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Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count

Background. Dendritic cells could be involved in immune surveillance of highly immunogenic tumors such as melanoma. Their role in the progression melanocytic nevi to melanoma is however a matter of controversy. Methods. The number of dendritic cells within epidermis, in peritumoral zone, and within...

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Autores principales: Dyduch, Grzegorz, Tyrak, Katarzyna Ewa, Glajcar, Anna, Szpor, Joanna, Ulatowska-Białas, Magdalena, Okoń, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346357/
https://www.ncbi.nlm.nih.gov/pubmed/28331853
http://dx.doi.org/10.1155/2017/6803756
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author Dyduch, Grzegorz
Tyrak, Katarzyna Ewa
Glajcar, Anna
Szpor, Joanna
Ulatowska-Białas, Magdalena
Okoń, Krzysztof
author_facet Dyduch, Grzegorz
Tyrak, Katarzyna Ewa
Glajcar, Anna
Szpor, Joanna
Ulatowska-Białas, Magdalena
Okoń, Krzysztof
author_sort Dyduch, Grzegorz
collection PubMed
description Background. Dendritic cells could be involved in immune surveillance of highly immunogenic tumors such as melanoma. Their role in the progression melanocytic nevi to melanoma is however a matter of controversy. Methods. The number of dendritic cells within epidermis, in peritumoral zone, and within the lesion was counted on slides immunohistochemically stained for CD1a, CD1c, DC-LAMP, and DC-SIGN in 21 of dysplastic nevi, 27 in situ melanomas, and 21 invasive melanomas. Results. We found a significant difference in the density of intraepidermal CD1c+ cells between the examined lesions; the mean CD1c cell count was 7.00/mm(2) for invasive melanomas, 2.94 for in situ melanomas, and 13.35 for dysplastic nevi. The differences between dysplastic nevi and melanoma in situ as well as between dysplastic nevi and invasive melanoma were significant. There was no correlation in number of positively stained cells between epidermis and dermis. We did not observe any intraepidermal DC-LAMP+ cells neither in melanoma in situ nor in invasive melanoma as well as any intraepidermal DC-SIGN+ cells in dysplastic nevi. Conclusion. It was shown that the number of dendritic cells differs between dysplastic nevi, in situ melanomas, and invasive melanomas. This could eventually suggest their participation in the development of melanoma.
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spelling pubmed-53463572017-03-22 Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count Dyduch, Grzegorz Tyrak, Katarzyna Ewa Glajcar, Anna Szpor, Joanna Ulatowska-Białas, Magdalena Okoń, Krzysztof Biomed Res Int Research Article Background. Dendritic cells could be involved in immune surveillance of highly immunogenic tumors such as melanoma. Their role in the progression melanocytic nevi to melanoma is however a matter of controversy. Methods. The number of dendritic cells within epidermis, in peritumoral zone, and within the lesion was counted on slides immunohistochemically stained for CD1a, CD1c, DC-LAMP, and DC-SIGN in 21 of dysplastic nevi, 27 in situ melanomas, and 21 invasive melanomas. Results. We found a significant difference in the density of intraepidermal CD1c+ cells between the examined lesions; the mean CD1c cell count was 7.00/mm(2) for invasive melanomas, 2.94 for in situ melanomas, and 13.35 for dysplastic nevi. The differences between dysplastic nevi and melanoma in situ as well as between dysplastic nevi and invasive melanoma were significant. There was no correlation in number of positively stained cells between epidermis and dermis. We did not observe any intraepidermal DC-LAMP+ cells neither in melanoma in situ nor in invasive melanoma as well as any intraepidermal DC-SIGN+ cells in dysplastic nevi. Conclusion. It was shown that the number of dendritic cells differs between dysplastic nevi, in situ melanomas, and invasive melanomas. This could eventually suggest their participation in the development of melanoma. Hindawi Publishing Corporation 2017 2017-02-26 /pmc/articles/PMC5346357/ /pubmed/28331853 http://dx.doi.org/10.1155/2017/6803756 Text en Copyright © 2017 Grzegorz Dyduch et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dyduch, Grzegorz
Tyrak, Katarzyna Ewa
Glajcar, Anna
Szpor, Joanna
Ulatowska-Białas, Magdalena
Okoń, Krzysztof
Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title_full Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title_fullStr Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title_full_unstemmed Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title_short Melanomas and Dysplastic Nevi Differ in Epidermal CD1c+ Dendritic Cell Count
title_sort melanomas and dysplastic nevi differ in epidermal cd1c+ dendritic cell count
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346357/
https://www.ncbi.nlm.nih.gov/pubmed/28331853
http://dx.doi.org/10.1155/2017/6803756
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