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The interaction of fluorescent nanodiamond probes with cellular media

Fluorescent nanodiamonds (FNDs) are promising tools to image cells, bioanalytes and physical quantities such as temperature, pressure, and electric or magnetic fields with nanometer resolution. To exploit their potential for intracellular applications, the FNDs have to be brought into contact with c...

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Autores principales: Hemelaar, Simon R., Nagl, Andreas, Bigot, François, Rodríguez-García, Melissa M., de Vries, Marcel P., Chipaux, Mayeul, Schirhagl, Romana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346409/
https://www.ncbi.nlm.nih.gov/pubmed/28344361
http://dx.doi.org/10.1007/s00604-017-2086-6
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author Hemelaar, Simon R.
Nagl, Andreas
Bigot, François
Rodríguez-García, Melissa M.
de Vries, Marcel P.
Chipaux, Mayeul
Schirhagl, Romana
author_facet Hemelaar, Simon R.
Nagl, Andreas
Bigot, François
Rodríguez-García, Melissa M.
de Vries, Marcel P.
Chipaux, Mayeul
Schirhagl, Romana
author_sort Hemelaar, Simon R.
collection PubMed
description Fluorescent nanodiamonds (FNDs) are promising tools to image cells, bioanalytes and physical quantities such as temperature, pressure, and electric or magnetic fields with nanometer resolution. To exploit their potential for intracellular applications, the FNDs have to be brought into contact with cell culture media. The interactions between the medium and the diamonds crucially influence sensitivity as well as the ability to enter cells. The authors demonstrate that certain proteins and salts spontaneously adhere to the FNDs and may cause aggregation. This is a first investigation on the fundamental questions on how (a) FNDs interact with the medium, and (b) which proteins and salts are being attracted. A differentiation between strongly binding and weakly binding proteins is made. Not all proteins participate in the formation of FND aggregates. Surprisingly, some main components in the medium seem to play no role in aggregation. Simple strategies to prevent aggregation are discussed. These include adding the proteins, which are naturally present in the cell culture to the diamonds first and then inserting them in the full medium. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00604-017-2086-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-53464092017-03-24 The interaction of fluorescent nanodiamond probes with cellular media Hemelaar, Simon R. Nagl, Andreas Bigot, François Rodríguez-García, Melissa M. de Vries, Marcel P. Chipaux, Mayeul Schirhagl, Romana Mikrochim Acta Original Paper Fluorescent nanodiamonds (FNDs) are promising tools to image cells, bioanalytes and physical quantities such as temperature, pressure, and electric or magnetic fields with nanometer resolution. To exploit their potential for intracellular applications, the FNDs have to be brought into contact with cell culture media. The interactions between the medium and the diamonds crucially influence sensitivity as well as the ability to enter cells. The authors demonstrate that certain proteins and salts spontaneously adhere to the FNDs and may cause aggregation. This is a first investigation on the fundamental questions on how (a) FNDs interact with the medium, and (b) which proteins and salts are being attracted. A differentiation between strongly binding and weakly binding proteins is made. Not all proteins participate in the formation of FND aggregates. Surprisingly, some main components in the medium seem to play no role in aggregation. Simple strategies to prevent aggregation are discussed. These include adding the proteins, which are naturally present in the cell culture to the diamonds first and then inserting them in the full medium. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00604-017-2086-6) contains supplementary material, which is available to authorized users. Springer Vienna 2017-01-27 2017 /pmc/articles/PMC5346409/ /pubmed/28344361 http://dx.doi.org/10.1007/s00604-017-2086-6 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Hemelaar, Simon R.
Nagl, Andreas
Bigot, François
Rodríguez-García, Melissa M.
de Vries, Marcel P.
Chipaux, Mayeul
Schirhagl, Romana
The interaction of fluorescent nanodiamond probes with cellular media
title The interaction of fluorescent nanodiamond probes with cellular media
title_full The interaction of fluorescent nanodiamond probes with cellular media
title_fullStr The interaction of fluorescent nanodiamond probes with cellular media
title_full_unstemmed The interaction of fluorescent nanodiamond probes with cellular media
title_short The interaction of fluorescent nanodiamond probes with cellular media
title_sort interaction of fluorescent nanodiamond probes with cellular media
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346409/
https://www.ncbi.nlm.nih.gov/pubmed/28344361
http://dx.doi.org/10.1007/s00604-017-2086-6
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