CMs can recapitulate cardiotoxicity and identify the effects of well-characterized compounds. CONCLUSIONS: The combined platform of hPSC-CMs and an impedance-based bioanalytical method could improve preclinical cardiotoxicity screening, holding great potential for increasing drug development accuracy. |
format | Online Article Text |
id | pubmed-5345165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53451652017-03-14 Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes Zhao, Qi Wang, Xijie Wang, Shuyan Song, Zheng Wang, Jiaxian Ma, Jing Stem Cell Res Ther Research BACKGROUND: Cardiotoxicity remains an important concern in drug discovery. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have become an attractive platform to evaluate cardiotoxicity. However, the consistency between human embryonic stem cell-derived cardiomyocytes (hESC-CMs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in prediction of cardiotoxicity has yet to be elucidated. METHODS: Here we screened the toxicities of four representative drugs (E-4031, isoprenaline, quinidine, and haloperidol) using both hESC-CMs and hiPSC-CMs, combined with an impedance-based bioanalytical method. RESULTS: It showed that both hESC-CMs and hiPSC-CMs can recapitulate cardiotoxicity and identify the effects of well-characterized compounds. CONCLUSIONS: The combined platform of hPSC-CMs and an impedance-based bioanalytical method could improve preclinical cardiotoxicity screening, holding great potential for increasing drug development accuracy. BioMed Central 2017-03-09 /pmc/articles/PMC5345165/ /pubmed/28279214 http://dx.doi.org/10.1186/s13287-017-0473-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhao, Qi Wang, Xijie Wang, Shuyan Song, Zheng Wang, Jiaxian Ma, Jing Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title | Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title_full | Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title_fullStr | Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title_full_unstemmed | Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title_short | Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
title_sort | cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345165/ https://www.ncbi.nlm.nih.gov/pubmed/28279214 http://dx.doi.org/10.1186/s13287-017-0473-x |
work_keys_str_mv | AT zhaoqi cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes AT wangxijie cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes AT wangshuyan cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes AT songzheng cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes AT wangjiaxian cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes AT majing cardiotoxicityevaluationusinghumanembryonicstemcellsandinducedpluripotentstemcellderivedcardiomyocytes |