Cargando…

Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele

The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythm...

Descripción completa

Detalles Bibliográficos
Autores principales: Cho, Eunjoo, Lee, Euna, Kim, Eun Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346317/
https://www.ncbi.nlm.nih.gov/pubmed/27756446
http://dx.doi.org/10.5483/BMBRep.2016.49.11.178
_version_ 1782513863474806784
author Cho, Eunjoo
Lee, Euna
Kim, Eun Young
author_facet Cho, Eunjoo
Lee, Euna
Kim, Eun Young
author_sort Cho, Eunjoo
collection PubMed
description The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657–707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in Clk(out) genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons (LN(v)s) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues.
format Online
Article
Text
id pubmed-5346317
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Korean Society for Biochemistry and Molecular Biology
record_format MEDLINE/PubMed
spelling pubmed-53463172017-04-06 Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele Cho, Eunjoo Lee, Euna Kim, Eun Young BMB Rep Perspective The circadian clock system enables organisms to anticipate the rhythmic environmental changes and to manifest behavior and physiology at advantageous times of the day. Transcriptional/translational feedback loop (TTFL) is the basic feature of the eukaryotic circadian clock and is based on the rhythmic association of circadian transcriptional activator and repressor. In Drosophila, repression of dCLOCK/CYCLE (dCLK/CYC) mediated transcription by PERIOD (PER) is critical for inducing circadian rhythms of gene expression. Pacemaker neurons in the brain control specific circadian behaviors upon environmental timing cues such as light and temperature cycle. We show that amino acids 657–707 of dCLK are important for the transcriptional activation and the association with PER both in vitro and in vivo. Flies expressing dCLK lacking AA657-707 in Clk(out) genetic background, homologous to the mouse Clock allele where exon 19 region is deleted, display pacemaker-neuron-dependent perturbation of the molecular clockwork. The molecular rhythms in light-cycle-sensitive pacemaker neurons such as ventral lateral neurons (LN(v)s) were significantly disrupted, but those in temperature-cycle-sensitive pacemaker neurons such as dorsal neurons (DNs) were robust. Our results suggest that the dCLK-controlled TTFL diversify in a pacemaker-neuron-dependent manner which may contribute to specific functions such as different sensitivities to entraining cues. Korean Society for Biochemistry and Molecular Biology 2016 2016-11-30 /pmc/articles/PMC5346317/ /pubmed/27756446 http://dx.doi.org/10.5483/BMBRep.2016.49.11.178 Text en Copyright © 2016 by the The Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Perspective
Cho, Eunjoo
Lee, Euna
Kim, Eun Young
Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title_full Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title_fullStr Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title_full_unstemmed Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title_short Diversification of the molecular clockwork for tissue-specific function: insight from a novel Drosophila Clock mutant homologous to a mouse Clock allele
title_sort diversification of the molecular clockwork for tissue-specific function: insight from a novel drosophila clock mutant homologous to a mouse clock allele
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346317/
https://www.ncbi.nlm.nih.gov/pubmed/27756446
http://dx.doi.org/10.5483/BMBRep.2016.49.11.178
work_keys_str_mv AT choeunjoo diversificationofthemolecularclockworkfortissuespecificfunctioninsightfromanoveldrosophilaclockmutanthomologoustoamouseclockallele
AT leeeuna diversificationofthemolecularclockworkfortissuespecificfunctioninsightfromanoveldrosophilaclockmutanthomologoustoamouseclockallele
AT kimeunyoung diversificationofthemolecularclockworkfortissuespecificfunctioninsightfromanoveldrosophilaclockmutanthomologoustoamouseclockallele