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Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications

Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regene...

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Detalles Bibliográficos
Autores principales: Yoshihara, Masahito, Hayashizaki, Yoshihide, Murakawa, Yasuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346115/
https://www.ncbi.nlm.nih.gov/pubmed/27592701
http://dx.doi.org/10.1007/s12015-016-9680-6
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author Yoshihara, Masahito
Hayashizaki, Yoshihide
Murakawa, Yasuhiro
author_facet Yoshihara, Masahito
Hayashizaki, Yoshihide
Murakawa, Yasuhiro
author_sort Yoshihara, Masahito
collection PubMed
description Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regenerative medicine. However, genomic instability of iPSCs has been reported with the advent of high-throughput technologies such as next-generation sequencing. The presence of genetic variations in iPSCs has raised serious safety concerns, hampering the advancement of iPSC-based novel therapies. Here we summarize our current knowledge on genomic instability of iPSCs, with a particular focus on types of genetic variations and their origins. Importantly, it remains elusive whether genetic variations in iPSCs can be an actual risk factor for adverse effects including malignant outgrowth. Furthermore, we discuss novel approaches to generate iPSCs with fewer genetic variations. Lastly, we outline the safety issues and monitoring strategies of iPSCs in clinical settings.
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spelling pubmed-53461152017-03-22 Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications Yoshihara, Masahito Hayashizaki, Yoshihide Murakawa, Yasuhiro Stem Cell Rev Article Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regenerative medicine. However, genomic instability of iPSCs has been reported with the advent of high-throughput technologies such as next-generation sequencing. The presence of genetic variations in iPSCs has raised serious safety concerns, hampering the advancement of iPSC-based novel therapies. Here we summarize our current knowledge on genomic instability of iPSCs, with a particular focus on types of genetic variations and their origins. Importantly, it remains elusive whether genetic variations in iPSCs can be an actual risk factor for adverse effects including malignant outgrowth. Furthermore, we discuss novel approaches to generate iPSCs with fewer genetic variations. Lastly, we outline the safety issues and monitoring strategies of iPSCs in clinical settings. Springer US 2016-09-05 2017 /pmc/articles/PMC5346115/ /pubmed/27592701 http://dx.doi.org/10.1007/s12015-016-9680-6 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Yoshihara, Masahito
Hayashizaki, Yoshihide
Murakawa, Yasuhiro
Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title_full Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title_fullStr Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title_full_unstemmed Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title_short Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
title_sort genomic instability of ipscs: challenges towards their clinical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346115/
https://www.ncbi.nlm.nih.gov/pubmed/27592701
http://dx.doi.org/10.1007/s12015-016-9680-6
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