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Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant
The anaerobic protozoan parasite Entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle DNA. Mitosomes play an important role in the life cycle of the parasite. The distribution of proteins in mitosomes is not uniform, and how mitosomes are maintaine...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347163/ https://www.ncbi.nlm.nih.gov/pubmed/28287148 http://dx.doi.org/10.1038/srep44273 |
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author | Kazama, Makoto Ogiwara, Sanae Makiuchi, Takashi Yoshida, Kazuhiro Nakada-Tsukui, Kumiko Nozaki, Tomoyoshi Tachibana, Hiroshi |
author_facet | Kazama, Makoto Ogiwara, Sanae Makiuchi, Takashi Yoshida, Kazuhiro Nakada-Tsukui, Kumiko Nozaki, Tomoyoshi Tachibana, Hiroshi |
author_sort | Kazama, Makoto |
collection | PubMed |
description | The anaerobic protozoan parasite Entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle DNA. Mitosomes play an important role in the life cycle of the parasite. The distribution of proteins in mitosomes is not uniform, and how mitosomes are maintained and retained is unknown. To answer these questions, we developed a transplant method for mitosomes with hemagglutinin-tagged protein into recipient cells containing mitosomes with Myc-tagged protein. Immunofluorescence staining showed that the two protein tags colocalized in single mitosomes in some recipient cells. These results suggest that our transplant method can be used in anaerobic protozoa and that donor mitosomes may obtain recipient proteins through fusion with other mitosomes or through de novo synthesis of proteins in recipient cells. |
format | Online Article Text |
id | pubmed-5347163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53471632017-03-14 Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant Kazama, Makoto Ogiwara, Sanae Makiuchi, Takashi Yoshida, Kazuhiro Nakada-Tsukui, Kumiko Nozaki, Tomoyoshi Tachibana, Hiroshi Sci Rep Article The anaerobic protozoan parasite Entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle DNA. Mitosomes play an important role in the life cycle of the parasite. The distribution of proteins in mitosomes is not uniform, and how mitosomes are maintained and retained is unknown. To answer these questions, we developed a transplant method for mitosomes with hemagglutinin-tagged protein into recipient cells containing mitosomes with Myc-tagged protein. Immunofluorescence staining showed that the two protein tags colocalized in single mitosomes in some recipient cells. These results suggest that our transplant method can be used in anaerobic protozoa and that donor mitosomes may obtain recipient proteins through fusion with other mitosomes or through de novo synthesis of proteins in recipient cells. Nature Publishing Group 2017-03-13 /pmc/articles/PMC5347163/ /pubmed/28287148 http://dx.doi.org/10.1038/srep44273 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kazama, Makoto Ogiwara, Sanae Makiuchi, Takashi Yoshida, Kazuhiro Nakada-Tsukui, Kumiko Nozaki, Tomoyoshi Tachibana, Hiroshi Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title | Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title_full | Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title_fullStr | Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title_full_unstemmed | Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title_short | Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant |
title_sort | behavior of dna-lacking mitochondria in entamoeba histolytica revealed by organelle transplant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347163/ https://www.ncbi.nlm.nih.gov/pubmed/28287148 http://dx.doi.org/10.1038/srep44273 |
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