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Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males

BACKGROUND: Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and sperm apoptosis. Tumor necrosis factor alpha (TNFα) is one of the most-documented cytokines that is involved in spermatogenesis. We investigated the association of the TNFα -308 G/A sin...

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Autores principales: Khademi Bami, Maryam, Dehghan Tezerjani, Masoud, Montazeri, Fateme, Ashrafzadeh Mehrjardi, Hamid Reza, Ghasemi-Esmailabad, Saeed, Sheikhha, Mohammad Hasan, Kalantar, Seyed Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347448/
https://www.ncbi.nlm.nih.gov/pubmed/28670429
http://dx.doi.org/10.22074/ijfs.2017.4830
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author Khademi Bami, Maryam
Dehghan Tezerjani, Masoud
Montazeri, Fateme
Ashrafzadeh Mehrjardi, Hamid Reza
Ghasemi-Esmailabad, Saeed
Sheikhha, Mohammad Hasan
Kalantar, Seyed Mehdi
author_facet Khademi Bami, Maryam
Dehghan Tezerjani, Masoud
Montazeri, Fateme
Ashrafzadeh Mehrjardi, Hamid Reza
Ghasemi-Esmailabad, Saeed
Sheikhha, Mohammad Hasan
Kalantar, Seyed Mehdi
author_sort Khademi Bami, Maryam
collection PubMed
description BACKGROUND: Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and sperm apoptosis. Tumor necrosis factor alpha (TNFα) is one of the most-documented cytokines that is involved in spermatogenesis. We investigated the association of the TNFα -308 G/A single nucleotide polymorphism with sperm abnormalities in Iranian males. MATERIALS AND METHODS: This case-control study included 180 infertile men who re- ferred to Yazd Research and Clinical Center for Infertility and 100 healthy normospermic controls. Infertile men were classified into four groups of azoospermia (n=91), oligospermia (n=26), teratospermia (n=30) and asthenoteratospermia (n=33). After sperm analysis, DNA was extracted from blood and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for the genotyping of TNFα- 308 G/A. RESULTS: The A allele was significantly associated with sperm abnormality in our population [(P<0.001, odds ratios (OR) 95% confidence interval (CI)=2.31]. In addition, the A allele was also associated with azoospermia (P<0.001, OR (95% CI)=2.484), oligospermia (P=0.005, OR (95% CI)=2.51) and teratospemia (P<0.001, OR (95% CI)=3.385) but not with asthenoteratospermia (P=0.623). CONCLUSION: Our data suggest that this single nucleotide polymorphism (SNP) maybe associated with the risk of sperm abnormality in infertile men of Iranian origin.
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spelling pubmed-53474482017-07-01 Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males Khademi Bami, Maryam Dehghan Tezerjani, Masoud Montazeri, Fateme Ashrafzadeh Mehrjardi, Hamid Reza Ghasemi-Esmailabad, Saeed Sheikhha, Mohammad Hasan Kalantar, Seyed Mehdi Int J Fertil Steril Original Article BACKGROUND: Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and sperm apoptosis. Tumor necrosis factor alpha (TNFα) is one of the most-documented cytokines that is involved in spermatogenesis. We investigated the association of the TNFα -308 G/A single nucleotide polymorphism with sperm abnormalities in Iranian males. MATERIALS AND METHODS: This case-control study included 180 infertile men who re- ferred to Yazd Research and Clinical Center for Infertility and 100 healthy normospermic controls. Infertile men were classified into four groups of azoospermia (n=91), oligospermia (n=26), teratospermia (n=30) and asthenoteratospermia (n=33). After sperm analysis, DNA was extracted from blood and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for the genotyping of TNFα- 308 G/A. RESULTS: The A allele was significantly associated with sperm abnormality in our population [(P<0.001, odds ratios (OR) 95% confidence interval (CI)=2.31]. In addition, the A allele was also associated with azoospermia (P<0.001, OR (95% CI)=2.484), oligospermia (P=0.005, OR (95% CI)=2.51) and teratospemia (P<0.001, OR (95% CI)=3.385) but not with asthenoteratospermia (P=0.623). CONCLUSION: Our data suggest that this single nucleotide polymorphism (SNP) maybe associated with the risk of sperm abnormality in infertile men of Iranian origin. Royan Institute 2017 2017-02-16 /pmc/articles/PMC5347448/ /pubmed/28670429 http://dx.doi.org/10.22074/ijfs.2017.4830 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Khademi Bami, Maryam
Dehghan Tezerjani, Masoud
Montazeri, Fateme
Ashrafzadeh Mehrjardi, Hamid Reza
Ghasemi-Esmailabad, Saeed
Sheikhha, Mohammad Hasan
Kalantar, Seyed Mehdi
Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title_full Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title_fullStr Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title_full_unstemmed Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title_short Tumor Necrosis Factor Alpha -308 G/A Single Nucleotide Polymorphism and Risk of Sperm Abnormalities in Iranian Males
title_sort tumor necrosis factor alpha -308 g/a single nucleotide polymorphism and risk of sperm abnormalities in iranian males
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347448/
https://www.ncbi.nlm.nih.gov/pubmed/28670429
http://dx.doi.org/10.22074/ijfs.2017.4830
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