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A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells
A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast an...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346650/ https://www.ncbi.nlm.nih.gov/pubmed/27191997 http://dx.doi.org/10.18632/oncotarget.9382 |
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author | Kong, Jing Luo, Yong Jin, Dong An, Fan Zhang, Wenyuan Liu, Lilu Li, Jiao Fang, Shimeng Li, Xiaojie Yang, Xuesong Lin, Bingcheng Liu, Tingjiao |
author_facet | Kong, Jing Luo, Yong Jin, Dong An, Fan Zhang, Wenyuan Liu, Lilu Li, Jiao Fang, Shimeng Li, Xiaojie Yang, Xuesong Lin, Bingcheng Liu, Tingjiao |
author_sort | Kong, Jing |
collection | PubMed |
description | A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models. |
format | Online Article Text |
id | pubmed-5346650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53466502017-03-30 A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells Kong, Jing Luo, Yong Jin, Dong An, Fan Zhang, Wenyuan Liu, Lilu Li, Jiao Fang, Shimeng Li, Xiaojie Yang, Xuesong Lin, Bingcheng Liu, Tingjiao Oncotarget Research Paper A biomimetic microsystem might compensate costly and time-consuming animal metastatic models. Herein we developed a biomimetic microfluidic model to study cancer metastasis. Primary cells isolated from different organs were cultured on the microlfuidic model to represent individual organs. Breast and salivary gland cancer cells were driven to flow over primary cell culture chambers, mimicking dynamic adhesion of circulating tumor cells (CTCs) to endothelium in vivo. These flowing artificial CTCs showed different metastatic potentials to lung on the microfluidic model. The traditional nude mouse model of lung metastasis was performed to investigate the physiological similarity of the microfluidic model to animal models. It was found that the metastatic potential of different cancer cells assessed by the microfluidic model was in agreement with that assessed by the nude mouse model. Furthermore, it was demonstrated that the metastatic inhibitor AMD3100 inhibited lung metastasis effectively in both the microfluidic model and the nude mouse model. Then the microfluidic model was used to mimick liver and bone metastasis of CTCs and confirm the potential for research of multiple-organ metastasis. Thus, the metastasis of CTCs to different organs was reconstituted on the microfluidic model. It may expand the capabilities of traditional cell culture models, providing a low-cost, time-saving, and rapid alternative to animal models. Impact Journals LLC 2016-05-15 /pmc/articles/PMC5346650/ /pubmed/27191997 http://dx.doi.org/10.18632/oncotarget.9382 Text en Copyright: © 2016 Kong et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Kong, Jing Luo, Yong Jin, Dong An, Fan Zhang, Wenyuan Liu, Lilu Li, Jiao Fang, Shimeng Li, Xiaojie Yang, Xuesong Lin, Bingcheng Liu, Tingjiao A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title | A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title_full | A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title_fullStr | A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title_full_unstemmed | A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title_short | A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
title_sort | novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346650/ https://www.ncbi.nlm.nih.gov/pubmed/27191997 http://dx.doi.org/10.18632/oncotarget.9382 |
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