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Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India

BACKGROUND & OBJECTIVES: Multiple myeloma (MM) is a plasma cell malignancy characterized by cytogenetic heterogeneity. In comparison with conventional karyotyping, fluorescence in situ hybridization (FISH) can efficiently detect various genetic changes in non-cycling plasma cells in 50-90 per ce...

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Autores principales: Kadam Amare, Pratibha S., Jain, Hemani, Nikalje, Shraddha, Sengar, Manju, Menon, Hari, Inamdar, Nitin, Subramanian, P. G., Gujral, Sumeet, Shet, Tanuja, Epari, Sridhar, Nair, Reena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345299/
https://www.ncbi.nlm.nih.gov/pubmed/28256461
http://dx.doi.org/10.4103/0971-5916.200890
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author Kadam Amare, Pratibha S.
Jain, Hemani
Nikalje, Shraddha
Sengar, Manju
Menon, Hari
Inamdar, Nitin
Subramanian, P. G.
Gujral, Sumeet
Shet, Tanuja
Epari, Sridhar
Nair, Reena
author_facet Kadam Amare, Pratibha S.
Jain, Hemani
Nikalje, Shraddha
Sengar, Manju
Menon, Hari
Inamdar, Nitin
Subramanian, P. G.
Gujral, Sumeet
Shet, Tanuja
Epari, Sridhar
Nair, Reena
author_sort Kadam Amare, Pratibha S.
collection PubMed
description BACKGROUND & OBJECTIVES: Multiple myeloma (MM) is a plasma cell malignancy characterized by cytogenetic heterogeneity. In comparison with conventional karyotyping, fluorescence in situ hybridization (FISH) can efficiently detect various genetic changes in non-cycling plasma cells in 50-90 per cent of MM cases. The present study was undertaken in MM patients to evaluate the frequency and clinico-pathological significance of various cytogenetic abnormalities in the Indian population. METHODS: Interphase FISH was applied on purified plasma cells of 475 patients with MM using specific probes. Interphase FISH for 1q gain/1q amplification was performed on a separate group of 250 newly diagnosed MM patients. RESULTS: Low frequency of Δ13 [-13/del(13q)] (32%) and t(11;14) (5%) was observed in our 475 patients probably due to ethnic diversity. Clustering of Δ13, del(17) (p13.1) and IgH translocations in non-hyperdiploidy confirmed prognostic significance of ploidy in MM. t(4;14) and del(17) (p13.1) were high-risk groups due to correlation with high serum β2-microglobulin, increased plasma cells and advanced disease. Hyperdiploidy and t(14;16) were associated with higher age group. In a separate group of 250 patients, 1q amplification [amp(1q)] in combination with Δ13 and/or del(17p) with t(4;14) revealed association with adverse clinico-laboratory features, which confirmed progressive role of amp(1q) with adverse prognostic impact. Amp(1q) was clustered at 1q21 and 1q25 loci. INTERPRETATION & CONCLUSIONS: Based on our findings, it appears that comprehensive analysis of various cytogenetic aberrations by interphase FISH is a powerful strategy being adapted for risk stratification of MM.
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spelling pubmed-53452992017-03-17 Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India Kadam Amare, Pratibha S. Jain, Hemani Nikalje, Shraddha Sengar, Manju Menon, Hari Inamdar, Nitin Subramanian, P. G. Gujral, Sumeet Shet, Tanuja Epari, Sridhar Nair, Reena Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Multiple myeloma (MM) is a plasma cell malignancy characterized by cytogenetic heterogeneity. In comparison with conventional karyotyping, fluorescence in situ hybridization (FISH) can efficiently detect various genetic changes in non-cycling plasma cells in 50-90 per cent of MM cases. The present study was undertaken in MM patients to evaluate the frequency and clinico-pathological significance of various cytogenetic abnormalities in the Indian population. METHODS: Interphase FISH was applied on purified plasma cells of 475 patients with MM using specific probes. Interphase FISH for 1q gain/1q amplification was performed on a separate group of 250 newly diagnosed MM patients. RESULTS: Low frequency of Δ13 [-13/del(13q)] (32%) and t(11;14) (5%) was observed in our 475 patients probably due to ethnic diversity. Clustering of Δ13, del(17) (p13.1) and IgH translocations in non-hyperdiploidy confirmed prognostic significance of ploidy in MM. t(4;14) and del(17) (p13.1) were high-risk groups due to correlation with high serum β2-microglobulin, increased plasma cells and advanced disease. Hyperdiploidy and t(14;16) were associated with higher age group. In a separate group of 250 patients, 1q amplification [amp(1q)] in combination with Δ13 and/or del(17p) with t(4;14) revealed association with adverse clinico-laboratory features, which confirmed progressive role of amp(1q) with adverse prognostic impact. Amp(1q) was clustered at 1q21 and 1q25 loci. INTERPRETATION & CONCLUSIONS: Based on our findings, it appears that comprehensive analysis of various cytogenetic aberrations by interphase FISH is a powerful strategy being adapted for risk stratification of MM. Medknow Publications & Media Pvt Ltd 2016-10 /pmc/articles/PMC5345299/ /pubmed/28256461 http://dx.doi.org/10.4103/0971-5916.200890 Text en Copyright: © 2017 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kadam Amare, Pratibha S.
Jain, Hemani
Nikalje, Shraddha
Sengar, Manju
Menon, Hari
Inamdar, Nitin
Subramanian, P. G.
Gujral, Sumeet
Shet, Tanuja
Epari, Sridhar
Nair, Reena
Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title_full Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title_fullStr Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title_full_unstemmed Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title_short Observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from India
title_sort observation on frequency & clinico-pathological significance of various cytogenetic risk groups in multiple myeloma: an experience from india
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345299/
https://www.ncbi.nlm.nih.gov/pubmed/28256461
http://dx.doi.org/10.4103/0971-5916.200890
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