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Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regene...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346115/ https://www.ncbi.nlm.nih.gov/pubmed/27592701 http://dx.doi.org/10.1007/s12015-016-9680-6 |
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author | Yoshihara, Masahito Hayashizaki, Yoshihide Murakawa, Yasuhiro |
author_facet | Yoshihara, Masahito Hayashizaki, Yoshihide Murakawa, Yasuhiro |
author_sort | Yoshihara, Masahito |
collection | PubMed |
description | Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regenerative medicine. However, genomic instability of iPSCs has been reported with the advent of high-throughput technologies such as next-generation sequencing. The presence of genetic variations in iPSCs has raised serious safety concerns, hampering the advancement of iPSC-based novel therapies. Here we summarize our current knowledge on genomic instability of iPSCs, with a particular focus on types of genetic variations and their origins. Importantly, it remains elusive whether genetic variations in iPSCs can be an actual risk factor for adverse effects including malignant outgrowth. Furthermore, we discuss novel approaches to generate iPSCs with fewer genetic variations. Lastly, we outline the safety issues and monitoring strategies of iPSCs in clinical settings. |
format | Online Article Text |
id | pubmed-5346115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-53461152017-03-22 Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications Yoshihara, Masahito Hayashizaki, Yoshihide Murakawa, Yasuhiro Stem Cell Rev Article Induced pluripotent stem cells (iPSCs) are a type of pluripotent stem cells generated directly from mature cells through the introduction of key transcription factors. iPSCs can be propagated and differentiated into many cell types in the human body, holding enormous potential in the field of regenerative medicine. However, genomic instability of iPSCs has been reported with the advent of high-throughput technologies such as next-generation sequencing. The presence of genetic variations in iPSCs has raised serious safety concerns, hampering the advancement of iPSC-based novel therapies. Here we summarize our current knowledge on genomic instability of iPSCs, with a particular focus on types of genetic variations and their origins. Importantly, it remains elusive whether genetic variations in iPSCs can be an actual risk factor for adverse effects including malignant outgrowth. Furthermore, we discuss novel approaches to generate iPSCs with fewer genetic variations. Lastly, we outline the safety issues and monitoring strategies of iPSCs in clinical settings. Springer US 2016-09-05 2017 /pmc/articles/PMC5346115/ /pubmed/27592701 http://dx.doi.org/10.1007/s12015-016-9680-6 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Yoshihara, Masahito Hayashizaki, Yoshihide Murakawa, Yasuhiro Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title | Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title_full | Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title_fullStr | Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title_full_unstemmed | Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title_short | Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications |
title_sort | genomic instability of ipscs: challenges towards their clinical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346115/ https://www.ncbi.nlm.nih.gov/pubmed/27592701 http://dx.doi.org/10.1007/s12015-016-9680-6 |
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