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PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer
Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2′-[(18)F]-fluoro-2′-deoxy-D-glucose ((18)F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb isch...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347009/ https://www.ncbi.nlm.nih.gov/pubmed/28287126 http://dx.doi.org/10.1038/srep44233 |
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author | MacAskill, Mark G. Tavares, Adriana S. Wu, Junxi Lucatelli, Christophe Mountford, Joanne C. Baker, Andrew H. Newby, David E. Hadoke, Patrick W. F. |
author_facet | MacAskill, Mark G. Tavares, Adriana S. Wu, Junxi Lucatelli, Christophe Mountford, Joanne C. Baker, Andrew H. Newby, David E. Hadoke, Patrick W. F. |
author_sort | MacAskill, Mark G. |
collection | PubMed |
description | Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2′-[(18)F]-fluoro-2′-deoxy-D-glucose ((18)F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3′-deoxy-3′-L-[(18)F]-fluorothymidine ((18)F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention. Human endothelial cells (HUVECs) were incubated with (18)F-FDG or (18)F-FLT and cell characteristics were evaluated. Dynamic positron emission tomography (PET) images were acquired post-injection of free (18)F-FDG/(18)F-FLT or (18)F-FDG/(18)F-FLT-labelled HUVECs, following the surgical induction of mouse hind-limb ischemia. In vitro, radiotracer incorporation and efflux was similar with no effect on cell viability, function or proliferation under optimised conditions (5 MBq/mL, 60 min). Injection of free radiotracer demonstrated a faster clearance of (18)F-FLT from the injection site vs. (18)F-FDG (p ≤ 0.001), indicating local cellular uptake. Using (18)F-FLT-labelling, estimation of HUVEC retention within the engraftment site 4 hr post-administration was 24.5 ± 3.2%. PET cell tracking using (18)F-FLT labelling is an improved approach vs. (18)F-FDG as it is not susceptible to local host cell reuptake, resulting in a more accurate estimation of cell retention. |
format | Online Article Text |
id | pubmed-5347009 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53470092017-03-14 PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer MacAskill, Mark G. Tavares, Adriana S. Wu, Junxi Lucatelli, Christophe Mountford, Joanne C. Baker, Andrew H. Newby, David E. Hadoke, Patrick W. F. Sci Rep Article Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2′-[(18)F]-fluoro-2′-deoxy-D-glucose ((18)F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3′-deoxy-3′-L-[(18)F]-fluorothymidine ((18)F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention. Human endothelial cells (HUVECs) were incubated with (18)F-FDG or (18)F-FLT and cell characteristics were evaluated. Dynamic positron emission tomography (PET) images were acquired post-injection of free (18)F-FDG/(18)F-FLT or (18)F-FDG/(18)F-FLT-labelled HUVECs, following the surgical induction of mouse hind-limb ischemia. In vitro, radiotracer incorporation and efflux was similar with no effect on cell viability, function or proliferation under optimised conditions (5 MBq/mL, 60 min). Injection of free radiotracer demonstrated a faster clearance of (18)F-FLT from the injection site vs. (18)F-FDG (p ≤ 0.001), indicating local cellular uptake. Using (18)F-FLT-labelling, estimation of HUVEC retention within the engraftment site 4 hr post-administration was 24.5 ± 3.2%. PET cell tracking using (18)F-FLT labelling is an improved approach vs. (18)F-FDG as it is not susceptible to local host cell reuptake, resulting in a more accurate estimation of cell retention. Nature Publishing Group 2017-03-13 /pmc/articles/PMC5347009/ /pubmed/28287126 http://dx.doi.org/10.1038/srep44233 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article MacAskill, Mark G. Tavares, Adriana S. Wu, Junxi Lucatelli, Christophe Mountford, Joanne C. Baker, Andrew H. Newby, David E. Hadoke, Patrick W. F. PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title | PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title_full | PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title_fullStr | PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title_full_unstemmed | PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title_short | PET Cell Tracking Using (18)F-FLT is Not Limited by Local Reuptake of Free Radiotracer |
title_sort | pet cell tracking using (18)f-flt is not limited by local reuptake of free radiotracer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347009/ https://www.ncbi.nlm.nih.gov/pubmed/28287126 http://dx.doi.org/10.1038/srep44233 |
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