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Constitutively-stressed yeast strains are high-yielding for recombinant Fps1: implications for the translational regulation of an aquaporin

BACKGROUND: We previously selected four strains of Saccharomyces cerevisiae for their ability to produce the aquaporin Fps1 in sufficient yield for further study. Yields from the yeast strains spt3Δ, srb5Δ, gcn5Δ and yTHCBMS1 (supplemented with 0.5 μg/mL doxycycline) that had been transformed with a...

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Detalles Bibliográficos
Autores principales: Cartwright, Stephanie P., Darby, Richard A. J., Sarkar, Debasmita, Bonander, Nicklas, Gross, Stephane R., Ashe, Mark P., Bill, Roslyn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345182/
https://www.ncbi.nlm.nih.gov/pubmed/28279185
http://dx.doi.org/10.1186/s12934-017-0656-2