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A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments

Myeloid-derived suppressor cells (MDSCs) are a heterogeneous subset of cells that expands dramatically in many disease states and can suppress T-cell responses. MDSCs mainly include monocytic and granulocytic subpopulations that can be distinguished in mice by the expression of Ly6G and Ly6C cell su...

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Autores principales: Jia, Bei, Zhao, Chenchen, Li, Guoli, Kong, Yaxian, Ma, Yaluan, Wang, Qiuping, Wang, Beibei, Zeng, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346402/
https://www.ncbi.nlm.nih.gov/pubmed/28337051
http://dx.doi.org/10.1155/2017/7521701
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author Jia, Bei
Zhao, Chenchen
Li, Guoli
Kong, Yaxian
Ma, Yaluan
Wang, Qiuping
Wang, Beibei
Zeng, Hui
author_facet Jia, Bei
Zhao, Chenchen
Li, Guoli
Kong, Yaxian
Ma, Yaluan
Wang, Qiuping
Wang, Beibei
Zeng, Hui
author_sort Jia, Bei
collection PubMed
description Myeloid-derived suppressor cells (MDSCs) are a heterogeneous subset of cells that expands dramatically in many disease states and can suppress T-cell responses. MDSCs mainly include monocytic and granulocytic subpopulations that can be distinguished in mice by the expression of Ly6G and Ly6C cell surface markers. This identification system has been validated in experimental tumor models, but not in models of inflammation-associated conditions such as sepsis. We challenged growth factor independent 1 transcription repressor green fluorescent protein (Gfi1:GFP) knock-in reporter mice with cecal ligation and puncture surgery and found that CD11b(+)Ly6G(low)Ly6C(high) MDSCs in this sepsis model comprised both monocytic and granulocytic MDSCs. The evidence that conventional Ly6G/Ly6C marker analysis may not be suited to study of inflammation-induced MDSCs led to the development of a novel strategy of distinguishing granulocytic MDSCs from monocytic MDSCs in septic mice by expression of CD48. Application of this novel model should help achieve a more accurate understanding of the inflammation-induced MDSC activity.
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spelling pubmed-53464022017-03-23 A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments Jia, Bei Zhao, Chenchen Li, Guoli Kong, Yaxian Ma, Yaluan Wang, Qiuping Wang, Beibei Zeng, Hui Mediators Inflamm Research Article Myeloid-derived suppressor cells (MDSCs) are a heterogeneous subset of cells that expands dramatically in many disease states and can suppress T-cell responses. MDSCs mainly include monocytic and granulocytic subpopulations that can be distinguished in mice by the expression of Ly6G and Ly6C cell surface markers. This identification system has been validated in experimental tumor models, but not in models of inflammation-associated conditions such as sepsis. We challenged growth factor independent 1 transcription repressor green fluorescent protein (Gfi1:GFP) knock-in reporter mice with cecal ligation and puncture surgery and found that CD11b(+)Ly6G(low)Ly6C(high) MDSCs in this sepsis model comprised both monocytic and granulocytic MDSCs. The evidence that conventional Ly6G/Ly6C marker analysis may not be suited to study of inflammation-induced MDSCs led to the development of a novel strategy of distinguishing granulocytic MDSCs from monocytic MDSCs in septic mice by expression of CD48. Application of this novel model should help achieve a more accurate understanding of the inflammation-induced MDSC activity. Hindawi Publishing Corporation 2017 2017-02-26 /pmc/articles/PMC5346402/ /pubmed/28337051 http://dx.doi.org/10.1155/2017/7521701 Text en Copyright © 2017 Bei Jia et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jia, Bei
Zhao, Chenchen
Li, Guoli
Kong, Yaxian
Ma, Yaluan
Wang, Qiuping
Wang, Beibei
Zeng, Hui
A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title_full A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title_fullStr A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title_full_unstemmed A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title_short A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments
title_sort novel cd48-based analysis of sepsis-induced mouse myeloid-derived suppressor cell compartments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346402/
https://www.ncbi.nlm.nih.gov/pubmed/28337051
http://dx.doi.org/10.1155/2017/7521701
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