Cargando…

Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits

The nonselective cation channel transient receptor potential ankyrin 1 (TRPA1) is known to be a key contributor to both somatosensation and pain. Recent studies have implicated TRPA1 in additional physiologic functions and have also suggested that TRPA1 is expressed in nonneuronal tissues. Thus, it...

Descripción completa

Detalles Bibliográficos
Autores principales: Zappia, Katherine J., O’Hara, Crystal L., Moehring, Francie, Kwan, Kelvin Y., Stucky, Cheryl L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Neuroscience 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346175/
https://www.ncbi.nlm.nih.gov/pubmed/28303259
http://dx.doi.org/10.1523/ENEURO.0069-16.2017
_version_ 1782513838183153664
author Zappia, Katherine J.
O’Hara, Crystal L.
Moehring, Francie
Kwan, Kelvin Y.
Stucky, Cheryl L.
author_facet Zappia, Katherine J.
O’Hara, Crystal L.
Moehring, Francie
Kwan, Kelvin Y.
Stucky, Cheryl L.
author_sort Zappia, Katherine J.
collection PubMed
description The nonselective cation channel transient receptor potential ankyrin 1 (TRPA1) is known to be a key contributor to both somatosensation and pain. Recent studies have implicated TRPA1 in additional physiologic functions and have also suggested that TRPA1 is expressed in nonneuronal tissues. Thus, it has become necessary to resolve the importance of TRPA1 expressed in primary sensory neurons, particularly since previous research has largely used global knock-out animals and chemical TRPA1 antagonists. We therefore sought to isolate the physiological relevance of TRPA1 specifically within sensory neurons. To accomplish this, we used Advillin-Cre mice, in which the promoter for Advillin is used to drive expression of Cre recombinase specifically within sensory neurons. These Advillin-Cre mice were crossed with Trpa1(fl/fl) mice to generate sensory neuron-specific Trpa1 knock-out mice. Here, we show that tissue-specific deletion of TRPA1 from sensory neurons produced strong deficits in behavioral sensitivity to mechanical stimulation, while sensitivity to cold and heat stimuli remained intact. The mechanical sensory deficit was incomplete compared to the mechanosensory impairment of TRPA1 global knock-out mice, in line with the incomplete (∼80%) elimination of TRPA1 from sensory neurons in the tissue-specific Advillin-Cre knock-out mice. Equivalent findings were observed in tissue-specific knock-out animals originating from two independently-generated Advillin-Cre lines. As such, our results show that sensory neuron TRPA1 is required for mechanical, but not cold, responsiveness in noninjured skin.
format Online
Article
Text
id pubmed-5346175
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Society for Neuroscience
record_format MEDLINE/PubMed
spelling pubmed-53461752017-03-16 Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits Zappia, Katherine J. O’Hara, Crystal L. Moehring, Francie Kwan, Kelvin Y. Stucky, Cheryl L. eNeuro New Research The nonselective cation channel transient receptor potential ankyrin 1 (TRPA1) is known to be a key contributor to both somatosensation and pain. Recent studies have implicated TRPA1 in additional physiologic functions and have also suggested that TRPA1 is expressed in nonneuronal tissues. Thus, it has become necessary to resolve the importance of TRPA1 expressed in primary sensory neurons, particularly since previous research has largely used global knock-out animals and chemical TRPA1 antagonists. We therefore sought to isolate the physiological relevance of TRPA1 specifically within sensory neurons. To accomplish this, we used Advillin-Cre mice, in which the promoter for Advillin is used to drive expression of Cre recombinase specifically within sensory neurons. These Advillin-Cre mice were crossed with Trpa1(fl/fl) mice to generate sensory neuron-specific Trpa1 knock-out mice. Here, we show that tissue-specific deletion of TRPA1 from sensory neurons produced strong deficits in behavioral sensitivity to mechanical stimulation, while sensitivity to cold and heat stimuli remained intact. The mechanical sensory deficit was incomplete compared to the mechanosensory impairment of TRPA1 global knock-out mice, in line with the incomplete (∼80%) elimination of TRPA1 from sensory neurons in the tissue-specific Advillin-Cre knock-out mice. Equivalent findings were observed in tissue-specific knock-out animals originating from two independently-generated Advillin-Cre lines. As such, our results show that sensory neuron TRPA1 is required for mechanical, but not cold, responsiveness in noninjured skin. Society for Neuroscience 2017-03-13 /pmc/articles/PMC5346175/ /pubmed/28303259 http://dx.doi.org/10.1523/ENEURO.0069-16.2017 Text en Copyright © 2017 Zappia et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle New Research
Zappia, Katherine J.
O’Hara, Crystal L.
Moehring, Francie
Kwan, Kelvin Y.
Stucky, Cheryl L.
Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title_full Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title_fullStr Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title_full_unstemmed Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title_short Sensory Neuron-Specific Deletion of TRPA1 Results in Mechanical Cutaneous Sensory Deficits
title_sort sensory neuron-specific deletion of trpa1 results in mechanical cutaneous sensory deficits
topic New Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346175/
https://www.ncbi.nlm.nih.gov/pubmed/28303259
http://dx.doi.org/10.1523/ENEURO.0069-16.2017
work_keys_str_mv AT zappiakatherinej sensoryneuronspecificdeletionoftrpa1resultsinmechanicalcutaneoussensorydeficits
AT oharacrystall sensoryneuronspecificdeletionoftrpa1resultsinmechanicalcutaneoussensorydeficits
AT moehringfrancie sensoryneuronspecificdeletionoftrpa1resultsinmechanicalcutaneoussensorydeficits
AT kwankelviny sensoryneuronspecificdeletionoftrpa1resultsinmechanicalcutaneoussensorydeficits
AT stuckycheryll sensoryneuronspecificdeletionoftrpa1resultsinmechanicalcutaneoussensorydeficits