Cargando…
The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer
Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and pro...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346666/ https://www.ncbi.nlm.nih.gov/pubmed/27769047 http://dx.doi.org/10.18632/oncotarget.12693 |
_version_ | 1782513924360372224 |
---|---|
author | Zhang, Mingdi Gong, Wei Zuo, Bin Chu, Bingfeng Tang, Zhaohui Zhang, Yong Yang, Yong Zhou, Di Weng, Mingzhe Qin, Yiyu Ma, Mingzhe Jiang, Alex Ma, Fei Quan, Zhiwei |
author_facet | Zhang, Mingdi Gong, Wei Zuo, Bin Chu, Bingfeng Tang, Zhaohui Zhang, Yong Yang, Yong Zhou, Di Weng, Mingzhe Qin, Yiyu Ma, Mingzhe Jiang, Alex Ma, Fei Quan, Zhiwei |
author_sort | Zhang, Mingdi |
collection | PubMed |
description | Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and prognostic significance of interleukin-6 (IL-6) -induced miRNAs in the development of GBC. We identify that inflammatory cytokine, IL-6 promotes proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of GBC both in vitro and in vivo. Among all the changed miRNAs in miRNA profiling, miR-33a expression was significantly decreased in IL-6 treated GBC cell lines, as well as in GBC tissues compared with case-matched normal tissues and cholecystitis tissues. In turn, miR-33a suppresses IL-6−induced tumor metastasis by directly binding Twist which was identified as an EMT marker. High expression of miR-33a suppressed xenograft tumor growth and dissemination in nude mice. The downregulation of miR-33a was closely associated with advanced clinical stage, lymph node metastasis, and poor clinical outcomes in patients with GBC. miR-33a acts as a tumor suppressor miRNA in GBC progression and may be considered for the development of potential therapeutics against GBC. |
format | Online Article Text |
id | pubmed-5346666 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53466662017-03-30 The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer Zhang, Mingdi Gong, Wei Zuo, Bin Chu, Bingfeng Tang, Zhaohui Zhang, Yong Yang, Yong Zhou, Di Weng, Mingzhe Qin, Yiyu Ma, Mingzhe Jiang, Alex Ma, Fei Quan, Zhiwei Oncotarget Research Paper Cytokine is a key molecular link between chronic inflammation and gallbladder cancer (GBC) progression. The potential mechanism of cytokine-associated modulation of microRNAs (miRNAs) expression in GBC progression is not fully understood. In this study, we investigated the biological effects and prognostic significance of interleukin-6 (IL-6) -induced miRNAs in the development of GBC. We identify that inflammatory cytokine, IL-6 promotes proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of GBC both in vitro and in vivo. Among all the changed miRNAs in miRNA profiling, miR-33a expression was significantly decreased in IL-6 treated GBC cell lines, as well as in GBC tissues compared with case-matched normal tissues and cholecystitis tissues. In turn, miR-33a suppresses IL-6−induced tumor metastasis by directly binding Twist which was identified as an EMT marker. High expression of miR-33a suppressed xenograft tumor growth and dissemination in nude mice. The downregulation of miR-33a was closely associated with advanced clinical stage, lymph node metastasis, and poor clinical outcomes in patients with GBC. miR-33a acts as a tumor suppressor miRNA in GBC progression and may be considered for the development of potential therapeutics against GBC. Impact Journals LLC 2016-10-15 /pmc/articles/PMC5346666/ /pubmed/27769047 http://dx.doi.org/10.18632/oncotarget.12693 Text en Copyright: © 2016 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhang, Mingdi Gong, Wei Zuo, Bin Chu, Bingfeng Tang, Zhaohui Zhang, Yong Yang, Yong Zhou, Di Weng, Mingzhe Qin, Yiyu Ma, Mingzhe Jiang, Alex Ma, Fei Quan, Zhiwei The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title | The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title_full | The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title_fullStr | The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title_full_unstemmed | The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title_short | The microRNA miR-33a suppresses IL-6-induced tumor progression by binding Twist in gallbladder cancer |
title_sort | microrna mir-33a suppresses il-6-induced tumor progression by binding twist in gallbladder cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346666/ https://www.ncbi.nlm.nih.gov/pubmed/27769047 http://dx.doi.org/10.18632/oncotarget.12693 |
work_keys_str_mv | AT zhangmingdi themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT gongwei themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zuobin themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT chubingfeng themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT tangzhaohui themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zhangyong themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT yangyong themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zhoudi themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT wengmingzhe themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT qinyiyu themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT mamingzhe themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT jiangalex themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT mafei themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT quanzhiwei themicrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zhangmingdi micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT gongwei micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zuobin micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT chubingfeng micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT tangzhaohui micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zhangyong micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT yangyong micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT zhoudi micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT wengmingzhe micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT qinyiyu micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT mamingzhe micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT jiangalex micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT mafei micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer AT quanzhiwei micrornamir33asuppressesil6inducedtumorprogressionbybindingtwistingallbladdercancer |