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Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin
VTo investigate the effect of nuclear transcription factor Nrf2 on the transcription of Ferroportin (FPN) in prostate cancer cells, and the regulation mechanisms of FPN on cell viability, migration and apoptosis of prostate cancer cells. Empty vectors, pEGFPC1-Nrf2, pEGFPC1-FPN, Si-FPN and Si-Nrf2 w...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346678/ https://www.ncbi.nlm.nih.gov/pubmed/27788496 http://dx.doi.org/10.18632/oncotarget.12860 |
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author | Xue, Dong Zhou, Cuixing Shi, Yunbo Lu, Hao Xu, Renfang He, Xiaozhou |
author_facet | Xue, Dong Zhou, Cuixing Shi, Yunbo Lu, Hao Xu, Renfang He, Xiaozhou |
author_sort | Xue, Dong |
collection | PubMed |
description | VTo investigate the effect of nuclear transcription factor Nrf2 on the transcription of Ferroportin (FPN) in prostate cancer cells, and the regulation mechanisms of FPN on cell viability, migration and apoptosis of prostate cancer cells. Empty vectors, pEGFPC1-Nrf2, pEGFPC1-FPN, Si-FPN and Si-Nrf2 were transfected into prostate cancer cell line PC3. The expression of mRNA and protein were measured by real time-PCR (RT-PCR) and western blot. Cell viability, migration, cycle and apoptosis were tested by CCK-8 assay, wound healing and flow cytometry, respectively. The interaction between FPN and Nrf2 was confirmed by chromatin immunoprecipitation (CHIP) assay. The viability, migration and mitosis of PC3 cells could be repressed by over-expressed FPN, with decreased intracellular ferritin. The CHIP assay demonstrated that Nrf2 is one transcription factor of FPN and promotes its transcription. With the increase of Nrf2 in PC3 cells, the viability, migration ability and concentration of ferritin were suppressed, while the apoptosis rate was increased. The above effects were counteracted by down-regulating FPN. FPN could inhibit the prostate cancer cell viability, migration and mitosis, which is also related to a decrease of intracellular ferritin content. In conclusion, Nrf2 suppresses prostate cancer cells viability, migration, and mitosis through upregulating FPN. |
format | Online Article Text |
id | pubmed-5346678 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53466782017-03-30 Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin Xue, Dong Zhou, Cuixing Shi, Yunbo Lu, Hao Xu, Renfang He, Xiaozhou Oncotarget Research Paper VTo investigate the effect of nuclear transcription factor Nrf2 on the transcription of Ferroportin (FPN) in prostate cancer cells, and the regulation mechanisms of FPN on cell viability, migration and apoptosis of prostate cancer cells. Empty vectors, pEGFPC1-Nrf2, pEGFPC1-FPN, Si-FPN and Si-Nrf2 were transfected into prostate cancer cell line PC3. The expression of mRNA and protein were measured by real time-PCR (RT-PCR) and western blot. Cell viability, migration, cycle and apoptosis were tested by CCK-8 assay, wound healing and flow cytometry, respectively. The interaction between FPN and Nrf2 was confirmed by chromatin immunoprecipitation (CHIP) assay. The viability, migration and mitosis of PC3 cells could be repressed by over-expressed FPN, with decreased intracellular ferritin. The CHIP assay demonstrated that Nrf2 is one transcription factor of FPN and promotes its transcription. With the increase of Nrf2 in PC3 cells, the viability, migration ability and concentration of ferritin were suppressed, while the apoptosis rate was increased. The above effects were counteracted by down-regulating FPN. FPN could inhibit the prostate cancer cell viability, migration and mitosis, which is also related to a decrease of intracellular ferritin content. In conclusion, Nrf2 suppresses prostate cancer cells viability, migration, and mitosis through upregulating FPN. Impact Journals LLC 2016-10-24 /pmc/articles/PMC5346678/ /pubmed/27788496 http://dx.doi.org/10.18632/oncotarget.12860 Text en Copyright: © 2016 Xue et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xue, Dong Zhou, Cuixing Shi, Yunbo Lu, Hao Xu, Renfang He, Xiaozhou Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title | Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title_full | Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title_fullStr | Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title_full_unstemmed | Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title_short | Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
title_sort | nuclear transcription factor nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346678/ https://www.ncbi.nlm.nih.gov/pubmed/27788496 http://dx.doi.org/10.18632/oncotarget.12860 |
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