Cargando…

Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse

The decline of quantity and quality of sperm are correlated with the increasing age and some anti-cancer compounds such as busulfan. Previous studies have shown that Resveratrol (Res) inhibits tumorigenesis and metastasis of many cancers including mammary tumor, prostate and pancreatic cancers. It a...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Chongyang, Zhang, Ying, Shen, Qiaoyan, Zhou, Zhe, Liu, Weishuai, Hua, Jinlian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347676/
https://www.ncbi.nlm.nih.gov/pubmed/27806317
http://dx.doi.org/10.18632/oncotarget.12990
_version_ 1782514084654088192
author Wu, Chongyang
Zhang, Ying
Shen, Qiaoyan
Zhou, Zhe
Liu, Weishuai
Hua, Jinlian
author_facet Wu, Chongyang
Zhang, Ying
Shen, Qiaoyan
Zhou, Zhe
Liu, Weishuai
Hua, Jinlian
author_sort Wu, Chongyang
collection PubMed
description The decline of quantity and quality of sperm are correlated with the increasing age and some anti-cancer compounds such as busulfan. Previous studies have shown that Resveratrol (Res) inhibits tumorigenesis and metastasis of many cancers including mammary tumor, prostate and pancreatic cancers. It acts as anti-age in mouse and human, however, little is known about its protective effect on aged spermatogonial stem cells (SSCs). Here, we investigated the effects of Res in vitro on SSCs using C18-4 cells and in vivo in busulfan-induced azoospermia mice model. The results showed that Res at different concentrations had different effects on C18-4 cells. Treatment with 2 μM of Res promotes cell proliferation and inhibits apoptosis, but stimulates apoptosis with a higher concentration (20 μM) in C18-4 cells. Using busulfan-induced infertility mice model, we demonstrated that Res (30 mg/kg/d and 100 mg/kg/d) clearly ameliorated SSC loss to recover the spermatogenesis. Taken together, our data suggest that Res might be an approach for therapeutic intervention to promote SSC proliferation and cease SSC loss in azoospermia mice model induced by busulfan.
format Online
Article
Text
id pubmed-5347676
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53476762017-03-31 Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse Wu, Chongyang Zhang, Ying Shen, Qiaoyan Zhou, Zhe Liu, Weishuai Hua, Jinlian Oncotarget Research Paper: Pathology The decline of quantity and quality of sperm are correlated with the increasing age and some anti-cancer compounds such as busulfan. Previous studies have shown that Resveratrol (Res) inhibits tumorigenesis and metastasis of many cancers including mammary tumor, prostate and pancreatic cancers. It acts as anti-age in mouse and human, however, little is known about its protective effect on aged spermatogonial stem cells (SSCs). Here, we investigated the effects of Res in vitro on SSCs using C18-4 cells and in vivo in busulfan-induced azoospermia mice model. The results showed that Res at different concentrations had different effects on C18-4 cells. Treatment with 2 μM of Res promotes cell proliferation and inhibits apoptosis, but stimulates apoptosis with a higher concentration (20 μM) in C18-4 cells. Using busulfan-induced infertility mice model, we demonstrated that Res (30 mg/kg/d and 100 mg/kg/d) clearly ameliorated SSC loss to recover the spermatogenesis. Taken together, our data suggest that Res might be an approach for therapeutic intervention to promote SSC proliferation and cease SSC loss in azoospermia mice model induced by busulfan. Impact Journals LLC 2016-10-29 /pmc/articles/PMC5347676/ /pubmed/27806317 http://dx.doi.org/10.18632/oncotarget.12990 Text en Copyright: © 2016 Wu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Wu, Chongyang
Zhang, Ying
Shen, Qiaoyan
Zhou, Zhe
Liu, Weishuai
Hua, Jinlian
Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title_full Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title_fullStr Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title_full_unstemmed Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title_short Resveratrol changes spermatogonial stem cells (SSCs) activity and ameliorates their loss in busulfan-induced infertile mouse
title_sort resveratrol changes spermatogonial stem cells (sscs) activity and ameliorates their loss in busulfan-induced infertile mouse
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347676/
https://www.ncbi.nlm.nih.gov/pubmed/27806317
http://dx.doi.org/10.18632/oncotarget.12990
work_keys_str_mv AT wuchongyang resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse
AT zhangying resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse
AT shenqiaoyan resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse
AT zhouzhe resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse
AT liuweishuai resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse
AT huajinlian resveratrolchangesspermatogonialstemcellssscsactivityandamelioratestheirlossinbusulfaninducedinfertilemouse