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The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer

Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and pro...

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Autores principales: Zhang, Mingdi, Gong, Wei, Zuo, Bin, Chu, Bingfeng, Tang, Zhaohui, Zhang, Yong, Yang, Yong, Zhou, Di, Weng, Mingzhe, Qin, Yiyu, Ma, Mingzhe, Jiang, Alex, Ma, Fei, Quan, Zhiwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346666/
https://www.ncbi.nlm.nih.gov/pubmed/27769047
http://dx.doi.org/10.18632/oncotarget.12693
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author Zhang, Mingdi
Gong, Wei
Zuo, Bin
Chu, Bingfeng
Tang, Zhaohui
Zhang, Yong
Yang, Yong
Zhou, Di
Weng, Mingzhe
Qin, Yiyu
Ma, Mingzhe
Jiang, Alex
Ma, Fei
Quan, Zhiwei
author_facet Zhang, Mingdi
Gong, Wei
Zuo, Bin
Chu, Bingfeng
Tang, Zhaohui
Zhang, Yong
Yang, Yong
Zhou, Di
Weng, Mingzhe
Qin, Yiyu
Ma, Mingzhe
Jiang, Alex
Ma, Fei
Quan, Zhiwei
author_sort Zhang, Mingdi
collection PubMed
description Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and prognostic significance of interleukin-6 (IL-6) -induced miRNAs in the development of GBC. We identify that inflammatory cytokine, IL-6 promotes proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of GBC both in vitro and in vivo. Among all the changed miRNAs in miRNA profiling, miR-33a expression was significantly decreased in IL-6 treated GBC cell lines, as well as in GBC tissues compared with case-matched normal tissues and cholecystitis tissues. In turn, miR-33a suppresses IL-6−induced tumor metastasis by directly binding Twist which was identified as an EMT marker. High expression of miR-33a suppressed xenograft tumor growth and dissemination in nude mice. The downregulation of miR-33a was closely associated with advanced clinical stage, lymph node metastasis, and poor clinical outcomes in patients with GBC. miR-33a acts as a tumor suppressor miRNA in GBC progression and may be considered for the development of potential therapeutics against GBC.
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spelling pubmed-53466662017-03-30 The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer Zhang, Mingdi Gong, Wei Zuo, Bin Chu, Bingfeng Tang, Zhaohui Zhang, Yong Yang, Yong Zhou, Di Weng, Mingzhe Qin, Yiyu Ma, Mingzhe Jiang, Alex Ma, Fei Quan, Zhiwei Oncotarget Research Paper Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and prognostic significance of interleukin-6 (IL-6) -induced miRNAs in the development of GBC. We identify that inflammatory cytokine, IL-6 promotes proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of GBC both in vitro and in vivo. Among all the changed miRNAs in miRNA profiling, miR-33a expression was significantly decreased in IL-6 treated GBC cell lines, as well as in GBC tissues compared with case-matched normal tissues and cholecystitis tissues. In turn, miR-33a suppresses IL-6−induced tumor metastasis by directly binding Twist which was identified as an EMT marker. High expression of miR-33a suppressed xenograft tumor growth and dissemination in nude mice. The downregulation of miR-33a was closely associated with advanced clinical stage, lymph node metastasis, and poor clinical outcomes in patients with GBC. miR-33a acts as a tumor suppressor miRNA in GBC progression and may be considered for the development of potential therapeutics against GBC. Impact Journals LLC 2016-10-15 /pmc/articles/PMC5346666/ /pubmed/27769047 http://dx.doi.org/10.18632/oncotarget.12693 Text en Copyright: © 2016 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhang, Mingdi
Gong, Wei
Zuo, Bin
Chu, Bingfeng
Tang, Zhaohui
Zhang, Yong
Yang, Yong
Zhou, Di
Weng, Mingzhe
Qin, Yiyu
Ma, Mingzhe
Jiang, Alex
Ma, Fei
Quan, Zhiwei
The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title_full The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title_fullStr The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title_full_unstemmed The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title_short The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
title_sort microrna mir-33a suppresses il-6-induced tumor progression by binding twist in gallbladder cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346666/
https://www.ncbi.nlm.nih.gov/pubmed/27769047
http://dx.doi.org/10.18632/oncotarget.12693
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