A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance

In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various “eat me” signals on these cells including Phosphat...

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Autores principales: Banerjee, H, Bazemore, B, Barfield, A, Crummity, D, Krauss, C, Payne, G, Rousch, J, Manglik, V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345489/
https://www.ncbi.nlm.nih.gov/pubmed/28286882
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author Banerjee, H
Bazemore, B
Barfield, A
Crummity, D
Krauss, C
Payne, G
Rousch, J
Manglik, V
author_facet Banerjee, H
Bazemore, B
Barfield, A
Crummity, D
Krauss, C
Payne, G
Rousch, J
Manglik, V
author_sort Banerjee, H
collection PubMed
description In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various “eat me” signals on these cells including Phosphatidyl serine (PtdSer) expression, nitric oxide (NO) signaling and Leukotrine B4 (LTB4) expression and Caspase 3 activation. Investigations were done by fluorescence microscopy techniques, ELISA assay and colorimetric assays using a standard microplate reader and spectrophotometer. Initial results showed all the above mentioned “eat me” signals were significantly decreased in CED 6 knock out cell lines. Therefore CED 6 gene must have a role in cancer cell clearance, pathway involved in the cross talk between CED 6 and other genes in this process is a matter of farther investigation.
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spelling pubmed-53454892017-03-10 A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance Banerjee, H Bazemore, B Barfield, A Crummity, D Krauss, C Payne, G Rousch, J Manglik, V Immunol Disord Immunother Article In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various “eat me” signals on these cells including Phosphatidyl serine (PtdSer) expression, nitric oxide (NO) signaling and Leukotrine B4 (LTB4) expression and Caspase 3 activation. Investigations were done by fluorescence microscopy techniques, ELISA assay and colorimetric assays using a standard microplate reader and spectrophotometer. Initial results showed all the above mentioned “eat me” signals were significantly decreased in CED 6 knock out cell lines. Therefore CED 6 gene must have a role in cancer cell clearance, pathway involved in the cross talk between CED 6 and other genes in this process is a matter of farther investigation. 2016-11-30 2016-12 /pmc/articles/PMC5345489/ /pubmed/28286882 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Banerjee, H
Bazemore, B
Barfield, A
Crummity, D
Krauss, C
Payne, G
Rousch, J
Manglik, V
A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title_full A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title_fullStr A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title_full_unstemmed A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title_short A Study to Investigate the Role of GULP/ CED 6 Gene in “Eat Me” Signaling in Cellular Efferocytosis and Immunosurveillance
title_sort study to investigate the role of gulp/ ced 6 gene in “eat me” signaling in cellular efferocytosis and immunosurveillance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5345489/
https://www.ncbi.nlm.nih.gov/pubmed/28286882
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