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Isolation and characterization of chromosomal markers in Poa pratensis
BACKGROUND: Poa pratensis L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, P. pratensis has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345224/ https://www.ncbi.nlm.nih.gov/pubmed/28293296 http://dx.doi.org/10.1186/s13039-017-0307-7 |
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author | Zhao, Yanyan Yu, Feng Liu, Ruijuan Dou, Quanwen |
author_facet | Zhao, Yanyan Yu, Feng Liu, Ruijuan Dou, Quanwen |
author_sort | Zhao, Yanyan |
collection | PubMed |
description | BACKGROUND: Poa pratensis L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, P. pratensis has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However, currently, no chromosomal markers are available for P. pratensis. RESULTS: Four novel chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined with fluorescence in situ hybridization (FISH) in Poa pratensis. Three tandemly repetitive sequences (PpTR-1, PpTR-2, and PpTR-3) were characterized as subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in PpTR-1, PpTR-2, and PpTR-3, respectively. One tandemly repetitive sequence (PpCR-1) was shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The distribution patterns of PpTR-1, PpTR-2, and PpTR-3 were highly conserved across different P. pratensis cultivars and in the distantly related Poa species, whereas PpCR-1 was conserved across different P. pratensis cultivars, but less conserved across Poa species. CONCLUSION: In this study, we report the identification and characterization of four novel chromosomal markers in P. pratensis. These chromosomal markers are powerful tools for accurate assessment of chromosome count, genomic and phylogenetic analyses, as well as studies of apomixis in P. pratensis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13039-017-0307-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5345224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53452242017-03-14 Isolation and characterization of chromosomal markers in Poa pratensis Zhao, Yanyan Yu, Feng Liu, Ruijuan Dou, Quanwen Mol Cytogenet Research BACKGROUND: Poa pratensis L. is a turf grass and forage crop used worldwide. Being a facultative apomictic species, P. pratensis has a highly variable chromosome number. Chromosomal markers constitute a powerful tool for chromosome identification and for various aspects of genomic research. However, currently, no chromosomal markers are available for P. pratensis. RESULTS: Four novel chromosome markers were isolated from a screen of Cot-1 DNA libraries, combined with fluorescence in situ hybridization (FISH) in Poa pratensis. Three tandemly repetitive sequences (PpTR-1, PpTR-2, and PpTR-3) were characterized as subtelomeric. Monomers of 318 bp, 189 bp and 189 bp were identified in PpTR-1, PpTR-2, and PpTR-3, respectively. One tandemly repetitive sequence (PpCR-1) was shown to be centromeric or pericentromeric, and it had a monomer of 27 bp. The distribution patterns of PpTR-1, PpTR-2, and PpTR-3 were highly conserved across different P. pratensis cultivars and in the distantly related Poa species, whereas PpCR-1 was conserved across different P. pratensis cultivars, but less conserved across Poa species. CONCLUSION: In this study, we report the identification and characterization of four novel chromosomal markers in P. pratensis. These chromosomal markers are powerful tools for accurate assessment of chromosome count, genomic and phylogenetic analyses, as well as studies of apomixis in P. pratensis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13039-017-0307-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-03-09 /pmc/articles/PMC5345224/ /pubmed/28293296 http://dx.doi.org/10.1186/s13039-017-0307-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhao, Yanyan Yu, Feng Liu, Ruijuan Dou, Quanwen Isolation and characterization of chromosomal markers in Poa pratensis |
title | Isolation and characterization of chromosomal markers in Poa pratensis |
title_full | Isolation and characterization of chromosomal markers in Poa pratensis |
title_fullStr | Isolation and characterization of chromosomal markers in Poa pratensis |
title_full_unstemmed | Isolation and characterization of chromosomal markers in Poa pratensis |
title_short | Isolation and characterization of chromosomal markers in Poa pratensis |
title_sort | isolation and characterization of chromosomal markers in poa pratensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345224/ https://www.ncbi.nlm.nih.gov/pubmed/28293296 http://dx.doi.org/10.1186/s13039-017-0307-7 |
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